Evaluation of the Antibacterial Activity of Essential Oil of Dysphania ambrosioides (L.) Mosyakin and Clemants Against Clinical Multidrug-Resistant Bacteria

Azghar, Ali; Dalli, Mohammed; Loukili, El Hassania; Belbachir, Yousra; Tahri, Maroua; Benaissa, Elmostapha; Lahlou, Yassine Ben; Elouennass, Mostafa; Maleb, Adil

doi:10.3923/ajps.2023.75.81

Cited by 3 publications

(1 citation statement)

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“…The essential oils of three species (S. officinalis, O. compactum, and S. aromaticum) (Figure 10) were extracted with hydrodistillation using Clevenger-apparatus equipment, as described by Azghar, A. et al [60]. The three fresh species (150 g) were accurately weighed and combined with 500 ml of purified water.…”

Section: Hydrodistillation Protocolmentioning

confidence: 99%

Phytochemical Composition and Pharmacological Activities of Three Essential Oils Collected from Eastern Morocco (Origanum compactum, Salvia officinalis, and Syzygium aromaticum): A Comparative Study

Loukili,

Ouahabi,

Elbouzidi

et al. 2023

Plants

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Throughout history, essential oils have been employed for their pleasing scents and potential therapeutic benefits. These oils have shown promise in various areas, including aromatherapy, personal care products, natural remedies, and even as alternatives to traditional cleaning agents or pest control solutions. The study aimed to explore the chemical makeup, antioxidant, and antibacterial properties of Origanum compactum Benth., Salvia officinalis L., and Syzygium aromaticum (L.) Merr. et Perry. Initially, the composition of the three essential oils, O. compactum (HO), S. officinalis (HS), and S. aromaticum (HC) was analyzed using GC-MS technology, revealing significant differences in the identified compounds. α-thujone emerged as the predominant volatile component in the oils, making up 78.04% of the composition, followed by eugenol, which constituted 72.66% and 11.22% of the HC and HO oils, respectively. To gauge antioxidant capabilities, tests involving DPPH scavenging capacity and total antioxidant capacity were conducted. Antioxidant activity was determined through the phosphomolybdate test and the DPPH• radical scavenging activity, with the HO essential oil displaying significant scavenging capacity (IC50 of 0.12 ± 0.02 mg/mL), similar to ascorbic acid (IC50 of 0.26 ± 0.24 mg/mL). Similarly, the TAC assay for HO oil revealed an IC50 of 1086.81 ± 0.32 µM AAE/mg. Additionally, the oils’ effectiveness against four bacterial strains, namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Listeria monocytogenes, and five fungi, Geotrichum candidum, Aspergillus niger, Saccharomyces cerevisiae, Candida glabrata, and Candida albicans, was tested in vitro. The examined essential oils generally exhibited limited antimicrobial effects, with the exception of HC oil, which demonstrated an exceptionally impressive level of antifungal activity. In order to clarify the antioxidant, antibacterial, and antifungal effects of the identified plant compounds, we employed computational methods, specifically molecular docking. This technique involved studying the interactions between these compounds and established protein targets associated with antioxidant, antibacterial, and antifungal activities.

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Section: Hydrodistillation Protocolmentioning

confidence: 99%