Evaluation of the Antibacterial Activity and Toxicity of 2 New Hydrogels: A Pilot Study

Vandenbulcke, Katia; Horvat, Lada-Ivana Laenen; Mil, Martine De; Slegers, Guido; Beele, Hilde

doi:10.1177/1534734606289507

Cited by 17 publications

(8 citation statements)

References 13 publications

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“…The gold standard for targeted local delivery of antimicrobial agents is antibiotic-impregnated bone cement (polymethylmethacrylate-PMMA); other antibiotic-impregnated biodegradable carriers such as sol-gel coatings [9,10] and hydrogels [11,12] are under development. The use of antibiotic loaded PMMA allows delivery of high concentrations of antibiotics to the affected area without causing systemic toxicity.…”

Section: Introductionmentioning

confidence: 99%

The inhibition of Staphylococcus epidermidis biofilm formation by vancomycin-modified titanium alloy and implications for the treatment of periprosthetic infection

et al. 2008

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Peri-prosthetic infections are notoriously difficult to treat as the biomaterial implant is ideal for bacterial adhesion and biofilm formation, resulting in decreased antibiotic sensitivity. Previously, we reported that vancomycin covalently attached to a Ti alloy surface (Vanc-Ti) could prevent bacterial colonization. Herein we examine the effect of this Vanc-Ti surface on Staphylococci epidermidis, a Gram-positive organism prevalent in orthopaedic infections. By direct colony counting and fluorescent visualization of live bacteria, S. epidermidis colonization was significantly inhibited on Vanc-Ti implants. In contrast, the gram-negative organism Escherichia coli readily colonized the Vanc-Ti rod, suggesting retention of antibiotic specificity. By histochemical and SEM analysis, Vanc-Ti prevented S. epidermidis biofilm formation, even in the presence of serum. Furthermore, when challenged multiple times with S. epidermidis, Vanc-Ti rods resisted bacterial colonization. Finally, when S. epidermidis was continuously cultured in the presence of Vanc-Ti, the bacteria maintained a Vanc sensitivity equivalent to the parent strain. These findings indicate that antibiotic derivatization of implants can result in a surface that can resist bacterial colonization. This technology holds great promise for the prevention and treatment of periprosthetic infections. b s t r a c tPeri-prosthetic infections are notoriously difficult to treat as the biomaterial implant is ideal for bacterial adhesion and biofilm formation, resulting in decreased antibiotic sensitivity. Previously, we reported that vancomycin covalently attached to a Ti alloy surface (Vanc-Ti) could prevent bacterial colonization. Herein we examine the effect of this Vanc-Ti surface on Staphylococci epidermidis, a Gram-positive organism prevalent in orthopaedic infections. By direct colony counting and fluorescent visualization of live bacteria, S. epidermidis colonization was significantly inhibited on Vanc-Ti implants. In contrast, the gram-negative organism Escherichia coli readily colonized the Vanc-Ti rod, suggesting retention of antibiotic specificity. By histochemical and SEM analysis, Vanc-Ti prevented S. epidermidis biofilm formation, even in the presence of serum. Furthermore, when challenged multiple times with S. epidermidis, Vanc-Ti rods resisted bacterial colonization. Finally, when S. epidermidis was continuously cultured in the presence of Vanc-Ti, the bacteria maintained a Vanc sensitivity equivalent to the parent strain. These findings indicate that antibiotic derivatization of implants can result in a surface that can resist bacterial colonization. This technology holds great promise for the prevention and treatment of periprosthetic infections.

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Section: Introductionmentioning

confidence: 99%

The inhibition of Staphylococcus epidermidis biofilm formation by vancomycin-modified titanium alloy and implications for the treatment of periprosthetic infection

et al. 2008

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“…Neben den oben genannten In-vitro-Studien, die eine antibakterielle, nichtzytotoxische Wirkung der Enzym-Alginogele belegen [7,33] Der molekulare Wirkmechanismus der Enzym-Alginogele auf den Prozess der Wundheilung ist, wie bereits erläu-tert, nicht vollständig geklärt. Es konnte jedoch in einer vergleichenden klinischen Studie anhand einer Stichprobe gezeigt werden, dass mit Enzym-Alginogelen behandelte Wunden eine deutlich höhere Granulation aufwiesen als Wunden, die mit den gleichen Wundauflagen, jedoch ohne Enzym-Alginogele behandelt wurden [16,25].…”

Section: Klinische Studiendatenunclassified

Wundbehandlung mit Enzym-Alginogelen

et al. 2016

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“…Pre-clinical data by Vandenbulcke et al and de Smet et al showed that Flaminal® Forte is not toxic to keratinocytes and fibroblasts in vitro [ 15 , 27 ]. In effect, Flaminal® Forte may not damage skin cells which will finally result in undelayed wound healing.…”

Section: Introductionmentioning

confidence: 99%

Clinical effectiveness, quality of life and cost-effectiveness of Flaminal® versus Flamazine® in the treatment of partial thickness burns: study protocol for a randomized controlled trial

Rashaan

Krijnen

Marle

et al. 2016

Trials

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BackgroundPartial thickness burns are painful, difficult to manage and can have a negative effect on quality of life through scarring, permanent disfigurement and loss of function. The aim of burn treatment in partial thickness burns is to save lives, stimulate wound healing by creating an optimumly moist wound environment, to have debriding and analgesic effects, protect the wound from infection and be convenient for the patient and caregivers. However, there is no consensus on the optimal treatment of partial thickness wounds. Flaminal® and Flamazine® are two standard treatment options that provide the above mentioned properties in burn treatment. Nevertheless, no randomized controlled study has yet compared these two common treatment modalities in partial thickness burns. Thus, the aim of this study is to evaluate the clinical effectiveness, quality of life and cost-effectiveness of Flaminal® versus Flamazine® in the treatment of partial thickness burns.Methods/DesignIn this two-arm open multi-center randomized controlled trial, 90 patients will be randomized between Flaminal® and Flamazine® and followed for 12 months. The study population will consist of competent or temporarily non-competent (because of sedation and/or intubation) patients, 18 years of age or older, with acute partial thickness burns and a total body surface area (TBSA) of less than 30 %. The main study outcome is time to complete re-epithelialization (greater than 95 %). Secondary outcome measures include need for grafting, wound colonization/infection, number of dressing changes, pain and anxiety, scar formation, health-related quality of life (HRQoL), and costs.DiscussionThis study will contribute to the optimal treatment of patients with partial thickness burn wounds and will provide evidence on the (cost-)effectiveness and quality of life of Flaminal® versus Flamazine® in the treatment of partial thickness burns.Trial registrationNetherlands Trial Register NTR4486, registered on 2 April 2014.

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Evaluation of the Antibacterial Activity and Toxicity of 2 New Hydrogels: A Pilot Study

Cited by 17 publications

References 13 publications

The inhibition of Staphylococcus epidermidis biofilm formation by vancomycin-modified titanium alloy and implications for the treatment of periprosthetic infection

The inhibition of Staphylococcus epidermidis biofilm formation by vancomycin-modified titanium alloy and implications for the treatment of periprosthetic infection

Wundbehandlung mit Enzym-Alginogelen

Clinical effectiveness, quality of life and cost-effectiveness of Flaminal® versus Flamazine® in the treatment of partial thickness burns: study protocol for a randomized controlled trial

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