Evaluation of subculture ages on organogenic response from root callus and SPAR based genetic fidelity assessment in the regenerants of Hibiscus sabdariffa L.

Konar, Saptarshi; Adhikari, Sinchan; Karmakar, Joydeep; Ray, Anirban; Bandyopadhyay, Tapas Kumar

doi:10.1016/j.indcrop.2019.04.018

Cited by 17 publications

(8 citation statements)

References 57 publications

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“…This suggests that long-term subculture callus in peach trees has a significant change in epigenetic modifications, which coincides with a previous report that numerous of epigenetic modifications occur during the process of callus-based regeneration in plant [84]. Change in epigenetic landscape is known to be accompanied by transition of cell fate [85,86]. Here, our results indicate that aged callus cells with long-term in vitro culture lost root regeneration ability.…”

Section: Epigenetic Regulation In Callus Adventitious Rooting In Peachsupporting

confidence: 92%

Callus Induction and Adventitious Root Regeneration of Cotyledon Explants in Peach Trees

Gao,

Liu,

Liao

et al. 2023

Horticulturae

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Callus induction is a key step in establishing plant regeneration and genetic transformation. In this study, we present a comprehensive large-scale investigation of the callus induction rate (CIR) in peach trees, which revealed significant variability within the peach germplasm. Notably, the late-maturing cultivars exhibited significantly higher levels of CIR. Moreover, cultivars characterized as having high CIR exhibited potential for the development of adventitious roots (ARs) during callus induction, and a positive correlation was observed between CIR and the ability to regenerate ARs. However, long-term subculture callus lost root regeneration capacity due to changes in cellular morphology and starch and flavonoid content. Additionally, PpLBD1 was identified as a good candidate gene involved in the regulation of callus adventitious rooting in peach trees. Our results provide an insight into the mechanisms underlying callus induction and adventitious root development and will be helpful for developing regeneration systems in peach trees.

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Section: Epigenetic Regulation In Callus Adventitious Rooting In Peachsupporting

confidence: 92%

Callus Induction and Adventitious Root Regeneration of Cotyledon Explants in Peach Trees

Gao,

Liu,

Liao

et al. 2023

Horticulturae

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“…This factor becomes crucial to assess the genetical stability of in vitro-cultured plants, making their valorization easier. Moreover, long-term subcultures constitute an efficient strategy to improve interesting phenomena under in vitro conditions, such as rooting (Mendonça et al, 2019;Wang and Yao, 2020), plant regeneration (Konar et al, 2019) and callus induction (Nakasha et al, 2016), very useful for biotechnological production of by-products from medicinal plants.…”

Section: Discussionmentioning

confidence: 99%

Machine Learning Unmasked Nutritional Imbalances on the Medicinal Plant Bryophyllum sp. Cultured in vitro

et al. 2020

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Plant nutrition is a crucial factor that is usually underestimated when designing plant in vitro culture protocols of unexploited plants. As a complex multifactorial process, the study of nutritional imbalances requires the use of time-consuming experimental designs and appropriate statistical and multiple regression analysis for the determination of critical parameters, whose results may be difficult to interpret when the number of variables is large. The use of machine learning (ML) supposes a cutting-edge approach to investigate multifactorial processes, with the aim of detecting non-linear relationships and critical factors affecting a determined response and their concealed interactions. Thus, in this work we applied artificial neural networks coupled to fuzzy logic, known as neurofuzzy logic, to determine the critical factors affecting the mineral nutrition of medicinal plants belonging to Bryophyllum subgenus cultured in vitro. The application of neurofuzzy logic algorithms facilitate the interpretation of the results, as the technology is able to generate useful and understandable “IF-THEN” rules, that provide information about the factor(s) involved in a certain response. In this sense, ammonium, sulfate, molybdenum, copper and sodium were the most important nutrients that explain the variation in the in vitro culture establishment of the medicinal plants in a species-dependent manner. Thus, our results indicate that Bryophyllum spp. display a fine-tuning regulation of mineral nutrition, that was reported for the first time under in vitro conditions. Overall, neurofuzzy model was able to predict and identify masked interactions among such factors, providing a source of knowledge (helpful information) from the experimental data (non-informative per se), in order to make the exploitation and valorization of medicinal plants with high phytochemical potential easier.

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“…The results obtained that genetically the subcultured explants relatively stable, some species show subculture frequencies ranging from 3 to 9 times still showing good growth. Konar et al [21] showed the highest shoot multiplication in Hibiscus sabdariffa was obtained in subculture 8. Harianja et al [22] also stated that subculture in Medicago sativa can still be carried out until subculture 3 because it remains genetically stable.…”

Section: Resultsmentioning

confidence: 98%

Effect of gamma iradiated seeds of Jabon Merah (Neolamarckia macrophylla (Wall.) Bosser) to genetic diversity

Larekeng

Rachmat

Iswanto

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Jabon merah (Neolamarckia macrophylla (Wall.) as one of the potential industrial forest plantations to meet the need for wood that has a moderate level of genetic diversity. This study aims to analyze the morphological and genetic characters and analyze the stability of genes in explants from red jabon seeds induced by gamma rays at doses of 0 gray, 5 gray, 10 gray, 15 gray, 30 gray, 45 gray and 60 gray, 75 gray, 90 gray, 120 gray and 240 gray. The induced seeds were cultured in vitro. The subculture was started with an initialization on MS0 medium and repeatedly cultured. Finally, the LD50 value was determined using curve fit analysis, explant morphological analysis with SPSS, genetic analysis of explants using RAPD markers and SSR/Microsatellite. The DNA analysis with 9 SSR/Microsatellite and RAPD marker primers showed that most of the induction doses produced more fragments than the control.

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Evaluation of subculture ages on organogenic response from root callus and SPAR based genetic fidelity assessment in the regenerants of Hibiscus sabdariffa L.

Cited by 17 publications

References 57 publications

Callus Induction and Adventitious Root Regeneration of Cotyledon Explants in Peach Trees

Callus Induction and Adventitious Root Regeneration of Cotyledon Explants in Peach Trees

Machine Learning Unmasked Nutritional Imbalances on the Medicinal Plant Bryophyllum sp. Cultured in vitro

Effect of gamma iradiated seeds of Jabon Merah (Neolamarckia macrophylla (Wall.) Bosser) to genetic diversity

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