Evaluation of the ability of natural and synthetic scaffolds in providing an appropriate environment for growth and chondrogenic differentiation of adipose-derived mesenchymal stem cells

Sheykhhasan, Mohsen; Qomi, Reza Tabatabaei; Kalhor, Neda; Mehdizadeh, Mohammad; Ghiasi, Mahdieh

doi:10.4103/0019-5413.164043

Cited by 19 publications

(11 citation statements)

References 62 publications

(85 reference statements)

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“…In addition, with an emphasis on adult stem cells rather than on embryonic stem cells, regenerative medicine programs are using ADSCs as more applicable adult stem cells to treat different diseases[ 4 ]. Today, Adipose-derived stromal cells are known as a rich source of MSCs which are considered a suitable case for repair and regeneration of various tissues because of their rapid proliferation and multilineage potential[ 2 - 5 ]. Several properties making scientists to pay attention to ADSCs include Immunomodulatory effects and secretion of a variety of growth factors and cytokine as well as anti-apoptosis and anti-inflammation potential[ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Adipose-derived stromal cell in regenerative medicine: A review

Qomi

Sheykhhasan

2017

WJSC

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The application of appropriate cell origin for utilizing in regenerative medicine is the major issue. Various kinds of stem cells have been used for the tissue engineering and regenerative medicine. Such as, several stromal cells have been employed as treat option for regenerative medicine. For example, human bone marrow-derived stromal cells and adipose-derived stromal cells (ADSCs) are used in cell-based therapy. Data relating to the stem cell therapy and processes associated with ADSC has developed remarkably in the past 10 years. As medical options, both the stromal vascular and ADSC suggests good opportunity as marvelous cell-based therapeutics. The some biological features are the main factors that impact the regenerative activity of ADSCs, including the modulation of the cellular immune system properties and secretion of bioactive proteins such as cytokines, chemokines and growth factors, as well as their intrinsic anti-ulcer and anti-inflammatory potential. A variety of diseases have been treated by ADSCs, and it is not surprising that there has been great interest in the possibility that ADSCs might be used as therapeutic strategy to improve a wider range of diseases. This is especially important when it is remembered that routine therapeutic methods are not completely effective in treat of diseases. Here, it was discuss about applications of ADSC to colitis, liver failure, diabetes mellitus, multiple sclerosis, orthopaedic disorders, hair loss, fertility problems, and salivary gland damage.

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Section: Introductionmentioning

confidence: 99%

Adipose-derived stromal cell in regenerative medicine: A review

Qomi

Sheykhhasan

2017

WJSC

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“…Sin embargo, mucho de los estudios encontrados en la literatura reportan estás técnicas para evaluar morfología celular pero no proporcionan un juicioso análisis e interpretación. (2,42) En cuarto lugar, en esta revisión sistemática los estudios de H. Park et. al (2010) A la luz de estos resultados se puede dilucidar que los ensayos metabólicos o combinación de ellos se posicionan como herramientas fundamentales para evaluar la proliferación y citotoxicidad celular.…”

Section: Discussionunclassified

“…Los matrices son estructuras de soporte fabricados artificialmente, los cuales pueden ser de origen natural o sintético (1,2). Dentro de los matrices naturales, los biopolímeros más usados son el colágeno, la gelatina, el quitosán y la fibrina (3).…”

Section: Introductionunclassified

Evaluation of the proliferation of adipose cells in fibrin and chitosan scaffolds: A systematic review

et al. 2020

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Introduction: Currently the matrices are used for the regeneration of organs and tissues. Among the most used natural polymers are chitosan and fibrin. These matrices provide a three-dimensional environment, with adequate biomechanical characteristics, which allow cell proliferation, migration and differentiation. The aim of the present study was to carry out a systematic review of those scientific works that try to “evaluate the in vitro proliferation capacity of adipose stem cells on fibrin and chitosan scaffolds”. Materials and Methods: A literature search was conducted in the PubMed, Proquest and Science direct databases, using specific search terms, from January 2006 to December 2019. Two investigators independently determined the eligibility of the studies. The articles were preselected considering the inclusion and exclusion criteria. Information on cell morphology, polymer dimensional change behavior, cell isolation and characterization, growth, viability, survival rate and evaluation / cytotoxicity of cells was extracted from the selected articles; when data were missing, the authors of the publication were contacted. The description of the methods to obtain cell proliferation data using metabolic tests such as: (MTT), (MTS), WST-1, LIVE-DEAD, scanning electron microscopy, transmission electron microscopy or fluorescence microscopy was considered. A checklist was used to assess the items and the concordance index was determined for the two researchers. Results: The initial search yielded 165 publications, of which 100 were excluded because they did not meet the inclusion criteria. Sixty-five were reviewed in full text, 58 were excluded because they found duplicates, presented a combination of chitosan and fibrin with other compounds, or because not all the information was found for the evaluation of methodological rigor. Finally, seven articles were selected and submitted to methodological evaluation. Conclusion: Selected studies demonstrate the biological and mechanical advantages of fibrin and chitosan biomaterials and their ability to stimulate cell proliferation. These biomaterials could be considered as options to be used as matrices in tissue engineering and regenerative medicine protocols. Future research on the evaluation of cell proliferation on any biomaterial should be aimed at proposing rigorous methodology that allows obtaining reliable results.

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“…Human adipose-derived stem cells (hADSCs) are a type of mesenchymal stem cells (MSCs) with chondrogenic differentiation ability [ 47 ]. Previous studies [ 48 , 49 ] have reported that the amplification of ADSCs on microcarriers followed by implantation has great benefits for cartilage regeneration.…”

Section: Overview Of the Advantages Of Microcarriers In The Treatmentmentioning

confidence: 99%

“…Compared to cartilage damage, adipose tissue damage is more easily accepted. ADSCs also present chondrogenic differentiation [ 47 ] and thus can be used as seed cells. Future applications of microcarriers in this direction will have clinical significance.…”

Section: Prospects For the Application Of Microcarriers In The Treatmmentioning

confidence: 99%