Evaluation of the annual killifish Nothobranchius guentheri as a tool for rapid acute toxicity screening

Shedd, Tommy R.; Widder, Mark W.; Toussaint, Margaret W.; Sunkel, Mark C.; Hull, Eugene

doi:10.1002/etc.5620181020

Cited by 21 publications

(14 citation statements)

References 16 publications

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“…The species C. melanotaenia was evaluated in 18 tests with copper sulfate (CuSO 4 ·5H 2 O), in which the mean 96-h EC50 was 0.09 mg/L and ranged from 0.05 to 0.13 mg/L ( Table 1). The mean sensitivity of C. melanotaenia to CuSO 4 ·5H 2 O in this study bracketed the documented values for Pimephales promelas, which were 0.025 mg/L [11] and 0.48 mg/L [12] ( Table 2). Cynopoecilus melanotaenia showed higher sensitivity when compared to the data presented by Bertoletti et al [13] for Danio rerio (0.57 mg/L) or when compared to the results obtained for Salmo gairdnerii (0.30 mg/L) [14], Lepomis macrochirus (0.66 mg/L) [11], and Oreochromis mossambicus (2.78 mg/L) [15].…”

Section: Resultssupporting

confidence: 85%

Assessment of the freshwater annual fish Cynopoecilus melanotaenia as a toxicity test organism using three reference substances

Arenzon

Pinto

Colombo

et al. 2003

Enviro Toxic and Chemistry

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This study presents a preliminary evaluation of the use of the Brazilian fish Cynopoecilus melanotaenia as a test organism in toxicity tests. The cryptobiotic stage presented by the eggs of fish C. melanotaenia can overcome the difficulty of continuously keeping cultures and recruiting healthy animals in sufficient numbers to be used in toxicity tests. In order to determine the applicability of this species as a test organism, three different reference substances were evaluated in 96-h acute toxicity tests: Copper sulfate (CuSO4 x 5H2O), sodium dodecil sulfate (C12H25NaO4S), and sodium chloride (NaCl). Sensitivity ranged as follows: copper sulfate (0.05-0.13 mg/L), sodium dodecil sulfate (10.7-19.0 mg/L), and sodium chloride (1.44-1.96 g/L). We conclude that C. melanotaenia shows potential as a test organism in toxicity tests; however, further research should be conducted with other substances and should be compared with the research on other species before we can reach more conclusive results.

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Section: Resultssupporting

confidence: 85%

Assessment of the freshwater annual fish Cynopoecilus melanotaenia as a toxicity test organism using three reference substances

Arenzon

Pinto

Colombo

et al. 2003

Enviro Toxic and Chemistry

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“…The genus Nothobranchius has great potential for experimental applications in developmental biology (Van Haarlem, 1979), toxicology (Shedd et al , 1999) and evolutionary biology (Haas, 1976; Reichard & Polačik, 2010). As early as the 1970s, Nothobranchius guentheri (Pfeffer) was recognized as a promising model organism for the study of ageing processes due to its short maximum life span of c. 15 months (Markofsky & Perlmutter, 1973).…”

Section: Introductionmentioning

confidence: 99%

Age structure of annual Nothobranchius fishes in Mozambique: is there a hatching synchrony?

Polačik

Donner

Reichard

2011

Journal of Fish Biology

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The age structures of populations of African annual Nothobranchius spp. were examined for the first time. Daily increments in sagittal otoliths of Nothobranchiusjurzeri, Nothobranchius kadled, No/hobranch ius orthollotus and Nothobranchius rachovii from southern and central Mozambique were used for age determination. Four hypotheses were tested: (I) timing of hatching is consistent with the calendar onset of the rainy season, (2) hatching is synchronized within a population in a pool , (3) there is a difference in hatching date between geographical regions differing in mean total annual precipitation and (4) sympatric Nothobranchius spp. hatch at the same time. The results show that daily increment analysis represents an applicable method for age determination in Nothobrall chius spp. Despite a significant positive relationship between age and size of fishes, a pronounced variation in fish size at an age precluded the use of fish size as a valid age marker. Timing of hatchi'ng was 110t consistent with the calendar onset of the rainy season. Interpopulation variability was observed in the degree of hatching date synchronization within a population. Hatching dates were relatively uniform in some populations, while there was considerable variability in others. Differences in timing of hatching date were found in on ly I of 2 years within the three regions investigated (Chefu, lower Limpopo and Sofala regions), each of which differed in mean total annual rainfall. The hatching dates of sympatric Nothobranchius spp. were marginally different, but further testing on a larger sample is needed for conclusive results.

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“…In toxicological research, Nothobranchius spp. were recognized as a useful tool for acute toxicity tests due to their sensitivity to toxic compounds and ability to be maintained at the diapause stage for prolonged periods without laboratory testing (Shedd et al , 1999). Recently, Nothobranchius spp.…”

Section: Introductionmentioning

confidence: 99%

Diet overlap among three sympatric African annual killifish species Nothobranchius spp. from Mozambique

Polačik

Reichard

2010

Journal of Fish Biology

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The diet patterns of three Nothobranchius species (N. furzeri, N. orthonotus and N. rachovii), small, short-lived annual killifish from temporary pools in African savannah were investigated. Four sites with contrasting fish density and water surface area were sampled in 2008 and 2009 in southern Mozambique. Stomach content analysis showed that all the species examined were generalists, with diets largely based on aquatic invertebrates. The same invertebrate prey categories were consumed by all three species, but their relative proportions varied across species. The largest species, N. orthonotus, showed the most distinct diet and consumed vertebrates (juvenile lungfish Protopterus annectens and larval Amphibia) and a relatively high proportion of Odonata, Coleoptera and Ephemeroptera larvae. The diet of the other two species (N. furzeri and N. rachovii) showed a stronger overlap, did not include vertebrates, but was rich in small crustaceans (Cladocera, Copepoda, Ostracoda and Conchostraca). Mosquito (Diptera) larvae formed only a negligible part of the diet of all the three species.

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Evaluation of the annual killifish Nothobranchius guentheri as a tool for rapid acute toxicity screening

Cited by 21 publications

References 16 publications

Assessment of the freshwater annual fish Cynopoecilus melanotaenia as a toxicity test organism using three reference substances

Assessment of the freshwater annual fish Cynopoecilus melanotaenia as a toxicity test organism using three reference substances

Age structure of annual Nothobranchius fishes in Mozambique: is there a hatching synchrony?

Diet overlap among three sympatric African annual killifish species Nothobranchius spp. from Mozambique

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