Evaluation of the Anti-Leishmania Major Activity of Satureja Bakhtiarica Essential Oil in Vitro

Abstract

Leishmaniasis is a painless chronic skin disease that is caused by the protozoan parasite Leishmania. Due to the importance of this disease and the side effects of chemical drugs, use of drugs of plant origin to treat Leishmaniasis is very important. In the present study, the chemical composition and the anti-Leishmania major activity of the essential oils obtained from Satureja bakhtiarica were evaluated in vitro. The oils were extracted using a Clevenger apparatus and then the chemical composition was analyzed by GC-MS. Promastigotes of L. major were cultured in both N.N.N and RPMI1640 media. GC-MS analysis showed 13 compounds, in which the major components were the phenolic (37.4%) compounds, thymol (22.6%) and p-cymene (19.3%). The essential oil of S. bakhtiarica showed higher activity against L. major than the standard anti-Leishmania drug, glucantime,. Perhaps because of the high concentration of phenolic compounds in the essential oil, all the parasites were killed after 24 hours. The essential oil from S. bakhtiarica is a potential plant drug against leishmaniasis. Further studies are necessary to evaluate this oil in animal models (in vivo) for future drug applications.

Designing, Optimization and Characterization of Trifluralin Transfersomal Gel to Passively Target Cutaneous Leishmaniasis

“…In later case, IC50 of TFL-TFS was determined to be 2.86-folds reduction in contrast to TFLsolution. However, no inhibitory effect of DMSO 1% was in accordance with already published report (Mohammadpour et al, 2012).…”

Antileishmanial Activity of the Essential Oil fromBixa orellana

“…Previously, we have shown the in vitro and in vivo potentialities of hydroalcoholic extracts from seeds of B. orellana against Leishmania amazonensis (Garcia et al, 2011). However, in the last decades, there has been a resurgence of interest in EOs, which have demonstrated antileishmanial potentialities (Ahmed et al, 2011;Machado et al, 2012;Mohammadpour et al, 2012;Monzote et al, 2012). The aim of the present study was to determinate the chemical composition and evaluate the antileishmanial activity of EO from seeds of B. orellana.…”

Anti-Trypanosomatidae Activity of Essential Oils and Their Main Components from Selected Medicinal Plants

“…Satureja montana EO has interesting properties against bacteria, fungi, viruses, helminths, insects, and protozoa [ 24 , 46 , 47 , 48 , 49 ]. EOs from Satureja khuzestanica , Satureja bakhtiarica , and Satureja punctata demonstrated antileishmanial effects on L. major , L. donovani , and L. aethiopica previously [ 50 , 51 , 52 ]. S. montana EO (HD and SD) was active against both parasites, with P. davidi being more sensitive.…”