Evaluation of T7 and lambda phage display systems for survey of autoantibody profiles in cancer patients

Kalniņa, Zane; Siliņa, Karīna; Meistere, Irēna; Zayakin, Pawel; Rivosh, Alexander; Ābols, Artūrs; Leja, Mārcis; Minenkova, Olga; Schadendorf, Dirk; Linē, Aija

doi:10.1016/j.jim.2008.01.022

Cited by 44 publications

(42 citation statements)

References 29 publications

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“…351 Phage display is not only limited to filamentous phages, as libraries based on other phages such as lambda and T7 are also possible. 352, 353 …”

Section: Applications Of Virus-based Particlesmentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

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Virus-based nanomaterials are versatile materials that naturally self-assemble and have relevance for a broad range of applications including medicine, biotechnology, and energy. This review provides an overview of recent developments in “chemical virology.” Viruses, as materials, provide unique nanoscale scaffolds that have relevance in chemical biology and nanotechnology, with diverse areas of applications. Some fundamental advantages of viruses, compared to synthetically programmed materials, include the highly precise spatial arrangement of their subunits into a diverse array of shapes and sizes and many available avenues for easy and reproducible modification. Here, we will first survey the broad distribution of viruses and various methods for producing virus-based nanoparticles, as well as engineering principles used to impart new functionalities. We will then examine the broad range of applications and implications of virus-based materials, focusing on the medical, biotechnology, and energy sectors. We anticipate that this field will continue to evolve and grow, with exciting new possibilities stemming from advancements in the rational design of virus-based nanomaterials.

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“…351 Phage display is not only limited to filamentous phages, as libraries based on other phages such as lambda and T7 are also possible. 352, 353 …”

Section: Applications Of Virus-based Particlesmentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

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“…Combination of phage display with microarray technologies considerably improved the objective evaluation and throughput of the assays, allowing the testing of thousands of phages with only a few microliters of serum (6,8,15). This strategy, however, presents some limitations, such as the sequence of peptides that are displayed on the surface of the phage capsid (16), the presence of mimotopes (6) and the batch to batch reproducibility in microarray production, which is a common problem to other protein microarray formats.…”

Section: Colorectal Cancer (Crc)mentioning

confidence: 99%

Identification of MST1/STK4 and SULF1 Proteins as Autoantibody Targets for the Diagnosis of Colorectal Cancer by Using Phage Microarrays

Babel

Barderas

Dı́az-Uriarte

et al. 2011

Molecular & Cellular Proteomics

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The characterization of the humoral response in cancer patients is becoming a practical alternative to improve early detection. We prepared phage microarrays containing colorectal cancer cDNA libraries to identify phageexpressed peptides recognized by tumor-specific autoantibodies from patient sera. From a total of 1536 printed phages, 128 gave statistically significant values to discriminate cancer patients from control samples. From this, 43 peptide sequences were unique following DNA sequencing. Six phages containing homologous sequences to STK4/MST1, SULF1, NHSL1, SREBF2, GRN, and GTF2I were selected to build up a predictor panel. A previous study with high-density protein microarrays had identified STK4/MST1 as a candidate biomarker. An independent collection of 153 serum samples (50 colorectal cancer sera and 103 reference samples, including healthy donors and sera from other related pathologies) was used as a validation set to study prediction capability. A combination of four phages and two recombinant proteins, corresponding to MST1 and SULF1, achieved an area under the curve of 0.86 to correctly discriminate cancer from healthy sera. Inclusion of sera from other different neoplasias did not change significantly this value. For early stages (A؉B), the corrected area under the curve was 0.786. Moreover, we have demonstrated that MST1 and SULF1 proteins, homologous to phage-peptide sequences, can replace the original phages in the predictor panel, improving their diagnostic accuracy.

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“…The fraction of clones expressing peptides in frame in a naïve cDNA library may be as low as 6%. Additionally, phage with truncated constructs tends to outgrow clones with correctly displayed sequences [44, 45]. The quality can be improved by using ORF enriched DNA collections for library construction [43, 46].…”

Section: Proteomic Phage Displaymentioning

confidence: 99%

Interaction Analysis through Proteomic Phage Display

Sundell

Ivarsson

2014

BioMed Research International

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Phage display is a powerful technique for profiling specificities of peptide binding domains. The method is suited for the identification of high-affinity ligands with inhibitor potential when using highly diverse combinatorial peptide phage libraries. Such experiments further provide consensus motifs for genome-wide scanning of ligands of potential biological relevance. A complementary but considerably less explored approach is to display expression products of genomic DNA, cDNA, open reading frames (ORFs), or oligonucleotide libraries designed to encode defined regions of a target proteome on phage particles. One of the main applications of such proteomic libraries has been the elucidation of antibody epitopes. This review is focused on the use of proteomic phage display to uncover protein-protein interactions of potential relevance for cellular function. The method is particularly suited for the discovery of interactions between peptide binding domains and their targets. We discuss the largely unexplored potential of this method in the discovery of domain-motif interactions of potential biological relevance.

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Evaluation of T7 and lambda phage display systems for survey of autoantibody profiles in cancer patients

Cited by 44 publications

References 29 publications

Design of virus-based nanomaterials for medicine, biotechnology, and energy

Design of virus-based nanomaterials for medicine, biotechnology, and energy

Identification of MST1/STK4 and SULF1 Proteins as Autoantibody Targets for the Diagnosis of Colorectal Cancer by Using Phage Microarrays

Interaction Analysis through Proteomic Phage Display

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