Evaluation of the adsorption affinity of proteins to calcium hydroxyapatites by desorption and pre-adsorption methods

Adsorption of Bovine Serum Albumin and Lysozyme on Hydrophobic Calcium Hydroxyapatites

“…To the Ca(OH) 2 solution a waterethanol solution of DSOP and 2.4 ϫ 10 Ϫ2 mol of H 3 PO 4 was added immediately at room temperature. The details of the preparation and properties of OP-grafted CaHAP particles were reported in our previous paper (12). Some properties of the OP-grafted CaHAP particles are listed in Table 1.…”

“…These particles were produced in our previous study (12), and were synthesized from a coprecipitation reaction of Ca(OH) 2 and H 3 PO 4 with different amounts of disodium oleyl phosphate (DSOP) in water-ethanol mixtures. A solution containing 0.04 mol of Ca(OH) 2 was prepared in a 5.0-dm 3 screw-capped Nalgene polypropylene bottle by dissolving, with stirring, 3 g of Ca(OH) 2 in 2.0 dm 3 of deionized and distilled water free from CO 2 under an N 2 atmosphere.…”

“…If we can control the hydrophobicity of the CaHAP particle surface, corresponding to the regulation of the protein adsorption affinity of CaHAP, it will become an important technique for applying CaHAP to the purification, identification, fixation, and separation of proteins. Recently, we could control the hydrophobicity of the CaHAP particle surface by grafting various numbers of oleyl phosphate (OP) from 0 to 2.60 groups per nm 2 (n O ) onto the particle surface (12). This OP-grafted CaHAP particle seems to be adequate for understanding the role of the hydrophobic moiety of the CaHAP surface in the protein adsorption behavior.…”

Preparation and Characterization of Monosdispersed Metal Phosphate Particles and Characteristics of Protein Adsorption Onto Various Apatite Particles

“…19 and 20 38 . This result supports our findings described above that LSZ adsorbs weakly onto phosphate ions exposed on ac or bc faces of Hap while BSA adsorbs strongly onto positively charged sites on ac or bc faces of Hap.…”

“…In the past decade, the authors' group has been conducted fundamental studies on the adsorption of acidic bovine serum albumin (BSA), neutral myoglobin (MGB), and basic lysozyme (LSZ) onto various kinds of synthetic Hap particles. [34][35][36][37][38][39][40][41][42][43][44][45][46][47] In these studies, we investigated by changing many factors of Hap particles, such as calcium-to-phosphorus (Ca/P) atomic ratio, kinds of divalent cations (Ca, Sr and Ca-Sr solid solution), mean particle length, contents of carbonate ions incorporated and heat treatment temperature. The synthesis methods of various kinds of apatite particles as employed in the following sections were briefly described.…”