Evaluation of sugarcane smut resistance in wild sugarcane (Saccharum spontaneum L.) accessions collected in Japan

Abstract

Sugarcane smut, caused by Sporisorium scitamineum, is one of the most important sugarcane diseases in Japan. Wild sugarcane, Saccharum spontaneum, is known to be a key breeding material to obtain high-yielding clones. In this study, we sought to identify Japanese wild sugarcane accessions with high resistance to smut. Thirty wild sugarcanes and three sugarcane cultivars were tested by the pinprick method. The results of the inoculation tests aided in identifying wild sugarcanes with high resistance to smut disease, namely JW90, Iriomote8, and Iriomote15. After screening the germplasm, progeny distribution of smut resistance from the inoculation test and dry matter productivity in the smut disease-free field were compared. The highly resistant wild sugarcane accession had a much better impact on progeny distribution of smut resistance compared with the susceptible accession. No relationship was found between smut resistance and dry matter productivity in both populations.

Arabidopsis-Based Dual-Layered Biological Network Analysis Elucidates Fully Modulated Pathways Related to Sugarcane Resistance on Biotrophic Pathogen Infection

“…Haplotype blocks of 1–100 Mbp in size, possibly representing introgressions, were recently shown as associated with relevant traits such as flowering time and dune adaptation of wild sunflowers ( Todesco et al, 2020 ). Although the ancestral S. spontaneum has been considered susceptible to smut ( Cheavegatti-Gianotto et al, 2011 ; Silva, 2017 ), wild accessions collected in Japan were recently found to be resistant ( Sakaigaichi et al, 2019 ).…”

Detection of a major QTL related to smut disease resistance inherited from a Japanese wild sugarcane using GRAS-Di technology

“…Like most agronomic traits of sugarcane, smut disease resistance is thought to be a quantitative characteristic that is difficult to investigate genetically (Schaker et al 2016). Our group identified highly smut disease resistant accessions of S. spontaneum collected in Japan, including 'Iriomote8' (Sakaigaichi et al 2018). Using the mapping population derived from a resistant variety 'Yaenoushie', in which resistance is inherited from 'Iriomote8', we detected a major QTL related to smut disease resistance with a markedly high LOD score (Fig.…”

“…KARC/NARO conserves approximately 200 accessions of Japanese Saccharum spontaneum collected by NARO Genebank project. Our group identified highly smut disease resistant accessions within this collection (Sakaigaichi et al 2018) and developed mapping populations using the identified resistant accessions. Toyota Motor Corporation invented Genotyping Random Amplicon Sequence-Direct (GRAS-Di) technology, which enables rapid and reproducible genotyping of sugarcane (Enoki 2019, Enoki andTakeuchi 2018).…”