Evaluation of the 3M™ Molecular Detection Assay (MDA) 2 – Salmonella for the Detection of Salmonella spp. in Select Foods and Environmental Surfaces: Collaborative Study, First Action 2016.01

Abstract

The 3M™ Molecular Detection Assay (MDA) 2 - Salmonella uses real-time isothermal technology for the rapid and accurate detection of Salmonella spp. from enriched select food, feed, and food-process environmental samples. The 3M MDA 2 - Salmonella was evaluated in a multilaboratory collaborative study using an unpaired study design. The 3M MDA 2 - Salmonella was compared to the U.S. Food and Drug Administration Bacteriological Analytical Manual Chapter 5 reference method for the detection of Salmonella in creamy peanut butter, and to the U.S. Department of Agriculture, Food Safety and Inspection Service Microbiology Laboratory Guidebook Chapter 4.08 reference method "Isolation and Identification of Salmonella from Meat, Poultry, Pasteurized Egg and Catfish Products and Carcass and Environmental Samples" for the detection of Salmonella in raw ground beef (73% lean). Technicians from 16 laboratories located within the continental United States participated. Each matrix was evaluated at three levels of contamination: an uninoculated control level (0 CFU/test portion), a low inoculum level (0.2-2 CFU/test portion), and a high inoculum level (2-5 CFU/test portion). Statistical analysis was conducted according to the probability of detection (POD) statistical model. Results obtained for the low inoculum level test portions produced difference in collaborator POD values of 0.03 (95% confidence interval, -0.10 to 0.16) for raw ground beef and 0.06 (95% confidence interval, -0.06 to 0.18) for creamy peanut butter, indicating no statistically significant difference between the candidate and reference methods.

Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food

“…As LAMP is gaining popularity in clinical diagnostics and food testing, many commercially available LAMP-based systems and assays have been developed and some were validated for Salmonella detection in food (Yang et al, 2018). These include the 3M Molecular Detection Assay (MDA) Salmonella (3M Food Safety, St. Paul, MN, United States) in raw ground beef and wet dog food (Bird et al, 2013, 2014), the 3M MDA 2 – Salmonella in raw ground beef and creamy peanut butter (Bird et al, 2016), and the SAS Molecular Tests Salmonella detection kit (SA Scientific Ltd., San Antonio, TX, United States) in ground beef, beef trim, ground turkey, chicken carcass rinses, bagged mixed lettuce, and fresh spinach (Bapanpally et al, 2014). In the two studies evaluating the 3M MDA Salmonella in wet dog food against the FDA BAM method, an unpaired study design was used and fractional positive results were obtained with POD analysis showing the methods were comparable (Bird et al, 2013, 2014).…”

Loop-Mediated Isothermal Amplification forSalmonellaDetection in Food and Feed: Current Applications and Future Directions

“…Since 2008, the application of Salmonella LAMP assays in human food has expanded to numerous food matrices, such as chicken, turkey, pork, beef, produce, and milk. More recently, Salmonella LAMP assays have also been applied in animal food, that is, pet food, animal feed, and raw materials and ingredients (D'Agostino et al , 2015 ; Bird et al , 2016 ; Yang et al , 2016 ). Below we present some challenges commonly associated with foodborne pathogen detection and the promise that LAMP offers and some actual applications.…”

“…In this context, validations of several commercially available Salmonella LAMP detection kits have been completed, including 3M MDA Salmonella in raw ground beef and wet dog food (Bird et al , 2013 , 2014 ), 3M MDA 2— Salmonella in raw ground beef and creamy peanut butter (Bird et al , 2016 ), and SAS Molecular Tests Salmonella Detection Kit in ground beef, beef trim, ground turkey, chicken carcass rinses, bagged mixed lettuce, and fresh spinach (Bapanpally et al , 2014 ). Among them, 3M MDA 2— Salmonella has been approved for Official Method of Analysis (OMA) by AOAC International (OMA method No.…”

Prevalencia de Salmonella spp., en ganglios mesentéricos de porcinos en plantas de beneficio Colombianas

“…Moreover, more studies recommend molecular techniques, for this study the 3M™ Molecular Detection Assay 2-Salmonella was of great use, since no inhibitions nor false negative were present (data not shown) (32 Por último, la detección de Salmonella en ganglios linfáticos es pertinente, pues ésta muestra permite detectar si la fuente de origen es el cerdo y no una fuente ambiental, adicionalmente es indicada cuando se quiere determinar el origen de la cepa desde la granja (31). Por otra parte, cada vez son más los estudios que recomiendan el uso de técnicas moleculares, en este estudio resultó de gran utilidad el uso del sistema 3M™ Molecular Detection Assay 2-Salmonella; ya que no se presentaron inhibiciones ni resultados falsos negativos (datos no mostrados), (32).…”