Evaluation of the 3M™ Petrifilm™ Rapid E. coli/Coliform Count Plate for the Enumeration of E. coli and Coliforms: Collaborative Study, First Action: 2018.13

Abstract

Background The 3M™ Petrifilm™ Rapid E. coli/Coliform Count Plate is a selective and differential sample-ready-culture medium designed for the rapid enumeration of Escherichia coli (E. coli) and coliforms in the food and beverage industries. Objective The 3M Petrifilm Rapid E. coli/Coliform Count Plate was compared to the U.S. Food and Drug Administration Bacteriological Analytical Manual (BAM) Chapter 4 Enumeration of Escherichia coli and the Coliform Bacteria, the International Organization of Standards (ISO) 4832:2006 Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coliforms—Colony-count technique, and ISO 16649-2:2017 Microbiology of food and animal feeding stuffs—Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli—Part 2 Colony-count technique at 44 degrees C using bromo-4-chloro-3- indolyl beta-D-glucuronide methods for the enumeration of E. coli and coliforms in dry dog kibble. Method The candidate method was evaluated using two diluents, Butterfield's phosphate buffered diluent and peptone salt solution, in a paired study design with each reference method in a multi-laboratory collaborative study following the current AOAC Validation Guidelines. Three target contamination levels and an uninoculated control level were evaluated. Results The candidate and reference methods were not statistically different at each contamination level. Reproducibility values obtained during the collaborative study were similar between the candidate and reference methods. Conclusion These results demonstrate that the candidate method is equivalent to the reference methods. Highlight 3M Petrifilm Rapid E. coli/Coliform Count Plate was recommended for Official First Action status for enumeration of E. coli and coliforms in a broad range of foods and environmental surfaces.

What are the potential sources of on-farm contamination of raw milk butter with Escherichia coli?

“…Other coliforms can also be detected on Petrifilm, under the form of red colonies. Validation tests previously demonstrated that this method did not produce results significantly different from those obtained using method ISO 16649-2:2017 (Bird et al, 2020).…”

Drinking water quality assessment from water dispensers in an educational institution

“…Total coliform bacteria and fecal coliform bacteria were quantified by Most Probable Number (MPN) test. In addition, the microbiological analysis investigated for the presence of Escherichia coli (E. coli) and coliform bacteria using membrane filtration with 3M Petrifilm TM for E. coli/coliform count (EC) (Bird et al 2020). All analytical methods were performed according to the standard methods for the examination of water and wastewater (Public Health Act 1992; Eugene et al 2012).…”

Investigation of Citric Acid By-Products from Rice Produced by Microbial Fermentation on Growth Performance and Villi Histology of Thai Broiler Chicken (KKU 1)

“…The fecal content was pooled then examined at the Veterinary Diagnostic Laboratory Faculty of Veterinary Medicine Khon Kaen University, Thailand. A coliform/escherichia coli test was performed using the 3M Petrifilm Rapid E.coli/coliform counting plate, which was validated according to the AOAC Validation Guidelines following the AOAC Official Method of Analysis SM process [17]. A fecal sample was diluted with sterile saline peptone water in ratios ranging from 10:1 to 10:10, and the solutions were mixed thoroughly with a vortex mixer.…”