Evaluation of Telomerase Activity in Gingival Fibroblasts of Cyclosporine-Treated Patients

Biray, Cigir; Nizam, Nejat; Gündüz, Cumhur; Sönmez, Şule; Çavdar, C.; Sağlam, Funda; Atilla, Koray; Camsari, Taner

doi:10.1016/j.transproceed.2007.11.065

Cited by 1 publication

(2 citation statements)

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“…Previous studies suggested that underpinning this pathological overgrowth of gingival connective tissue were complex agonistic effects including elevated telomerase activity and inhibition of apoptosis in gingival fibroblast (Kantarci et al, 2007;Biray et al, 2008), which can be summarized as hyperactivity of resident cells. The hyperplastic gingival tissues used in current study were dissected from patients with gingival hyperplasia as a side effect following administration of immunosuppressive medication.…”

Section: Discussionmentioning

confidence: 99%

“…The hyperplastic gingival tissues used in current study were dissected from patients with gingival hyperplasia as a side effect following administration of immunosuppressive medication. Previous studies suggested that underpinning this pathological overgrowth of gingival connective tissue were complex agonistic effects including elevated telomerase activity and inhibition of apoptosis in gingival fibroblast (Kantarci et al, 2007;Biray et al, 2008), which can be summarized as hyperactivity of resident cells. Our observation of relatively lower MMP-1 expression in H-GMSC well coincided with previous report demonstrating the downregulation effects of immunosuppressive drugs on MMP-1 and MMP-1/TIMP-1 ratio in gingival fibroblasts (Sukkar et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of mesenchymal stem cells from human normal and hyperplastic gingiva

Tang

Xie

et al. 2010

Journal Cellular Physiology

150

154

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Human gingiva plays an important role in the maintenance of oral health and shows unique fetal-like scarless healing process after wounding. Here we isolate and characterize mesenchymal stem cells from human normal and hyperplastic gingival tissues (N-GMSC and H-GMSC, respectively). Immunocytochemical staining indicated that gingival lamina propria contained Stro-1 and SSEA-4 positive cells, implying existence of putative gingival MSC. Under attachment-based isolating and culturing condition, gingival MSC displayed highly clonogenic and long-term proliferative capability. By using single colony isolation and expansion approaches, we found both N-GMSC and H-GMSC possessed self-renewal and multipotent differentiation properties. N-GMSC and H-GMSC showed distinct immunoregulatory functions in a murine skin allograft setting via up-regulation of putative systemic regulatory T cells (Tregs). N-GMSC and H-GMSC were capable of regenerating collagenous tissue following in vivo transplantation, in which H-GMSC exhibited more robust regenerative capability. These findings suggest that gingival tissue contains tissue-specific mesenchymal stem cell population and is an ideal resource for immunoregulatory therapy due to its substantial availability and accessibility. In addition, gingival MSC over-activation may contribute to gingival hyperplastic phenotype.

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Section: Discussionmentioning

confidence: 99%