EVALUATION OF THE ANTIBIOFILM EFFECT OF FLUCONAZOLE LOADED PLGA NANOPARTICLES PREPARED USING RHAMNOLIPID ON Candida albicans

Abstract

In this study, fluconazole(FLZ) loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles(NPs) were prepared with two different formulations consisting of polyvinyl alcohol(PVA) and PVA-rhamnolipid(R) in order to improve antibiofilm activity against Candida albicans ATCC 90028. The encapsulation efficiency, drug loading capacity, in-vitro release, characterization and antibiofilm activity of these formulations were compared. Characterization of NPs were analyzed by scanning electron microscopy(SEM) and Zetasizer. Drug loading capacity and encapsulation efficiency percentages were measured by spectrophotometric method. PLGA-NPs were spherical in shape with mean sizes of ~300 nm and surface charge of FLZ loaded PVA and PVA-R-PLGA NPs -25,9±1.99, -48,1±2.46, respectively. Sustained release of FLZ (≥60% after 6 h) were obtained in PVA-R PLGA-NPs. The encapsulation efficiency percentages of PVA-FLZ-PLGA and PVA-R-FLZ-PLGA were 50% and %85 respectively. Antibiofilm inhibition percentages are 55% and 63% respectively. These results show that the PVA-R-FLZ-PLGA drug delivery system is a new therapeutic approach that can be used in infections caused by C.albicans.

Evaluation of Nisin-Loaded PLGA Nanoparticles Prepared with Rhamnolipid Cosurfactant against S. aureus Biofilms

“…According to MIC values, N-PVA-R-PLGA NP was effective in reducing the bacterial viability of S. aureus compared to free N. (four times more). These results were found to contain similar results to those reported by da Silva et al (2014) and Üstün and Örtücü (2022) [12,14]. They presented that the antimicrobial properties of bioactive agents loaded PLGA nanoparticles are better than free formulations.…”

“…In addition, in our previous study, PLGA NPs prepared with a PVA-R surfactant, compared to PLGA nanoparticles prepared only with a PVA surfactant, showed superior properties in terms of size, zeta potential and PDI [14].…”

“…The drug delivery system was designed with PVA-rhamnolipid (1:1). The formulation was prepared according to the previous study with some modifications [14]. Briefly, 2.5 mg of purified nisin was used as a drug, and acetone was used as an organic solvent.…”

“…In vitro release test of Üstün and Örtücü (2022) was carried out by making some changes to the protocol according to the dialysis bag technique [14]. For this technique, the dialysis bag was used as MWCO:12 kDa.…”

“…We aim to investigate the effect of PLGA NPs for nisin, prepared using rhamnolipids, which have demonstrated their effective properties in our previous study [14]. Nisin-loaded PLGA NPs were prepared by solvent evaporation method and evaluated in terms of zeta potential, size, percent encapsulation and antimicrobial-antibiofilm activity.…”