Evaluation of the 3M Petrifilm Enterobacteriaceae Count Plate Method for the Enumeration of Enterobacteriaceae in Foods

Silbernagel, Karen M; Lindberg, Kathryn

doi:10.4315/0362-028x-65.9.1452

Cited by 18 publications

(9 citation statements)

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“…A different result was reported by Silbernagel and Linderberg (2003), who in a study on artificial contamination with Proteus vulgaris at different inoculum levels in milk samples evaluated the performance of the Petrifilm EB system and compared the results to those obtained with the plate count method and the Most Probable Number (MPN) technique. In this study, the results showed that there was no statistical difference between the methods at an medium level of inoculation (10 3 CFU mL −1 ).…”

Section: Discussionmentioning

confidence: 89%

“…Silbernagel and Lindberg (2002) also conducted a similar study with 174 samples of naturally contaminated and 120 artificially contaminated dairy and non-dairy products, inoculated with three different inoculum levels (low, medium and high) and 45 pure non- Enterobacteriaceae strains and 65 pure cultures of Enterobacteriaceae , with the aim of evaluating the Petrifilm™ EB system and compare it against the performance of the VRBG Standard Method. The results showed that the Petrifilm™ EB system had an equal or better performance than the VRBG standard method and showed to be sensitive with a recovery rate of 97% of the Enterobacteriaceae present and also selective with only 16% of the non- Enterobacteriaceae being capable to grow on the Petrifilm™ EB plate.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the PetrifilmTM EB and TEMPO® EB systems with ISO 21528-2:2004 method for the count of Enterobacteriaceae in milk

Cirolini¹,

Miotto²,

Machado³

et al. 2013

Braz. J. Microbiol.

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The development of alternative microbiological techniques is driven by the necessity to meet the current needs to deliver rapid results in the manufacturing process of foods, but it is important that these methods be evaluated for each application. The objective of the present study was to assess the Petrifilm™ EB and the TEMPO® EB systems with ISO 21528-2:2004 for the count of Enterobacteriaceae in pasteurized and UHT milk samples. We analyzed the microflora of 141 pasteurized milk samples, 15 samples of artificially contaminated pasteurized milk and 15 samples of artificially contaminated UHT milk. Investigation of the method Petrifilm™ EB and ISO 21528:2 regression analysis showed a high correlation in the samples, r = 0.90 for the microflora of pasteurized milk, r = 0.98 for artificially contaminated pasteurized milk and r = 0.99 for the artificially contaminated UHT milk. In evaluating the system TEMPO EB ® method and ISO 21528:2 correlation was also significant in the analyzed samples, with r = 0.86 for the microflora of pasteurized milk, r = 0.96 for artificially contaminated pasteurized milk and r = 0.99 for artificially contaminated UHT milk. No statistically significant differences were observed between the three methods conducted to analyze artificially contaminated pasteurized and UHT milk at three inoculum levels. In conclusion, the Petrifilm™ EB system and the TEMPO® EB system may be an alternative to the ISO 21528-2:2004 for the Enterobacteriaceae assay for milk as because of the ease-of-operation and the time reduction achieved for conducting the microbiological assay using these systems.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Evaluation of the PetrifilmTM EB and TEMPO® EB systems with ISO 21528-2:2004 method for the count of Enterobacteriaceae in milk

Cirolini¹,

Miotto²,

Machado³

et al. 2013

Braz. J. Microbiol.

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“…Silbernagel and Lindberg (2002) worked with dairy and nondairy foods using naturally contaminated and artificially inoculated samples and concluded that the 3M Petrifilm EB plate method performed as well as or better than the standard VRBG method.…”

Section: Resultsmentioning

confidence: 97%

Evaluation of Enterobacteriaceae in the powdered milk production chain using both traditional (ISO 21528:2) and rapid (3M™ Petrifilm™) methods

2010

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This study sought to evaluate the presence of Enterobacteriaceae in several points of the powdered milk production chain (raw milk, pasteurized milk, powdered milk, and swabs of equipment and the hands of manipulators). There was a similarity between the ISO 21528:2 and the 3M Petrifilm Enterobacteriaceae Plate (EB) methods in all the samples tested. Enterobacteriaceae counts in raw milk were higher (p<0.001) than in other samples. Bacterial counts were similar for both sampling methods (p<0.01), except for raw milk. This observation highlights the fact that these samples were the main source of Enterobacteriaceae in the plant and reinforces the efficacy of the thermal treatment to reduce them. The mean Enterobacteriaceae counts were 5.68 and 5.83 log CFU/mL in raw milk and 0.45 and 0.97 log CFU/mL in powdered milk for the 3M Petrifilm EB plate and ISO 21528:2 methods, respectively. The industry used in this study can be considered a potential exporter of milk powdered to the European Union in terms of Enterobacteriaceae counts.

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“…As a conservative rule, values recorded as ,1.0 CFU/ml were transformed to 0.1 CFU/ml and those recorded as ,10.0 CFU/ml were transformed to 1.0 CFU/ml. Colony counts or MPN estimates were converted to log counts to more nearly match the underlying assumption of a normal distribution (13). Means, standard deviations, t test P values, and appropriate correlation coefficients were determined in R (11) using only the normalized values that were greater than 0.…”

Section: Methodsmentioning

confidence: 99%

Comparison of an Automated Most-Probable-Number Technique with Traditional Plating Methods for Estimating Populations of Total Aerobes, Coliforms, and Escherichia coli Associated with Freshly Processed Broiler Chickens

Line

Stern

Oakley

et al. 2011

Journal of Food Protection

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An instrument (TEMPO) has been developed to automate the most-probable-number (MPN) technique and reduce the effort required to estimate some bacterial populations. We compared the automated MPN technique with traditional microbiological plating methods and Petrifilm methods for estimating the total viable count of aerobic microorganisms (TVC), total coliforms (CC), and Escherichia coli populations (EC) on freshly processed broiler chicken carcasses (postchill whole carcass rinse [WCR] samples) and cumulative drip-line samples from a commercial broiler processing facility. Overall, 120 broiler carcasses, 36 prechill drip-line samples, and 40 postchill drip-line samples were collected over 5 days (representing five individual flocks) and analyzed by the automated MPN and direct agar plating and Petrifilm methods. The TVC correlation coefficient between the automated MPN and traditional methods was very high (0.972) for the prechill drip samples, which had mean log-transformed values of 3.09 and 3.02, respectively. The TVC correlation coefficient was lower (0.710) for the postchill WCR samples, which had lower mean log values of 1.53 and 1.31, respectively. Correlations between the methods for the prechill CC and EC samples were 0.812 and 0.880, respectively. The estimated number of total aerobes was generally greater than the total number of coliforms or E. coli recovered for all sample types (P < 2e⁻¹⁶). Significantly more bacteria were recovered from the prechill samples than from the postchill WCR or cumulative drip samples (P < 9.5e⁻¹² and P < 2e⁻¹⁶, respectively). When samples below the limit of detection were excluded, 92.1% of the total responses were within a single log difference between the traditional plating or Petrifilm methods and the automated MPN method.

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Evaluation of the 3M Petrifilm Enterobacteriaceae Count Plate Method for the Enumeration of Enterobacteriaceae in Foods

Cited by 18 publications

References 1 publication

Evaluation of the PetrifilmTM EB and TEMPO® EB systems with ISO 21528-2:2004 method for the count of Enterobacteriaceae in milk

Evaluation of the PetrifilmTM EB and TEMPO® EB systems with ISO 21528-2:2004 method for the count of Enterobacteriaceae in milk

Evaluation of Enterobacteriaceae in the powdered milk production chain using both traditional (ISO 21528:2) and rapid (3M™ Petrifilm™) methods

Comparison of an Automated Most-Probable-Number Technique with Traditional Plating Methods for Estimating Populations of Total Aerobes, Coliforms, and Escherichia coli Associated with Freshly Processed Broiler Chickens

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