Evaluation of the Antimicrobial Activity of Nanostructured Materials of Titanium Dioxide Doped with Silver and/or Copper and Their Effects on<i>Arabidopsis thaliana</i>

Garcidueñas-Piña, Cristina; Medina-Ramírez, Iliana E.; Guzmán, Plinio; Rico‐Martínez, Roberto; Morales-Domínguez, José Francisco; Rubio-Franchini, Isidoro

doi:10.1155/2016/8060847

Cited by 29 publications

(7 citation statements)

References 43 publications

(51 reference statements)

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“…However, we aim to develop robust NMs that exert antifungal activity through diverse mechanisms; TiO 2 -Cu 2+ /CuI. Our results demonstrate the enhanced antifungal activity of the TiO 2 -Cu 2+ /CuI composite; we can observe that the incorporation of TiO 2 enhanced the CuI activity, thus obtaining a lower MFC of 10 µg/mL, attributed to the TiO 2 -Cu 2+ antimicrobial activity and capacity to form reactive oxygen species (ROS) [ 11 , 41 ].…”

Section: Resultsmentioning

confidence: 97%

Synthesis of TiO2-Cu2+/CuI Nanocomposites and Evaluation of Antifungal and Cytotoxic Activity

et al. 2023

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Fungal infections have become a significant public health concern due to their increasing recurrence and harmful effects on plants, animals, and humans. Opportunistic pathogens (among others from the genera Candida and Aspergillus) can be present in indoor air, becoming a risk for people with suppressed immune systems. Engineered nanomaterials are novel alternatives to traditional antifungal therapy. In this work, copper(I) iodide (CuI) and a copper-doped titanium dioxide—copper(I) iodide (TiO2-Cu2+/CuI) composite nanomaterials (NMs)—were synthesized and tested as antifungal agents. The materials were synthesized using sol-gel (TiO2-Cu2+) and co-precipitation (CuI) techniques. The resulting colloids were evaluated as antifungal agents against Candida parapsilosis and Aspergillus niger strains. The NMs were characterized by XRD, HRTEM, AFM, and DLS to evaluate their physicochemical properties. The NMs present a high size dispersion and different geometrical shapes of agglomerates. The antifungal capacity of the NMs by the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) was below 15 µg/mL against Candida parapsilosis and below 600 µg/mL against Aspergillus niger for both NMs. Holotomography microscopy showed that the NMs could penetrate cell membranes causing cell death through its rupture and reactive oxygen species (ROS) production. Cytotoxicity tests showed that NMs could be safe to use at low concentrations. The synthesized nanomaterials could be potential antifungal agents for biomedical or environmental applications.

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Section: Resultsmentioning

confidence: 97%

Synthesis of TiO2-Cu2+/CuI Nanocomposites and Evaluation of Antifungal and Cytotoxic Activity

et al. 2023

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“…The aerobic mesophilic microorganisms were counted using the plate pouring technique on the agar medium for standard methods. One ml of the dilutions of each water treatment in 20 ml of agar medium for standard methods was added and incubated at 37°C for 24-48 h. After that time, the count of the colonies was carried out and the log inhibition (log10 N0/N) was calculated for each treatment; where N0 is the initial count, and N is the count after treatment [36]. The results were analyzed by means of a one-way ANOVA followed by a Tukey test (α < 0:05).…”

Section: Microbiological Analysismentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles from the Opuntia ficus-indica Fruit and Its Activity against Treated Wastewater Microorganisms

Muñoz-Carrillo

Garcidueñas-Piña

Valerio-García

et al. 2020

Journal of Nanomaterials

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Wastewater can be reused after a treatment process and compliance with high quality standards that guarantee its safe use. The wastewater treatment plant of the Autonomous University of Aguascalientes (AUA), like others, uses primary, secondary, and tertiary processes. The tertiary process followed is chlorination and is used to eliminate microorganisms from the secondary process. Although water of acceptable quality is obtained with chlorine, there is evidence that toxic substances are generated when reacting with organic matter, so alternatives to the use of chlorination have been analyzed. In the present study, silver nanoparticles were synthesized from the aqueous extract of the Opuntia ficus indica fruit peel (OfAgNPs), by reducing a 2 mM solution of AgNO3. OfAgNPs were characterized by UV-visible spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, atomic absorption spectroscopy, and dynamic light scattering, in addition to his electrophoretic mobility. The OfAgNPs are spherical, with an average particle size distribution of 64.28 ± 11.82 nm, relatively stable at room temperature, negatively charged ( − 25.1 ± 0.03 mV), and composed of 61.29% silver. The activity of OfAgNPs was evaluated in water from the effluent of the AUA treatment plant, before and after chlorination, and inhibition of bacteria Escherichia coli var 1, Enterobacter aerogenes var 1, Citrobacter freudi var 2, atypical E. coli, and aerobic mesophilic microorganism was tested.

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“…After each exposure period, aliquots were taken from the suspension and inoculated in solid LB medium for 19 h at 37 °C and the CFU/mL number was counted. The log inactivation was calculated as log 10 (N/ N 0 ), where N 0 represents the initial CFU/mL and N the CFU/ mL treatment survivors (Garcidueñas-Piña et al, 2016). The experiments were performed for triplicate and maintained under sterile conditions.…”

Section: Figmentioning

confidence: 99%

Heterologous expression of bacteriocin E-760 in Chlamydomonas reinhardtii and functional analysis

Quezada-Rivera

Soria-Guerra

Pérez-Juárez

et al. 2019

Phyton

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The use of antimicrobial peptides (AMPs) synthesized by bacteria (bacteriocins) is an alternative for combating multidrug resistant bacterial strains and their production by recombinant route is a viable option for their mass production. The bacteriocin E-760 isolated from the genus Enterococcus sp. has been shown to possess inhibitory activity against Gram-negative and Gram-positive bacteria. In this study, the expression of a chimeric protein coding for E-760 in the nucleus of C. reinhardtii was evaluated, as well as, its antibacterial activity. The synthetic gene E-760S was inserted into the genome of C. reinhardtii using Agrobacterium tumefaciens. A transgenic line was identified in TAP medium with hygromycin and also by PCR. The increment in the culture medium temperature of the transgenic strain at 35 °C for 10 minutes, increased the production level of the recombinant protein from 0.14 (Noninduced culture, NIC) to 0.36% (Induced culture, IC) of total soluble proteins (TSP); this was quantified by an ELISA assay. Recombinant E-760 possesses activity against Staphylococcus aureus in 0.34 U log, Streptococcus agalactiae in 0.48 U log, Enterococcus faecium in 0.36 U log, Pseudomonas aeruginosa in 2 U log and for Klebsiella pneumoniae, the activity was 0.07 U log. These results demonstrate that the nucleus transformation of C. reinhardtii can function as a stable expression platform for the production of the synthetic gene E-760 and it can potentially be used as an antibacterial agent.

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Evaluation of the Antimicrobial Activity of Nanostructured Materials of Titanium Dioxide Doped with Silver and/or Copper and Their Effects onArabidopsis thaliana

Cited by 29 publications

References 43 publications

Synthesis of TiO2-Cu2+/CuI Nanocomposites and Evaluation of Antifungal and Cytotoxic Activity

Synthesis of TiO2-Cu2+/CuI Nanocomposites and Evaluation of Antifungal and Cytotoxic Activity

Green Synthesis of Silver Nanoparticles from the Opuntia ficus-indica Fruit and Its Activity against Treated Wastewater Microorganisms

Heterologous expression of bacteriocin E-760 in Chlamydomonas reinhardtii and functional analysis

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