Evaluation of temperature and guanidine hydrochloride-induced protein–liposome interactions by using immobilized liposome chromatography

Covalent conjugation of tetrameric bovine liver catalase to liposome membranes for stabilization of the enzyme tertiary and quaternary structures

“…On the other hand, conjugation of water-soluble proteins with electrically neutral membranes through linker molecules may allow maintaining their inherent structures and functions. Under the conditions causing conformational change of the membrane-linked proteins, hydrophobic interaction is induced between the proteins and the host membranes [10]. Wichmann et al [11] reported that anchoring the ␣-amylase and guanylase kinase to the surface of liposomes definitely alter the enzyme thermal stabilities.…”

Liposomes in chromatography

“…It was observed that the liposomeprotein binding depends considerably on the temperature of the interaction. Moreover it was suggested that the method is very useful for the investigation of the dynamic of protein-liposome interactions under stress conditions (Yoshimoto et al, 2006). Another ILC method was employed for the separation of compounds present in Danggui Buxue decoction interacting with liposome membrane.…”

Membranomics Research on Interactions between Liposome Membranes with Membrane Chip Analysis

“…It has been, furthermore, shown that the membranemembrane interactions, including membrane fusion, were caused by the hydrophobic interaction between membranes 20) , not only by the electrostatic interaction. These results were based on the study using the immobilized-liposome chromatography 8) . It is, therefore, considered that the interaction between free liposome and immobilized liposome could be also evaluated by using the above liposome-immobilized electrode.…”

“…In a series of reports, the immobilization technique of intact liposome on solid surfaces has been developed 8 ～ 16) to apply to the investigation of the protein-liposome interaction 1, 8, 15 ～ 19) , and the membrane-membrane interaction 16,20) . In the study in relation to the protein-liposome interaction, the systematic information for the surface property of proteins could be acquired 8) .…”