Evaluation of the anti-angiogenic effect of aloe-emodin

Abstract

The present study identified aloe-emodin (AE, a hydroxyanthraquinone from Aloe vera and other plants) as a new anti-angiogenic compound with inhibitory effects in an in vivo angiogenesis assay and evaluates its effects on specific key steps of the angiogenic process. AE inhibits endothelial cell proliferation, but this effect is not cell specific, since AE also inhibits tumor cell proliferation. Cell migration and invasion are not remarkably affected by AE. On the other hand, AE has different effects on endothelial and tumor cell gelatinases. Two main targets of the pharmacological action of AE as an anti-angiogenic compound seem to be urokinase secretion and tubule formation of endothelial cells. Finally, AE produces a remarkable photocytotoxic effect on tumor cells. Taken together, our data indicate that AE can behave both as an anti-tumor and an anti-angiogenic compound and suggest that AE could be a candidate drug for photodynamic therapy.

Evaluation of Phytoconstituents of Bryonopsis laciniosa fruit by UV-Visible Spectroscopy and FTIR analysis

“…They are anti apoptotic, anti aging, anti carcinogenic, anti inflammatory, anti atherosclerotic, cardiovascular protective and prevent from oxidative stress as well as inhibition of angiogenesis and cell proliferation. 26 Emodins has anti angiogenetic effect 27 Plenty of pharmaceutical studies indicates that emodins have anti inflammatory, anticancer and antimicrobial activity anti-cancer, anti-microbial and antiinflammatory effects. 28 The UV-Visible spectra were performed to identify the compounds containing σ-bonds, π-bonds, and lone pair of electrons, chromophores and aromatic rings The profile showed the peaks at 208.04, 271.94 and 881.96 nm with the absorption 2.76,0.72 and 0.04 respectively Table 3 and Figure 1.…”

Aloe-emodin triggers ROS and Ca 2+ production and decreases the levels of mitochondrial membrane potential of human brain capillary endothelial cells

“…Data showed that AE inhibits cell proliferation and in vitro cell migration/invasion and tube formation of human umbilical vein endothelial cells (HUVECs) [11]. The ability of AE treatment to inhibit the proliferation of primary bovine aortic endothelial cells (BAEC) have been also proven [10]. Other anthroquinone derivatives, rhein [7] and emodin [13], also possess anti-angiogenic activity, inhibiting vessel formation in zebra fish embryos, HUVEC proliferation, migration and tube formation as well as vessel formation in chick embryos and proliferation of BAEC cells, respectively.…”

“…Hundreds of natural compounds of plant and animal origin have properties of angiogenic inhibitors [6-. One of them is aloe-emodin [1,8-dihydroxy-3-hydroxymethylanthraquinone], bioactive hydroxyanthraquinone Aloe barbadensis Miller (Aloe vera), Rheum officinale, as well as in leaves and roots of other plants [9], showing in vitro and antitumor activity and anti-angiogenic properties [10,11].…”

“…Notably, studies validating the modulating role of AE in the regulation of angiogenesis and metastasis are just a few [10,12] and very little is known about the mechanisms via which AE inhibits the proliferation of endothelial cells. AE can influence all key steps involved in the angiogenesis, including the local proliferation of endothelial cells, migration, invasion and finally differentiation and new vessels formation.…”

“…Recently, the antiproliferative effect of AE on human breast adenocarcinoma cell line MCF-7, associated with intracellular Ca 2+ signaling, was found [15]. However, although data indicate that AE behaves both as an antitumor and an anti-angiogenic compound [10], from the best of our knowledge, there are no studies about related processes into endothelial cells, nor about the effect of AE on human brain capillary endothelial cells, in particular. In order to get inside the molecular mechanisms by which AE affect endothelial cell proliferation, we studied its dose-dependent cytotoxic effect on brain micro vascular endothelial cells.…”