Evaluation of Taenia solium cyst fluid-based enzyme linked immunoelectro transfer blot for Neurocysticercosis diagnosis in urban and highly endemic rural population of North India

Recognition of immune reactive proteins as a potential multiepitope vaccine candidate of Taenia solium cysticerci through proteomic approach

“…Out of 23 proteins, 14 proteins had well‐defined functions while 9 proteins were uncharacterized. Our findings enlist presence of aldo keto reductase family proteins, which resonated our previous study on secretory proteins of T. solium 21 . This protein family is involved in different metabolic reactions, especially in oxidation–reduction 56 .…”

“…These proteins interact with host immune system and specific immunoglobulins are generated against them in patients' body fluids which makes them a suitable vaccine candidate. In our previous study, where we used CF to develop a very sensitive and specific EITB also encouraged us to explore CF vaccine potential where we identified several specific bands associated with NCC 21 . Further, we checked the antigenicity of these proteins and based on their antigenicity score, 8 of them were selected, having highest antigenicity for B‐cell epitope (TsM_000438100, TsM_000424500, TsM_000425500, TsM_000752100, TsM_000790700, TsM_000899700, TsM_000958600, and TsM_000676600) using BepiPred 2.0 server.…”

“…The CF was prepared as previously described 21,23 . In brief, T. solium viable cysts were isolated from infected pork muscle and washed vigorously thrice with cold phosphate buffer saline having 1% antibiotic (PenStrap; GIBCO) to remove the host tissues and debris.…”

“…The PVDF membrane was blocked with 5% skimmed milk in TBST (10 mM Tris‐HCl; pH 7.5; 15 mM NaCl; 0.05% tween 20) buffer for 2 h at RT and incubated with serum samples of NCC patient and healthy persons (1:100 dilutions) for overnight at 4°C. The pooled serum from symptomatic NCC patients ( n = 20) and healthy controls ( n = 20 each) were taken from our earlier study among urban NCC patients 21 . Subsequently, the PVDF membranes were washed thrice with TBST buffer for 15 min each followed by incubation with Rabbit‐anti‐human secondary IgG‐HRP conjugated antibody (Sigma‐Aldrich) for 1 h at RT.…”

“…The serum samples were used that have been collected during our previous study 21 . Institute's ethics committee and Biosafety committee of IIT Mandi approved the study after evaluating and following all the norms (IITM/IEC(H)/2023/AP/P4).…”