Evaluation of Taurine by HPTLC Reveals the Mask of Adulterated Edible Bird’s Nest

Abstract

Detection of amino acid is an effective and common method to determine adulteration in edible bird’s nest. Therefore, a simple and sensitive method was developed to detect taurine for determining adulteration in edible bird’s nest in the future. Sample was separated on precoated silica gel GF254 high-performance thin layer chromatographic plates. Separation of taurine was performed by n-propyl alcohol : ethanol : water : glacial acetic acid (5.2 : 0.8 : 2 : 2, v : v : v : v). Densitometric analysis of taurine was carried out in the absorbance mode at 485 nm. The method was validated for precision, intra- and interday variation, and recovery. This study proved that high-performance thin layer chromatography is a simple, rapid, precise, and selective method for qualitative and quantitative analysis of taurine in edible bird’s nest.

Prevalence of Nitrite and Nitrate Contents and Its Effect on Edible Bird Nest's Color

“…EBNs are reported to contain protein (62% to 63%), carbohydrate (25.62% to 27.26%), ash (2.1%), and lipid (0.14% to 1.28%) (Marcone ; Aswir and Wan Nazaimoon ). The amino acids found in EBNs are aspartic acid (Asp), threonine (Thr), serine (Ser), glutamic acid (Glu), proline (Pro), glycine (Gly), alanine (Ala), valine (Val), methionine (Met), isoleucine (Ile), leucine (Leu), tyrosine (Tyr), phenylalanine (Phe), histidine (His), lysine (Lys), tryptophan (Trp), and arginine (Arg) (Marcone ; Ma and Liu ; Teo and others ). Among them, aromatic amino acids (tyrosine, phenylalanine, tryptophan) possess phenyl rings that could react with nitric acid/nitrous acid (derived from HCl and sodium nitrate reaction or bacterial fermentation of bird soil) to form yellow EBNs through formation of aryl‐C‐N and NO 2 side group (Figure S1).…”

In-vivo hyperglycemic, antioxidant, histopathological changes, and simultaneous measurement of kaempferol verified by high-performance thin layer chromatography of Setaria italica in streptozotocin -induced diabetic rats

“…Thus, to identify herbal components, HPTLC fingerprinting is an accurate, linear & precise ( Cortés et al, 2014 ). In fact, this finger printing remains beneficial in herbal medicines or products quality control and also checking for the substances which adulterate the products ( Teo et al, 2013 ). Thus, to evaluate the different marketed pharmaceutical preparations, this HPTLC technique remains very helpful ( Gandhi et al, 2012 , Meena and Sandhya, 2013 , Patel et al, 2013 ).…”

Coriandrum sativum attenuates microglia mediated neuroinflammation and MPTP-induced behavioral and oxidative changes in Parkinson’s disease mouse model

“…Finger print analysis by various analytical techniques is a useful approach for the identification of major chemical compounds in various herbs and in quality control of herbal products (Teo et al, 2013[ 64 ]; Cortés et al, 2014[ 10 ]). Therefore, we performed HPTLC finger print analysis to assess the major phytonutrients present in CSE.…”