Evaluation of the Accuracy of Liquid-Based Oral Brush Cytology in Screening for Oral Squamous Cell Carcinoma

Deuerling, Lena; Gaida, Kristin; Neumann, Heinrich; Remmerbach, Torsten Wilhelm

doi:10.3390/cancers11111813

Cited by 22 publications

(28 citation statements)

References 36 publications

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“…Our data demonstrate that cells in the gingival sulcus may be a new entry point for the SARS-CoV-2 virus via a high expression of ACE2. In the present research, we first evaluated the expression of ACE2 in various sites of the oral cavity with noninvasive, convenient liquid-based cytology [9]. A recent study suggested that structural variations in human ACE2 may influence its binding with the SARS-CoV-2 spike protein [20].…”

Section: Discussionmentioning

confidence: 99%

“…Background characteristics included age, sex, smoking and alcohol consumption. The preparation procedure for liquid-based cytology differs from that of the conventional preparation of brush cytology [9]. In the conventional preparation, multiple exfoliated cells are spread out on multiple glass slides immediately after the collection of the cells.…”

Section: Methodsmentioning

confidence: 99%

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Oral Mucosa Could Be an Infectious Target of SARS-CoV-2

et al. 2021

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The World Health Organization reported that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission is caused by respiratory droplets and aerosols from the oral cavity of infected patients. The angiotensin-converting enzyme 2 (ACE2) is considered the host functional protein for SARS-CoV-2 infection. In this article, we first revealed that the positive proportion of ACE2 expression in gingival cells collected from the gingival sulcus was increased to the same level as the tongue. Our data demonstrate that cells in the gingival sulcus may be a new entry point for the SARS-CoV-2 virus via a high expression of ACE2. In addition, we first evaluated the expression of ACE2 in various sites of the oral cavity with noninvasive, convenient liquid-based cytology. The liquid-based cytology evaluation of oral tissue may provide a novel preventive medical avenue against COVID-19.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Oral Mucosa Could Be an Infectious Target of SARS-CoV-2

et al. 2021

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“…The use of cytobrush for further liquid-based cytology is a well-tolerated and minimally invasive technique for collecting samples [16,21]; however, a systematic review by Alsarraf et al indicates variable results without clear recommendations on the effectiveness of this technique for the diagnosis of lesions on the head and neck [22]. In this regard, our cytology results from samples performed before treatment, when compared with the biopsy (gold standard), showed a high sensitivity (over 80%) and specificity (100%).…”

Section: Discussionmentioning

confidence: 99%

Accuracy of liquid-based brush cytology and HPV detection for the diagnosis and management of patients with oropharyngeal and oral cancer

et al. 2021

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Objectives This study aims to evaluate the usefulness of liquid-based brush cytology for malignancy diagnosis and HPV detection in patients with suspected oropharyngeal and oral carcinomas, as well as for the diagnosis of tumoral persistence after treatment. Material and methods Seventy-five patients with suspicion of squamous cell carcinoma of the oropharynx or oral cavity were included. Two different study groups were analyzed according to the date of the sample collection: (1) during the first endoscopy exploration and (2) in the first control endoscopy after treatment for squamous cell carcinoma. Sensitivity, specificity, positive predictive value, negative predictive value, and accuracy for malignancy diagnosis as well as for HPV-DNA detection on brush cytologies were assessed. Results Before treatment, the brush cytology showed a sensitivity of 88%, specificity of 100%, and accuracy of 88%. After treatment, it showed a sensitivity of 71%, specificity of 77%, and accuracy of 75%. HPV-DNA detection in cytology samples showed a sensitivity of 85%, specificity of 100%, and accuracy of 91% before treatment and an accuracy of 100% after treatment. Conclusions Liquid-based brush cytology showed good accuracy for diagnosis of oropharyngeal and oral squamous cell carcinoma before treatment, but its value decreases after treatment. Nevertheless, it is useful for HPV-DNA detection, as well as to monitor the patients after treatment. Clinical relevance Brush cytology samples are reliable for the detection of HPV-DNA before and after treatment and may be a useful method to incorporate in the HPV testing guidelines.

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“…Liquid-based cytology (LBC) is a non-invasive technique that can be used to diagnose OED and OSCC. However, the false negative rate (FNR) has been reported to be 15.1-31.3% [7][8][9][10]; in particular, Kondo et al [10] showed that 81% cases of mild or moderate dysplasia were cytologically diagnosed as negative for any intraepithelial lesion or malignancy (NILM). False negative results can lead to undiagnosed carcinoma progression and mortality, if no further treatments are undertaken.…”

Section: Introductionmentioning

confidence: 99%

Novel Cytological Model for the Identification of Early Oral Cancer Diagnostic Markers: The Carcinoma Sequence Model

Kawaharada¹,

Yamazaki²,

Maruyama³

et al. 2021

Preprint

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Most oral squamous cell carcinomas (OSCCs) arise from oral epithelial dysplasia; however, there is no useful marker for early OSCC detection, likely owing to the inability to continuously observe the carcinoma sequence. We aimed to establish an experimental model to observe changes in the sequential expression pattern of mRNA and protein in the same rat using liquid-based cytology techniques. Cytology specimens were collected from a 4-nitroquinoline 1-oxide-induced rat tongue cancer model at 2, 5, 8, 11, 14, 17, and 21 weeks. We examined candidate biomarker expression using immunocytochemistry and quantitative real-time PCR. The percentage of positively stained nuclei was calculated as the labeling index (LI). All rats had OSCC of the tongue at 21 weeks. Brd4 (Brd4), Myc (c-Myc), and Tp53 (p53) mRNA levels were upregulated during progression from negative for intraepithelial lesion or malignancy to SCC. Brd4- and c-Myc-LI were increased in low- and high-grade squamous intraepithelial lesions and SCC specimens. p53-LI was significantly increased in SCC specimens. Our experimental model allowed the observation of sequential morphological changes and mRNA and protein expression patterns in the same rat during carcinogenesis. By reducing the false negative rate, BRD4 and c-Myc can be useful markers for the early detection of OSCC.

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Evaluation of the Accuracy of Liquid-Based Oral Brush Cytology in Screening for Oral Squamous Cell Carcinoma

Cited by 22 publications

References 36 publications

Oral Mucosa Could Be an Infectious Target of SARS-CoV-2

Oral Mucosa Could Be an Infectious Target of SARS-CoV-2

Accuracy of liquid-based brush cytology and HPV detection for the diagnosis and management of patients with oropharyngeal and oral cancer

Novel Cytological Model for the Identification of Early Oral Cancer Diagnostic Markers: The Carcinoma Sequence Model

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