Evaluation of the antidiabetic activity of hydrolyzed peptides derived fromJuglans mandshuricaMaxim. fruits in insulin-resistant HepG2 cells and type 2 diabetic mice

Abstract

Bioactive peptides derived from plant proteins are promising sources for the development safe hypoglycemics. The present study was designed to investigate the antidiabetic activity of walnut hydrolyzed peptides (WHPs) obtained from the fruit proteins of Juglans mandshurica Maxim. In vitro results showed that WHPs with medium molecular weights (3-10 kDa) exhibited the highest a-glucosidase inhibitory rate (61.73%) and significantly increased extracellular glucose consumption in insulin-resistant HepG2 cells. In mice with streptozotocin-induced type 2 diabetes, WHPs with medium molecular weights reduced fasting blood glucose levels by 64.82% and increased insulin secretion by 23.71% and liver glucokinase and glycogen levels by 69.54 and 76.19%, respectively. Furthermore, an analysis of serum lipid profiles showed that WHPs significantly decreased serum total cholesterol, triglyceride, and low-density lipoprotein cholesterol levels. Our results indicate the antidiabetic potential of WHPs and provide evidence to support the application of WHPs in the treatment of diabetes. Practical applicationsJuglans mandshurica Maxim., commonly known as the Manchurian walnut, is a member of the Juglandaceae plant family. Its fruit is extensively consumed in northeast China and is used in the production of dairy and bakery products, candy, and cooking oil owing to its high protein content (about 30%), abundance of essential amino acids, and easy digestibility. Hence, there has been an immense increase, up to a million hectare, in the area under Manchurian walnut cultivation. The results of the present study indicated that WHPs showed significant antidiabetic activity by improving a-glucosidase activity, glucose metabolism, insulin secretion, and liver glucokinase and glycogen levels as well as by decreasing the fasting blood glucose level. These findings have identified a green source of novel antidiabetic peptides that can either be used as a functional food ingredient in the management of hyperglycemia or developed as a drug for the treatment of type 2 diabetes.

Manufacture of low-fat Cheddar cheese by exopolysaccharide-producing Lactobacillus plantarum JLK0142 and its functional properties

“…The α-glucosidase inhibition assays were carried out according to the method described by Wang et al (2018b). Briefly, 5 µL of α-glucosidase solution (1.0 U/ mL), 20 µL of WSE, and 165 µL of sodium phosphate buffer (0.1 mM, pH 6.8) were mixed and incubated in a 96-well plate at 37°C for 10 min.…”

Short Tetra‐peptide from soy‐protein hydrolysate attenuates hyperglycemia associated damages in H9c2 cells and ICR mice

“…The severity of diabetes is normally proportional to the levels of cardiac cell death (Ghosh et al, ) and attenuation of apoptosis has shown amelioration of diabetic cardiomyopathy (Tian et al, ; Zhang et al, ). Various studies show the cardio‐protective effects of functional ingredients including protein hydrolysates from different food around the globe to possess cytoprotective effects against DM (Asokan et al, ; Chiang et al, ; Ramos‐Gomez et al, ; Wang et al, ). Irrespective of the effects of VHVV observed on STZ‐induced diabetic mice the tetra peptide showed effective attenuation of apoptosis in H9c2 induced by high glucose supplementation.…”

“…The severity of diabetes is normally proportional to the levels of cardiac cell death (Ghosh et al, 2005) and attenuation of apoptosis has shown amelioration of diabetic cardiomyopathy (Tian et al, 2017;Zhang et al, 2017). Various studies show the cardio-protective effects of functional ingredients including protein hydrolysates from different food around the globe to possess cytoprotective effects against DM Chiang et al, 2014;Ramos-Gomez et al, 2017;Wang et al, 2018).…”

Potential Health Benefits of Plant Food-Derived Bioactive Components: An Overview

“…An in vitro study of WHPs indicated that 3-10 kDa peptides reduced α-glucosidase activity and improved extracellular glucose consumption in HepG2 insulin-resistant cells. During in vivo tests, the WHPs decreased fasting blood glucose levels (64.82%) by improving liver glucokinase, glycogen and insulin secretion by 69.54%, 76.19%, and 23.71%, respectively [71]. A recent study by Barik et al confirmed the potential of anthocyanin-rich black currants (BC) to regulate hyperglycemia using CaCo-2 cells.…”