Evaluation of the antibacterial effects of aqueous and ethanolic leaf extracts of Aloysia Citriodora (Lemon verbena) on Streptococcus mutans and Streptococcus sobrinus

Shafiee, Faranak; Moghadamnia, Ali Akbar; Shahandeh, Zahra; Sadighian, Farhnaz; Khodadadi, Effat

doi:10.19082/3363

Cited by 11 publications

(7 citation statements)

References 12 publications

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“…The assay was performed in triplicate for each strain, and each set of tests was evaluated independently by a second observer. Subsequently, 143 strains were tested for sensitivity to the selected classes of antibiotics, including the β -lactams (amoxicillin, cefazolin), the macrolides (erythromycin), and the glycopeptide antibiotic (teicoplanin), using a dilution method in a solid phase in the concentration range 0.007–1.5 mg/L [ 20 ]. The statistical significance of differences in susceptibility to selected antibiotics in particular biotypes was determined using the Kruskal-Wallis test complemented by post hoc analysis (Dunn's test).…”

Section: Methodsmentioning

confidence: 99%

Methods of Biotyping of Streptococcus mutans Species with the Routine Test as a Prognostic Value in Early Childhood Caries

Krzyściak

Kościelniak

Papież

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Purpose In order to investigate the suitability of Streptococcus mutans species biotyping by measuring the activity of selected enzymes from a commercial test, criteria were established for biotyping clinical strains from children with dental caries. In addition, the relationships between the selected biotypes, sensitivity to commonly used antibiotics, and early childhood caries were determined. Methods A total of 142 S. mutans isolates from dental plaque of children with caries were divided into different biotypes. Patients were divided into two groups: noncavitated (1-2 in ICDAS) and cavitated (5-6 in ICDAS) lesions. Biotyping criteria were determined based on both the arbitrary method and the clusterization method. The susceptibility of the strains to amoxicillin, cefazolin, erythromycin, and teicoplanin was studied by diluting a solid medium. Results Biotype I was the most common. Mean MIC values showed that the strains belonging to biotypes II and IV were the most sensitive to amoxicillin. For predetermined biotypes, observed differences were dependent on the severity of dental caries. Conclusions The proposed method of S. mutans strains biotyping is relatively quick and simple to use, provided the application of suitable biotyping criteria, and may contribute to the effective prevention of dental caries induced by S. mutans.

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Section: Methodsmentioning

confidence: 99%

Methods of Biotyping of Streptococcus mutans Species with the Routine Test as a Prognostic Value in Early Childhood Caries

Krzyściak

Kościelniak

Papież

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…In the laboratory, samples were immediately cultivated on Columbia blood agar (Merck, Germanycontaining 5% sheep blood) and incubated for 24 to 48 hours at 35˚C, with a CO2 pressure of 5 -10%. Following growth, gram stain slides were prepared from suspected needle sized colonies which displayed α-hemolysis (18). Upon identifying grampositive cocci in diplococci or chain formations, biochemical tests such as catalase (using the identi-fication of 3% hydrogen peroxide), susceptibility to optochin disk and bile solubility test were performed on the colonies.…”

Section: 005)mentioning

confidence: 99%

Antimicrobial susceptibility pattern of oral viridans group Streptococci in children at risk of infective endocarditis

Moudi¹,

Pasdar²,

Babazadeh³

et al. 2021

Acta fac medic Naissensis

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Infective endocarditis (IE) is an important clinical disease in children with a mortality rate of 11.6%. Prophylaxis with antibiotics is one of the most commonly used methods in children at risk of IE; therefore, the evaluation of antibiotic resistance seems necessary in view of its increasing trend. This study aimed to determine the antibiotic susceptibility pattern of oral viridans group streptococci (VGS) isolated from the dental plaque of children at risk of IE. Fifty-one plaque samples were obtained from children aged 3 to 12 years old in the period from April to July 2018. Samples were obtained with sterile swabs and were transferred to the laboratory in Brain Heart Infusion (BHI) Broth. Samples were immediately cultivated on Columbia blood agar. After identifying VGS, antimicrobial susceptibility test (AST) was performed using Mueller-Hinton agar (MHA) with sheep's blood and E-test strips for selected antibiotics. The minimum inhibitory concentration (MIC) was determined for each isolate and the results were reported as sensitive, intermediate and resistant. Fifty-one VGS bacteria were isolated from children with an average age of 7.3 ± 2.5 years. The highest resistance was observed for azithromycin in 36 (70.6%) isolates and then cefazolin in 35 (68.6%) isolates. The highest susceptibility was observed for amoxicillin in 46 (90.2%) isolates. Based on the findings of this study, amoxicillin is the most effective option for prophylaxis in children. Furthermore, cefazolin should be used with caution because bacteria resistant to this antibiotic can transfer resistance genes to other bacteria.

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“…In addition, the medicinal properties of lemon verbena made it an excellent source of herbal medicine for the treatment of various diseases, including spasms, asthma, anxiety, digestive disorders, fever, cold, colic, and insomnia (Bahramsoltani et al, 2018; Bilia et al, 2008; Felgines et al, 2014; Funes et al, 2009). Lemon verbena can boost the immune system and has antibacterial and insecticidal activities (Khani et al, 2012; Shafiee et al, 2016). In fact, the leaves and essential oils (EOs) of this verbena plant are a rich source of phenolic compounds that are mostly responsible for various pharmacological benefits and aromatic values (Carnat et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Melatonin alleviates drought impact on growth and essential oil yield of lemon verbena by enhancing antioxidant responses, mineral balance, and abscisic acid content

Hosseini

Samsampour

Zahedi

et al. 2021

Physiologia Plantarum

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Melatonin has recently emerged as a multifunctional biomolecule with promising aspects in plant stress tolerance. The present study examined the effects of foliar-sprayed melatonin (0, 100, and 200 μM) on growth and essential oil yield attributes of lemon verbena (Lippia citriodora) under water-shortage (mild, moderate and severe). Results revealed that melatonin minimized drought effects on lemon verbena, resulting in improved growth and essential oils yield. Drought impositions gradually and significantly reduced several growth parameters, such as plant height and biomass, whereas melatonin application revived the growth performance of lemon verbena. Melatonin protected the photosynthetic pigments and helped maintain the mineral balance at all levels of drought. Melatonin stimulated the accumulation of proline, soluble sugars and abscisic acid, which were positively correlated with a better preservation of leaf water status in drought-stressed plants. Melatonin also prevented oxidative damages by enhancing the superoxide dismutase, ascorbate peroxidase and catalase activities. Furthermore, increased levels of total phenolic compounds, chicoric acid, caffeic acid and chlorogenic acid, as well as ascorbate and total antioxidant capacity in melatonin-sprayed drought-stressed plants indicated that melatonin helped verbena plants to sustain antioxidant and medicinal properties during drought. Finally, melatonin treatments upheld the concentrations and yield of essential oils in the leaves of lemon verbena regardless of drought severities.These results provided new insights into melatonin-mediated drought tolerance in lemon verbena, and this strategy could be implemented for the successful cultivation of lemon verbena, and perhaps other medicinal plants, in drought-prone areas worldwide.

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Evaluation of the antibacterial effects of aqueous and ethanolic leaf extracts of Aloysia Citriodora (Lemon verbena) on Streptococcus mutans and Streptococcus sobrinus

Cited by 11 publications

References 12 publications

Methods of Biotyping of Streptococcus mutans Species with the Routine Test as a Prognostic Value in Early Childhood Caries

Methods of Biotyping of Streptococcus mutans Species with the Routine Test as a Prognostic Value in Early Childhood Caries

Antimicrobial susceptibility pattern of oral viridans group Streptococci in children at risk of infective endocarditis

Melatonin alleviates drought impact on growth and essential oil yield of lemon verbena by enhancing antioxidant responses, mineral balance, and abscisic acid content

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