Evaluation of the anti-hyperglycemic effect and safety of microorganism 1-deoxynojirimycin

Abstract

1-Deoxynojirimycin (DNJ) is a potent α-glucosidase inhibitor and thus beneficial for prevention of diabetes. While we have succeeded in obtaining the culture supernatant extract (CSE) rich in DNJ from microorganism source, information regarding its anti-hyperglycemic effect and safety were still limited. Therefore, this study was aimed to evaluate the anti-hyperglycemic effect and safety of microorganism DNJ. Oral sucrose tolerance test was performed, and the result showed that CSE was able to significantly suppress the blood glucose elevation and suggested DNJ as the main active compound. To determine its safety, the absorption and excretion of microorganism DNJ were evaluated using 15N labeling method. Our findings investigated the recovery rate of 15N from DNJ reached 80% up to 48 hours after oral administration, suggesting its rapid excretion, suggesting the safety of DNJ. This study verified the functional properties and safety of DNJ from microorganisms, suggesting its potential use for functional purpose.

Physiological Effects and Organ Distribution of <i>Bacillus amyloliquefaciens</i> AS385 Culture Broth Powder Containing 1-Deoxynojirimycin in C57BL/6J Mice

“…. CBP was prepared according to our previous study (19) with modifications. First, B. amyloliquefaciens AS385 was cultured in 100 mL of medium containing 4% soybean peptone and 5% sorbitol.…”

“…Previously, we discovered that B. amyloliquefaciens AS385, a bacterial strain that is commonly found in fermented food products, can produce high amount of DNJ after being cultivated in the culture medium supplemented with sorbitol (12). Next, we developed the purification method for B. amyloliquefaciens AS385 culture medium to increase the recovery rate of microbial DNJ (19) by using ethanol precipitation followed by cation exchange absorption-desorption. However, considering the amount of time and cost that are required for this method, simpler method is more preferable for large-scale production.…”

“…Moreover, while numerous studies have reported the beneficial effects of DNJ (3,(5)(6)(7)(8)(15)(16)(17)(18)(19)(20)(21)(22), the information about its absorption and distribution in organs after daily consumption in diet was lacking. Understanding the distribution of DNJ in organs is crucial and may serve as a basis to find the underlying mechanism on how DNJ exerts its beneficial effects in the body.…”

Differential Effects of Red Yeast Rice, Berberis Aristata and Morus Alba Extracts, Active Components of LopiGLIK&reg;, on PCSK9 in HepG2 Cells

“…When glucose tolerance evaluations are carried out, these are characterized by improved glycemia and a markedly reduced insulinemic response. The α-glycosidase inhibitory activity appears to be superior to that of acarbose and potentially this treatment may reduce the development of diabetes in experimental animals, thus suggesting the application in clinical condition [35].…”

Transglycosylation Properties of a Novelα-1,4-Glucanotransferase fromBacteroides thetaiotaomicronand Its Application in Developing anα-Glucosidase-Specific Inhibitor

“…1-Deoxynojirimycin (DNJ), an aza-sugar, has structural characteristics similar to those of cyclic monosaccharides; however, the oxygen is substituted with a nitrogen atom. DNJ prevents glucose from entering the bloodstream from the intestines by inhibiting the activities of α-amylase and α-glucosidase [11,12]. Intestinal α-glucosidase is one of the glucosidases of the small intestinal epithelium [13].…”