Evaluation of the alkylating activity of nitrosoureas by thin-layer densitometry

Potential of the NBP Method for the Study of Alkylation Mechanisms: NBP as a DNA-Model

“…Several applications have also involved the detection of organophosphorous pesticides, 144 estimation of insecticide levels on agricultural crop foliage, 145 and the validation of an oxidative treatment for alkylating agents present in residual waters. 146 NBP has very often been used as a TLC-developing agent, usually to detect organophosphorous pesticides 109,113,115 but also aza-heterocycles, such as diazinon, 147 trichothecene mycotoxins, 112 nitrosoureas, 148 or drugs such as thioTE-PA. 104,111 Addressing the detection of phosgene, a toxic atmospheric pollutant and an industrial chemical, Nakano et al developed a porous tape impregnated with a NBP solution able to detect 6 ppb of phosgene in air. 139 The formation of alkylating metabolites of chemicals and drugs has also been addressed: Guengerich et al used NBP to detect 2-cyanoethylene oxide, an alkylating metabolite of acrylonitrile; 149 Cioli et al tested the alkylating capacity of a putative metabolite of hycanthone, an antischistosomal drug; 102 and Padgette et al detected the formation of an epoxide metabolite of 1-phenyl-1-(aminomethyl)ethane hydrochloride.…”

“…NBP has very often been used as a TLC-developing agent, usually to detect organophosphorous pesticides ,, but also aza-heterocycles, such as diazinon, trichothecene mycotoxins, nitrosoureas, or drugs such as thioTEPA. , …”