“…In contrast, both the two-step systems, 10-10 and Oxysept 1, were cysticidal, resulting in at least a 3-log kill after 4 h of contact time and complete kill after 6 h. This observation is in accord with previous studies for two-step systems (5,13,15). As expected, the hydrogen peroxide solutions used in the onestep systems were also cysticidal.…”
Section: Discussionsupporting
confidence: 77%
“…This study has confirmed the efficacy of two-step commercial hydrogen peroxide contact lens disinfection systems against Acanthamoeba cysts providing a contact time of at least 4 h is used before neutralization (2,5,15). The potential disadvantage of hydrogen peroxide disinfection is that the lenses cannot be stored in the solution (25).…”
Section: Discussionmentioning
confidence: 53%
“…This requirement is not met by one-step systems that rapidly neutralize the peroxide resulting in no cysticidal activity (5,15,25). The possible exception was found with use of the AOSept 1-Step system, which resulted in a 1.28 Ϯ 0.41-log reduction after the manufacturer's recommended contact time of 6 h, compared with a Ͻ1-log reduction for the other systems.…”
Section: Discussionmentioning
confidence: 99%
“…Consequently, a variety of strains, methods for cyst preparation, and assay protocols have been used (2,3,5,9,15,25,26). This has frequently led to conflicting reports on the efficacy of contact lens disinfectants, including hydrogen peroxide (2,18,26).…”
Section: Discussionmentioning
confidence: 99%
“…Hydrogen peroxide is an effective microbial disinfectant, destroying pathogens by oxidation (11). It is active against the resistant cyst form of Acanthamoeba when used at a concentration of 3% with an exposure time of at least 4 to 6 h (5,14). However, hydrogen peroxide is toxic to the cornea and must be neutralized before lens wear to avoid pronounced stinging, lacrimation, hyperemia, and possible corneal damage (7,8).…”
Acanthamoeba is a free-living amoeba causing a potentially blinding infection of the cornea. Contact lens wearers are most at risk and account for some 95% of cases. Hydrogen peroxide is used for contact lens disinfection due to its broad antimicrobial activity. Lenses must be neutralized before use to avoid pronounced stinging and possible corneal damage. Neutralization is achieved by adding a catalyst during the disinfection process (one-step) or afterwards (two-step). Here, the activities of commercial peroxide systems and individual solutions against trophozoites and cysts of Acanthamoeba polyphaga were compared. All disinfection systems were active against trophozoites, giving a >3-log (99.9%) kill within 1 h. Of the four one-step systems, only one showed some cysticidal activity, giving a 1.28 ؎ 0.41-log reduction. Both two-step systems were cysticidal, giving a >3-log kill at 4 h. All system peroxide solutions were cysticidal, giving a >3-log kill by 4 to 6 h. Variation in the cysticidal rate was observed with two solutions that gave a 1.8-to 2.1-log kill at 4 h compared with 3.0 to 4.0 for the rest (P < 0.05). No cysticidal activity was found with the peroxigen sodium perborate or the contact lens protein remover subtilisin A. Two-step systems are cysticidal providing contact times of at least 4 h are employed. Variation in cyst killing occurs between peroxide solutions, possibly due to formulation differences. One-step systems are less effective against Acanthamoeba cysts due to rapid peroxide neutralization. The cysticidal activity of one-step systems could be improved if neutralization rates were retarded.
“…In contrast, both the two-step systems, 10-10 and Oxysept 1, were cysticidal, resulting in at least a 3-log kill after 4 h of contact time and complete kill after 6 h. This observation is in accord with previous studies for two-step systems (5,13,15). As expected, the hydrogen peroxide solutions used in the onestep systems were also cysticidal.…”
Section: Discussionsupporting
confidence: 77%
“…This study has confirmed the efficacy of two-step commercial hydrogen peroxide contact lens disinfection systems against Acanthamoeba cysts providing a contact time of at least 4 h is used before neutralization (2,5,15). The potential disadvantage of hydrogen peroxide disinfection is that the lenses cannot be stored in the solution (25).…”
Section: Discussionmentioning
confidence: 53%
“…This requirement is not met by one-step systems that rapidly neutralize the peroxide resulting in no cysticidal activity (5,15,25). The possible exception was found with use of the AOSept 1-Step system, which resulted in a 1.28 Ϯ 0.41-log reduction after the manufacturer's recommended contact time of 6 h, compared with a Ͻ1-log reduction for the other systems.…”
Section: Discussionmentioning
confidence: 99%
“…Consequently, a variety of strains, methods for cyst preparation, and assay protocols have been used (2,3,5,9,15,25,26). This has frequently led to conflicting reports on the efficacy of contact lens disinfectants, including hydrogen peroxide (2,18,26).…”
Section: Discussionmentioning
confidence: 99%
“…Hydrogen peroxide is an effective microbial disinfectant, destroying pathogens by oxidation (11). It is active against the resistant cyst form of Acanthamoeba when used at a concentration of 3% with an exposure time of at least 4 to 6 h (5,14). However, hydrogen peroxide is toxic to the cornea and must be neutralized before lens wear to avoid pronounced stinging, lacrimation, hyperemia, and possible corneal damage (7,8).…”
Acanthamoeba is a free-living amoeba causing a potentially blinding infection of the cornea. Contact lens wearers are most at risk and account for some 95% of cases. Hydrogen peroxide is used for contact lens disinfection due to its broad antimicrobial activity. Lenses must be neutralized before use to avoid pronounced stinging and possible corneal damage. Neutralization is achieved by adding a catalyst during the disinfection process (one-step) or afterwards (two-step). Here, the activities of commercial peroxide systems and individual solutions against trophozoites and cysts of Acanthamoeba polyphaga were compared. All disinfection systems were active against trophozoites, giving a >3-log (99.9%) kill within 1 h. Of the four one-step systems, only one showed some cysticidal activity, giving a 1.28 ؎ 0.41-log reduction. Both two-step systems were cysticidal, giving a >3-log kill at 4 h. All system peroxide solutions were cysticidal, giving a >3-log kill by 4 to 6 h. Variation in the cysticidal rate was observed with two solutions that gave a 1.8-to 2.1-log kill at 4 h compared with 3.0 to 4.0 for the rest (P < 0.05). No cysticidal activity was found with the peroxigen sodium perborate or the contact lens protein remover subtilisin A. Two-step systems are cysticidal providing contact times of at least 4 h are employed. Variation in cyst killing occurs between peroxide solutions, possibly due to formulation differences. One-step systems are less effective against Acanthamoeba cysts due to rapid peroxide neutralization. The cysticidal activity of one-step systems could be improved if neutralization rates were retarded.
Alkaliphiles are interesting groups of extremophilic organisms that thrive at pH of 9.0 and above. Many of their products, in particular enzymes, have found widespread applications in industry, primarily in the detergent and laundry industries. While the enzymes have been a runaway success from the industrial point of view, many more products have been reported from alkaliphiles such as antibiotics and carotenoids. Less known are their potential for degradation of xenobiotics. They also play a key role in biogeocycling of important inorganic compounds. This review provides an insight into the huge diversity of alkaliphilic bacteria, the varied products obtained from them, and the need for further investigations on these interesting bacteria.
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