Evaluation of the Adherence ofCandidaSpecies to Urinary Catheters

Abstract

Catheter-associated urinary tract infections (CAUTIs) are the most common kind of nosocomial infection. Recent years have seen a significant increase in numbers of infections caused by yeasts of the genus Candida. The adherence of a microorganism to the host surface is a decisive factor in the success of colonization and the pathogenesis of infection. The objective of this work was to evaluate the adherence of species of the genus Candida to urinary catheters. In vitro adherence to the sections of latex and silicon catheters of Candida albicans and Candida parapsilosis were studied. Adherence was measured by counting the number of adhering viable cells and the results were expressed as Colonies Forming Units per mL. The results demonstrated that the latex catheter facilitated adherence more than the silicon catheter (p < 0.01). The adherence of the C. albicans was significantly greater than C. parapsilosis on latex, but it was similar on silicon.

Silicone colonization by non-Candida albicans Candida species in the presence of urine

“…C. glabrata, C. parapsilosis and C. tropicalis) were able to adhere to silicone (Fig. 1), which is in agreement with other studies examining Candida species adherence to silicone rubber (Busscher et al, 1997;Tamura et al, 2003). However, it is important to note that C. glabrata strains adhered to a higher extent than C. parapsilosis and C. tropicalis.…”

“…Nosocomial infections constitute a serious public health problem, as they are a major cause of morbidity and mortality, and cause an increased time of hospitalization with associated enhanced healthcare costs (Tamura et al, 2003). Urinary tract infection (UTI) is one of the most common types of nosocomial infections (Schaberg et al, 1991;Tamura et al, 2003) and more than 80 % of nosocomial UTIs are related to the use of medical devices such as urinary catheters (Febré et al, 1999;Harris et al, 1999;Douglas, 2002;Chow et al, 2008).…”

“…Urinary tract infection (UTI) is one of the most common types of nosocomial infections (Schaberg et al, 1991;Tamura et al, 2003) and more than 80 % of nosocomial UTIs are related to the use of medical devices such as urinary catheters (Febré et al, 1999;Harris et al, 1999;Douglas, 2002;Chow et al, 2008). Furthermore, Candida species are responsible for around 80 % of fungal infections in the hospital environment and 10-15 % of UTIs are caused by these micro-organisms (Amer et al, 2004).…”

Analysis of the in vitro adherence of Streptococcus mutans and Candida albicans

“…In addition, ATCC reference strains of C. albicans 10231 and S. mutans 25175 were also included. Based on the protocol of Tamura et al (34), isolates were cultivated at 37ºC for 18 h and their concentrations were standardized to 2.1 x 10 8 cells/mL using spectrophotometry. A total of 12 inocula were prepared using S. mutans (M), C. albicans (C), or both S. mutans and C. albicans (M+C).…”

“…In addition, the capacity of tissue invasion and adhesion to artificial materials such as probes, latex catheters, and several types of plastic material are responsible for causing important infections (34). In the case of dental biofilms, its role in coaggregation with S. mutans during the mechanisms of microbial adherence to the dental surface has been the focus of research (2,13,26,32,35).…”