Evaluation of the Antioxidant Activity of Cell Extracts  from Microalgae

Guedes, A. Catarina; Gião, Maria S.; Seabra, Rui; Ferreira, Ana Letícia Andrade; Tamagnini, Paula; Moradas‐Ferreira, Pedro; Malcata, F. Xavier

doi:10.3390/md11041256

Cited by 70 publications

(42 citation statements)

References 30 publications

(32 reference statements)

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“…Higher amount of phenolics were found in lipid extracts of Acutodesmus and significant antioxidant levels was observed thus confirming the role of phenolic compounds in antioxidant properties. Similar results from Scenedesmus were obtained by previous studies (Guedes et al, 2013;Aboul-Enein et al, 2003) with higher antioxidants levels and activity. DPPH radical is widely used to test the free radical-scavenging ability of various samples.…”

Section: Discussionsupporting

confidence: 91%

Variations among Antioxidant Profiles in Lipid and Phenolic Extracts of Microalgae from Different Growth Medium

Shetty¹,

Mokashi²,

Sibi³

2015

J. of Fisheries and Aquatic Science

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Up-scaling the production of value added products from microalgae requires reliable techniques. This study has been carried out in order to determine whether microalgae have an inherent tendency to contain similar proportions of antioxidant properties in its lipid and phenolic extracts under varying growth medium. Chlorella vulgaris and Acutodesmus obliquus (Scenedesmus obliquus) were cultivated in normal water, Bold's medium and sewage water followed by extraction of lipids and phenolics. The extracts were subjected to in vitro antioxidant assays performed in triplicates in which higher scavenging activity of DPPH and super oxide radical was observed in phenolic extracts. However, there was a significantly higher antioxidant potential found in lipid extracts suggests that next to the well studied phenolic compounds, microalgal lipids should also be considered when using microalgae as a source of natural antioxidants. Further, antioxidant profile of lipid and phenolic extracts from same species varied with growth medium.

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Section: Discussionsupporting

confidence: 91%

Variations among Antioxidant Profiles in Lipid and Phenolic Extracts of Microalgae from Different Growth Medium

Shetty¹,

Mokashi²,

Sibi³

2015

J. of Fisheries and Aquatic Science

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“…Cyanobacteria could be one of the most potential source for the production of natural antioxidants. Phenolic acids and flavonoids from plant sources have been well-documented for their antioxidant, free-radical quenching and redox metal ion-chelating affects (Singh et al 2009(Singh et al , 2014Tutour 1990;Apati et al 2003;Hossain et al 2016) but cyanobacteria and microalgae are relatively new source for such compounds in recent years due to their potential benefits and ease of production (Guedes et al 2013;Machu et al 2015). Gallic, chlorogenic, caffeic and ferulic acids identified from plant sources have been known as strong antioxidants and free radical scavengers (Piazzon et al 2012;Babić et al 2015) but their presence in cyanobacteria is scarcely reported and this make these organisms more potential.…”

Section: Resultsmentioning

confidence: 99%

“…Many species exhibit wide range & Dhananjaya P. Singh nabg.nbaim@gmail.com of chemical diversity with characteristic structures coupled with bioactivity that make these organisms a potential candidate for pharmaceuticals (Mandal and Rath 2015). Cyanobacterial species also exhibit potential adaptation strategies against oxidative stresses mainly by the activation of antioxidant defense system that comprises activation of various enzymes (superoxide dismutase, catalase, glutathione reductase and ascorbate peroxidase) (Bharanidharan et al 2013;Guedes et al 2013). Phenylpropanoids and their derivatives are probably the largest group of secondary metabolites isolated from natural sources (plants, microorganisms and microalgal cultures) (Wojdylo et al 2007;Vogt 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Cyanobacteria are promising prokaryotic photosynthetic microorganisms that act as potential cell factories producing a wide array of secondary metabolites of different class and groups including venturamideA, dragomabin, dragonamide -A, -B, carmabin -A, -B, calothrixins -A, -B, dolastatins and nostocarboline (Rastogi and Sinha 2009a, b;Guedes et al 2013;Xue and He 2015). Additionally, these organisms have the capabilities to express plant P450 proteins that have essential functions in the biosynthesis of natural product molecules (Xue and He 2015).…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant properties and polyphenolic content in terrestrial cyanobacteria

et al. 2017

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Cell-free extracts of twenty terrestrial cyanobacteria were evaluated for their antioxidant properties in terms of free-radical scavenging (DPPH and ABTS) and metal chelating activity and deoxyribose protection. Extract of Anabaena constricta was the most prominent antioxidant agent (IC 50 for DPPH activity 0.91 mg ml -1 , ABTS 0.23 mg ml -1 , deoxyribose protection 0.63 mg ml -1 and Fe ?2 -ion chelating 0.9 mg ml -1 ). The extracts of cyanobacterial species contained high quantity of total phenol and total flavonoid that were supposed to impart prominent antioxidant properties. Cyanobacterial species also showed fairly high PAL activity. We reported varied quantities of polyphenolics gallic, chlorogenic, caffeic, vanillic and ferulic acids and flavonoids rutin, quercetin and kaempferol in cyanobacterial extracts. The presence of these polyphenolics was linked with the free radical scavenging, metal chelating and antioxidative damage protecting properties of the organisms. Cyanobacteria are the most feasible, promising and alternative candidates for searching out new chemical leads for industrial applications in pharmaceuticals, neutraceuticals and biomolecules of importance. Presence of biomolecules such as polyphenolics and their connection with the prominent biological functions (e.g., antioxidant properties) make these organisms a potential source of secondary metabolites with predominant biological activities. Additionally, dominant presence of polyphenols as antioxidant agents in cyanobacterial species may reflect their adaptation strategies against abiotic stresses for their ecological success in different habitats.

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“…Several studies has been indicated that protein content in most of green microalgae is higher than plants (Becker, 1994;Becker, 2007). Microalgae consequently gift to humans and animals positive healthy and reproductive effects (Becker, 2007;Guedes et al, 2013;Choochote et al, 2014;Senosy et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Development of Oocytes and Preimplantation Embryos of Mice Fed Diet Supplemented with Dunaliella salina

Mohammed¹

2017

Adv. Anim. Vet. Sci.

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| The present study investigated the developmental competence of germinal vesicle (GV) oocytes and the resulting embryos upon dietary Dunaliella salina (DS) supplementation. Quality of GV oocytes and maturation, timing of zygotes cleavage to two-cell stage, quality of embryos and offspring number and weight were investigated of mice supplemented with DS (100g/kg ration; N=30) compared to control (N=30).Females were injected with 7.5 I.U. of equine chorionic gonadotropin (PMSG) followed by 7.5 I.U. of human chorionic gonadotropin (hCG) after 48h and mated with fertile male. Immature GV oocytes were harvested from ovaries after 48h of PMSG injection for investigating quality of oocyte, timing of breakdown of germinal vesicle (GVBD) and extrusion of first polar bodies and maturation rate (%).Zygotes were harvested from uterine tubes 29-30hafterhCG injection and followed for first cleavage whereas quality of blastocysts were evaluated after collection from uterine horn at96h of hCG. Improvement of oocyte quality has been indicated in DS group whereas GVBD and maturation rate were not differed between DS and control groups. Although D. salina did not change timing of first cleavage of zygotes, blastocysts' quality were significantly (P<0.05) increased in DS group. Furthermore, offspring number (9.9 ± 0.26 & 8.2 ± 0.30) and weight (12.01 ± 0.31 & 9.84 ± 0.37) at birth were increased significantly (P<0.05) in the DS group compared to control one. In conclusion, supplementation of D. salina could increase reproductive performance through improvement quality of germinal vesicle oocytes and preimplantation embryos.

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Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae

Cited by 70 publications

References 30 publications

Variations among Antioxidant Profiles in Lipid and Phenolic Extracts of Microalgae from Different Growth Medium

Variations among Antioxidant Profiles in Lipid and Phenolic Extracts of Microalgae from Different Growth Medium

Antioxidant properties and polyphenolic content in terrestrial cyanobacteria

Development of Oocytes and Preimplantation Embryos of Mice Fed Diet Supplemented with Dunaliella salina

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