Evaluation of the Allergenicity Potential of TcPR-10 Protein from Theobroma cacao

Menezes, Sara Pereira; Santos, Jane Lima dos; Cardoso, Thyago Hermylly Santana; Pirovani, Carlos Priminho; Micheli, Fabienne; Noronha, Fátima Soares Noronha; Alves, Andréa C.; Faria, Ana Maria Caetano; Gesteira, Abelmon da Silva

doi:10.1371/journal.pone.0037969

Cited by 9 publications

(5 citation statements)

References 61 publications

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“…With regard to the hosts, 10 Theobroma cacao proteins of biotechnological interest have been characterized in vitro: four cystatins [ 117 ], one TcPR-10 [ 118 ], one β-1,3-1,4-Glucanase (TcGlu2) [ 119 ], one PR-4b [ 120 ], and one osmotin [ 121 ]. All of them have been shown to have a fungicidal effect on M. perniciosa .…”

Section: Discussionmentioning

confidence: 99%

State of the Art of the Molecular Biology of the Interaction between Cocoa and Witches’ Broom Disease: A Systematic Review

Santos

Mora-Ocampo

Novais

et al. 2023

IJMS

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Significant scientific advances to elucidate the Moniliophthora perniciosa pathosystem have been achieved in recent years, but the molecular biology of this pathogen-host interaction is still a field with many unanswered questions. In order to present insights at the molecular level, we present the first systematic review on the theme. All told, 1118 studies were extracted from public databases. Of these, 109 were eligible for the review, based on the inclusion and exclusion criteria. The results indicated that understanding the transition from the biotrophic-necrotrophic phase of the fungus is crucial for control of the disease. Proteins with strong biotechnological potential or that can be targets for pathosystem intervention were identified, but studies regarding possible applications are still limited. The studies identified revealed important genes in the M. perniciosa-host interaction and efficient molecular markers in the search for genetic variability and sources of resistance, with Theobroma cacao being the most common host. An arsenal of effectors already identified and not explored in the pathosystem were highlighted. This systematic review contributes to the understanding of the pathosystem at the molecular level, offering new insights and proposing different paths for the development of new strategies to control witches’ broom disease.

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Section: Discussionmentioning

confidence: 99%

State of the Art of the Molecular Biology of the Interaction between Cocoa and Witches’ Broom Disease: A Systematic Review

Santos

Mora-Ocampo

Novais

et al. 2023

IJMS

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“…Typical allergy symptoms are usually triggered by the production of immunoglobulin E (IgE) [32,44]. In this work, rats were used as an animal model to examine the accumulation of IgE [16,45] after sensitization to non-transgenic and transgenic sugarcane juice.…”

Section: In Vivo Allergenicity Assay In Rats and Measurement Of The Pmentioning

confidence: 99%

Development of Allergenicity and Toxicity Assessment Methods for Evaluating Transgenic Sugarcane Overexpressing Sucrose–Phosphate Synthase

et al. 2019

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Sugarcane is considered as an industrial crop that produces sugar. The number of transgenic sugarcane on the market is currently increasing. Therefore, investigation of the potential allergens and toxics in transgenic sugarcane is necessary, since there is less information regarding food safety for human consumption. Bioinformatics and experimental analysis were used for the validation of the allergenic potential of transgenic sugarcane overexpressing sucrose–phosphate synthase (SPS). Bioinformatics analysis showed that SPS has no homology with any known allergenic proteins. However, eight-residues identical contiguous sequence was detected, and further specific assessment is required to confirm the potential of allergenicity. The results of protein stability evaluation showed that SPS gradually decreased at 28 °C and rapidly inactivated at 60 °C and 90 °C by heat treatment. In addition, total protein was degraded by simulated gastric fluids (SGF), and simulated intestine fluid (SIF) assays for one-minute incubation. The level of specific IgE in the transgenic sugarcane and controls also showed no potential risk of allergy. An acute oral toxicity assay was performed by oral gavage of transgenic sugarcane juice in mice. The LD50 for transgenic sugarcane juice was >25 gr/kg body weight. We propose a development method for allergenicity and toxicity assessment in transgenic sugarcane.

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“…The lysate was cleared by centrifugation for 15 min at 10,000×g and then applied to a Ni-NTA-agarose column (Superflow Cartridge,QIAGEN) in buffer A . The recombinant PhzO was purified to homogeneity as previously described [24] .…”

Section: Methodsmentioning

confidence: 99%

Reaction Kinetics for the Biocatalytic Conversion of Phenazine-1-Carboxylic Acid to 2-Hydroxyphenazine

et al. 2014

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The phenazine derivative 2-hydroxyphenazine (2-OH-PHZ) plays an important role in the biocontrol of plant diseases, and exhibits stronger bacteriostatic and fungistatic activity than phenazine-1-carboxylic acid (PCA) toward some pathogens. PhzO has been shown to be responsible for the conversion of PCA to 2-OH-PHZ, however the kinetics of the reaction have not been systematically studied. Further, the yield of 2-OH-PHZ in fermentation culture is quite low and enhancement in our understanding of the reaction kinetics may contribute to improvements in large-scale, high-yield production of 2-OH-PHZ for biological control and other applications. In this study we confirmed previous reports that free PCA is converted to 2-hydroxy-phenazine-1-carboxylic acid (2-OH-PCA) by the action of a single enzyme PhzO, and particularly demonstrate that this reaction is dependent on NADP(H) and Fe3+. Fe3+ enhanced the conversion from PCA to 2-OH-PHZ and 28°C was a optimum temperature for the conversion. However, PCA added in excess to the culture inhibited the production of 2-OH-PHZ. 2-OH-PCA was extracted and purified from the broth, and it was confirmed that the decarboxylation of 2-OH-PCA could occur without the involvement of any enzyme. A kinetic analysis of the conversion of 2-OH-PCA to 2-OH-PHZ in the absence of enzyme and under different temperatures and pHs in vitro, revealed that the conversion followed first-order reaction kinetics. In the fermentation, the concentration of 2-OH-PCA increased to about 90 mg/L within a red precipitate fraction, as compared to 37 mg/L within the supernatant. The results of this study elucidate the reaction kinetics involved in the biosynthesis of 2-OH-PHZ and provide insights into in vitro methods to enhance yields of 2-OH-PHZ.

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Evaluation of the Allergenicity Potential of TcPR-10 Protein from Theobroma cacao

Cited by 9 publications

References 61 publications

State of the Art of the Molecular Biology of the Interaction between Cocoa and Witches’ Broom Disease: A Systematic Review

State of the Art of the Molecular Biology of the Interaction between Cocoa and Witches’ Broom Disease: A Systematic Review

Development of Allergenicity and Toxicity Assessment Methods for Evaluating Transgenic Sugarcane Overexpressing Sucrose–Phosphate Synthase

Reaction Kinetics for the Biocatalytic Conversion of Phenazine-1-Carboxylic Acid to 2-Hydroxyphenazine

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