Evaluation of the antimicrobial effect of lactoferrin on Porphyromonas gingivalis, Prevotella intermedia and Prevotella nigrescens

Aguilera, O

doi:10.1016/s0928-8244(98)00030-3

Cited by 16 publications

(22 citation statements)

References 18 publications

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“…In these investigations, it was reported that lactoferrin possessed antibacterial activities against many kinds of bacteria, such as Helicobactor pylori, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, and Staphylococcus epidermidis, as well as fungi, including C. albicans (2,60,62). In 1992, Bellamy et al (5) synthesized a potent antifungal lactoferrin peptide consisting of 25 amino acid residues, lactoferricin B, which lacked ironbinding activity.…”

Section: Discussionmentioning

confidence: 99%

“…The mechanism of the antifungal activity and the pharmodynamics of peptide 2 have not yet been sufficiently explored. To establish a reasonable combined treatment of candidal infections with peptide 2 and other agents, the antifungal mechanism of peptide 2, including its influence on CDR1, CDR2, HWP1, and the enhanced filamentous growth 1 gene, which regulate candidal virulence, remains to be studied.…”

Section: Discussionmentioning

confidence: 99%

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Lactoferrin Peptide Increases the Survival of Candida albicans - Inoculated Mice by Upregulating Neutrophil and Macrophage Functions, Especially in Combination with Amphotericin B and Granulocyte-Macrophage Colony-Stimulating Factor

et al. 2001

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To develop a new strategy to control candidiasis, we examined in vivo the anticandidal effects of a synthetic lactoferrin peptide, FKCRRWQWRM (peptide 2) and the peptide that mimics it, FKARRWQWRM (peptide 2). Although all mice that underwent intraperitoneal injection of 5 ؋ 10 8 Candida cells with or without peptide 2 died within 8 or 7 days, respectively, the survival times of mice treated with 5 to 100 g of intravenous peptide 2 per day for 5 days after the candidal inoculation were prolonged between 8.4 ؎ 2.9 and 22.4 ؎ 3.6 days, depending on the dose of peptide 2. The prolongation of survival by peptide 2 was also observed in mice that were infected with 1.0 ؋ 10 9 Candida albicans cells (3.2 ؎ 1.3 days in control mice versus 8.2 ؎ 2.4 days in the mice injected with 10 g of peptide 2 per day). In the high-dose inoculation, a combination of peptide 2 (10 g/day) with amphotericin B (0.1 g/day) and granulocyte-macrophage colony-stimulating factor (GM-CSF) (0.1 g/day) brought prolonged survival. With a combination of these agents, 60% of the mice were alive for more than 22 days. Correspondingly, peptide 2 activated phagocytes inducing inducible NO synthase and the expression of p47 phox and p67 phox , and peptide 2 increased phagocyte Candida-killing activities up to 1.5-fold of the control levels upregulating the generation of superoxide, lactoferrin, and defensin from neutrophils and macrophages. These findings indicated that the anticandidal effects of peptide 2 depend not only on the direct Candida cell growth-inhibitory activity, but also on the phagocytes' upregulatory activity, and that combinations of peptide 2 with GM-CSF and antifungal drugs will help in the development of new strategies for control of candidiasis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Lactoferrin Peptide Increases the Survival of Candida albicans - Inoculated Mice by Upregulating Neutrophil and Macrophage Functions, Especially in Combination with Amphotericin B and Granulocyte-Macrophage Colony-Stimulating Factor

et al. 2001

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“…Therefore, LF has been studied for its activity against oral pathogens, including Streptococcus mutans (7), Candida albicans (38,41), and periodontopathic bacteria (2,21). Porphyromonas gingivalis and Prevetella intermedia are gram-negative black-pigmenting anaerobes that are etiologic agents of chronic adult periodontitis (26) and that reside as biofilms in the subgingival plaque (14,27).…”

mentioning

confidence: 99%

“…Porphyromonas gingivalis and Prevetella intermedia are gram-negative black-pigmenting anaerobes that are etiologic agents of chronic adult periodontitis (26) and that reside as biofilms in the subgingival plaque (14,27). hLF was shown to exhibit growth-inhibitory activity against P. gingivalis but not P. intermedia (2,35), and LFcin B was found to have weak activity against P. gingivalis (35). hLF and bLF also inhibited the adhesion of P. intermedia to epithelial cells (4).…”

mentioning

confidence: 99%

Inhibitory Effects of Lactoferrin on Growth and Biofilm Formation ofPorphyromonas gingivalisandPrevotella intermedia

Wakabayashi

Yamauchi

Kobayashi

et al. 2009

Antimicrob Agents Chemother

114

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Lactoferrin (LF) is an iron-binding antimicrobial protein present in saliva and gingival crevicular fluids, and it is possibly associated with host defense against oral pathogens, including periodontopathic bacteria. In the present study, we evaluated the in vitro effects of LF-related agents on the growth and biofilm formation of two periodontopathic bacteria, Porphyromonas gingivalis and Prevotella intermedia, which reside as biofilms in the subgingival plaque. The planktonic growth of P. gingivalis and P. intermedia was suppressed for up to 5 h by incubation with >130 g/ml of human LF (hLF), iron-free and iron-saturated bovine LF (apo-bLF and holo-bLF, respectively), and >6 g/ml of bLF-derived antimicrobial peptide lactoferricin B (LFcin B); but those effects were weak after 8 h. The biofilm formation of P. gingivalis and P. intermedia over 24 h was effectively inhibited by lower concentrations (>8 g/ml) of various iron-bound forms (the apo, native, and holo forms) of bLF and hLF but not LFcin B. A preformed biofilm of P. gingivalis and P. intermedia was also reduced by incubation with various iron-bound bLFs, hLF, and LFcin B for 5 h. In an examination of the effectiveness of native bLF when it was used in combination with four antibiotics, it was found that treatment with ciprofloxacin, clarithromycin, and minocycline in combination with native bLF for 24 h reduced the amount of a preformed biofilm of P. gingivalis compared with the level of reduction achieved with each agent alone. These results demonstrate the antibiofilm activity of LF with lower iron dependency against P. gingivalis and P. intermedia and the potential usefulness of LF for the prevention and treatment of periodontal diseases and as adjunct therapy for periodontal diseases.

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“…Lactoferrin (Lf) was first found more than 30 years ago in human maternal milk, and its antimicrobial activity was ascertained soon thereafter (1,34,41,42,45). About 10 years later, the peptides lactoferricins B and H were synthesized, along with the bovine and human Lf amino acid sequences.…”

mentioning

confidence: 99%

Regulation of Fungal Infection by a Combination of Amphotericin B and Peptide 2, a Lactoferrin Peptide That Activates Neutrophils

Okamoto

Tanida

Ben-juan

et al. 2004

Clin Vaccine Immunol

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To establish a novel strategy for the control of fungal infection, we examined the antifungal and neutrophilactivating activities of antimicrobial peptides. The duration of survival of 50% of mice injected with a lethal dose of Candida albicans (5 ؋ 10 8 cells) or Aspergillus fumigatus (1 ؋ 10 8 cells) was prolonged 3 to 5 days by the injection of 10 g of peptide 2 (a lactoferrin peptide) and 10 g of ␣-defensin 1 for five consecutive days and was prolonged 5 to 13 days by the injection of 0.1 g of granulocyte-monocyte colony-stimulating factor (GM-CSF) and 0.5 g of amphotericin B. When mice received a combined injection of peptide 2 (10 g/day) with amphotericin B (0.5 g/day) for 5 days after the lethal fungal inoculation, their survival was greatly prolonged and some mice continued to live for more than 5 weeks, although the effective doses of peptide 2 for 50 and 100% suppression of Candida or Aspergillus colony formation were about one-third and one-half those of amphotericin B, respectively. In vitro, peptide 2 as well as GM-CSF increased the Candida and Aspergillus killing activities of neutrophils, but peptides such as ␣-defensin 1, ␤-defensin 2, and histatin 5 did not upregulate the killing activity. GM-CSF together with peptide 2 but not other peptides enhanced the production of superoxide (O 2 ؊ ) by neutrophils. The upregulation by peptide 2 was confirmed by the activation of the O 2 ؊ -generating pathway, i.e., activation of large-molecule guanine binding protein, phosphatidyl-inositol 3-kinase, protein kinase C, and p47 phox as well as p67 phox . In conclusion, different from natural antimicrobial peptides, peptide 2 has a potent neutrophil-activating effect which could be advantageous for its clinical use in combination with antifungal drugs.

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Evaluation of the antimicrobial effect of lactoferrin on Porphyromonas gingivalis, Prevotella intermedia and Prevotella nigrescens

Cited by 16 publications

References 18 publications

Lactoferrin Peptide Increases the Survival of Candida albicans - Inoculated Mice by Upregulating Neutrophil and Macrophage Functions, Especially in Combination with Amphotericin B and Granulocyte-Macrophage Colony-Stimulating Factor

Lactoferrin Peptide Increases the Survival of Candida albicans - Inoculated Mice by Upregulating Neutrophil and Macrophage Functions, Especially in Combination with Amphotericin B and Granulocyte-Macrophage Colony-Stimulating Factor

Inhibitory Effects of Lactoferrin on Growth and Biofilm Formation ofPorphyromonas gingivalisandPrevotella intermedia

Regulation of Fungal Infection by a Combination of Amphotericin B and Peptide 2, a Lactoferrin Peptide That Activates Neutrophils

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