Evaluation of the allele-sharing approach, known as the IBS method, in kinship analysis

Tamura, Tomonori; Osawa, Motoki; Kimura, Ryosuke; Inaoka, Yoshihiko; Tanaka, S.; Satoh, Fumiko; Sato, Itaru

doi:10.1016/j.jflm.2012.05.005

Cited by 14 publications

(13 citation statements)

References 22 publications

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“…However, Tamaki et al previously found that this kit occasionally has limitations when obtaining a significant LR in pairwise sibship testing [8,9]. This limitation can be overcome by analyzing additional autosomal STR loci [10].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of advanced multiplex short tandem repeat systems in pairwise kinship analysis

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Evaluation of advanced multiplex short tandem repeat systems in pairwise kinship analysis

et al. 2015

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“…DNA from unrelated individuals was used to ensure the linkage of SNPs. Buccal swabs were obtained from 52 Japanese individuals for DNA extraction, as described previously [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Allelic Imbalance of mRNA Associated withα2-HS Glycoprotein (Fetuin-A) Polymorphism

et al. 2015

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Alpha 2-HS glycoprotein (AHSG), also designated as fetuin-A, exhibits polymorphism in population genetics consisting of two major alleles of AHSG ∗ 1 and AHSG ∗ 2. The serum level in the AHSG ∗ 1 homozygote is significantly higher than that of the AHSG ∗ 2 homozygote. This study examined the molecular mechanism for the cis-regulatory expression. To quantitate allele-specific mRNA in intra-assays of the heterozygote, RT-PCR method employing primers that were incorporated to the two closely located SNPs was developed. The respective magnitudes of AHSG ∗ 1 to AHSG ∗ 2 in the liver tissues and hepatic culture cells of PLC/PRF/5 were determined quantitatively as 2.5-fold and 6.2-fold. The mRNA expressional difference of two major alleles was observed, which is consistent with that in the serum level. The culture cells carried heterozygous genotypes in rs4917 and rs4918, but homozygous one in rs2248690. It was unlikely that the imbalance was derived from the SNP located in the promotor site. Furthermore, to investigate the effect of mRNA degradation, RNA synthesis in the cell culture was inhibited potently by the addition of actinomycin-D. No marked change was apparent between the two alleles. The results indicated that the cis-regulatory expressional difference is expected to occur at the level of transcription or splicing of mRNA.

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“…To infer a biological relationship from pairwise genetic data in loci is based on population frequencies of the observed alleles shared by the pairs of individuals and on probability equations for genotype combinations 3,15 . The Likelihood ratio (LR) is calculated by using the frequency data to express the probability ratio of relatives to non-relatives.…”

Section: Introductionmentioning

confidence: 99%

“…The Likelihood ratio (LR) is calculated by using the frequency data to express the probability ratio of relatives to non-relatives. However, in some cases where the population frequencies of alleles may be unknown, or the ethnic origins may be unclear for foreign individuals, the LR based method fails to infer the relationship between two individuals 15 .…”

Section: Introductionmentioning

confidence: 99%

Development of a New Screening Method “Allele Matching Cut Off Score (AMCOS)” for Faster Kinship Analysis in Cases of Mass Disasters: A Proof of Concept Study

Kakkar

Sarma

Pannigrihi

et al. 2021

Preprint

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kinship analysis in forensic is based on calculation of respective kinship indices. But calculation of the same is possible only when the subject under identification has been associated to a particular population whose gene frequency data is available for the particular set of markers used in forensic practices. In case of the mass disasters where a huge number of individuals are to be identified, gathering the population frequency data and calculating the kinship indices can be an intricate progression requiring a lot of time and huge resources. The present study is based on allele matching score values which doesn’t require the use of allele frequency data to establish kinship. This method is based on the allele sharing approach which simply refers to the number of shared alleles (1 or 2) between two individuals; also known as identical by state (IBS) alleles which might have been inherited from a recent common ancestor in which the alleles are identical by descendent (IBD). In case of mass disasters this method can be used to narrow down the investigation by screening the number of related individuals which can further be confirmed with other tests if required. This method has been tested for various statistical parameters and has shown promising values which suggests the potential use of this method in forensic practice. This method has been tested on siblings and grandparent-grandchildren by using autosomal and X-STR markers both as the reference samples from parents can’t always be available. The present study also compares the results shown by autosomal and X-STR markers in siblings and grandparent-grandchildren identification, thereby suggesting the better set of markers for siblings and grandparent-grandchildren identification.

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Evaluation of the allele-sharing approach, known as the IBS method, in kinship analysis

Cited by 14 publications

References 22 publications

Evaluation of advanced multiplex short tandem repeat systems in pairwise kinship analysis

Evaluation of advanced multiplex short tandem repeat systems in pairwise kinship analysis

Allelic Imbalance of mRNA Associated withα2-HS Glycoprotein (Fetuin-A) Polymorphism

Development of a New Screening Method “Allele Matching Cut Off Score (AMCOS)” for Faster Kinship Analysis in Cases of Mass Disasters: A Proof of Concept Study

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