Evaluation of the Accusport^®Lactate Analyser

Abstract

An investigation of the Accusport Lactate Analyser (ACC) was undertaken to assess its accuracy, linearity, reliability, and versatility. Accuracy was determined by comparing 32 blood assays on the ACC with those obtained using the Kodak Ektachem E250 (E250). Linearity was determined over the range 1.2-18.7 mmol/L. Reliability was measured at 1.7 (low) and 14.4 (high) mmol/L concentrations. Versatility involved determining the optimal sample volume, influence of haematocrit, and the effect of time delay from sampling to assay. Assays determined using the ACC confirm good agreement with the E250. The difference between the two methods compared for lactate determination does not exceed 1.1 mmol/L for concentrations up to approximately 20 mmol/L. Linearity is demonstrated by the regression equation y = 0.026 + 0.970x, r = 0.995. Reliability is expressed by the coefficient of variation of 7% (low) and 4.6% (high). The optimal sample size for accurate assay was determined to be between 20 microL and 50 microL. Mean lactate value did not vary significantly between a haematocrit of 35% and 45%. The Accusport is accurate, linear up to at least 18.7 mmol/L, has good reliability at high and low concentrations, and is able to analyse whole blood up to 15 minutes after sample application to the reagent strip.

Comparison of methods for the rapid laboratory assessment of children with malaria.

“…A number of other studies have examined the accuracy of the Accusport lactate analyzer in athletes, 14,15 neonates, 16,17 and critically ill patients. 18,19 Studies that have used BlandAltman analysis showed that mean differences (95% LOA) were similar to our findings, Ϫ0.2 (Ϫ1.2 to 0.8), 16 Ϫ0.3 (Ϫ1.33 to 0.69), 18 and for a study that tested 2 Accusport machines with machine A Ϫ0.4 (Ϫ1.8 to 1.1) and machine B Ϫ0.4 (Ϫ1.5 to 0.7).…”

Efeitos do exercício aeróbico e anaeróbico em variáveis de risco cardíaco em adultos com sobrepeso

“…After a 5-minute warm-up in the same cycle ergometer where the test would be conducted with a 15-Watt (W) load, the individuals pedaled at a constant speed of 28 Km.h -1 with an initial load of 25 W, which was increased by 25 W every three minutes. At the end of each 3-minute stage, the heart rate (HR) was measured with a Polar (S610 I) cardiac monitor and a 50-µL blood sample was collected from the ear lobe in heparinized capillary tubes; while the volunteer started the next exercise stage, the sample's lactate concentration was determined using an Accutrend portable lactate analyzer (Roche) 16 . The duration of this procedure was shorter than 30 seconds.…”

Análise da cinética de remoção de lactato em atletas de canoagem slalom

“…Foram coletados aproximadamente 25 µL de sangue por capilar do lóbulo da orelha com o uso de lancetas descartáveis marca Feathe. Para as análises foi utilizado lactímetro portátil (Accusport® -Boeringer Mannheim), anteriormente descrito e validado (FELL et al, 1998;INTERDONATO;LUETKEMEIER, 1999). A cinética de remoção do lactato foi realizada em quatro momentos distintos: anteriormente ao aquecimento (medida de repouso-valores Pré), logo após a saída dos competidores do rio (Pós 0'), 5 minutos depois (Pós 5') e 20 minutos após a coleta Pós 0' (Pós 20').…”