Evaluation of structure and bioprotective activity of key high molecular weight acylated anthocyanin compounds isolated from the purple sweet potato (Ipomoea batatas L. cultivar Eshu No.8)

Anthocyanin extracts of lingonberry (Vaccinium vitis‐idaea L.) attenuate serum lipids and cholesterol metabolism in HCD‐induced hypercholesterolaemic male mice

“…The eluate was monitored at 520 and 280 nm respectively. External standard method was used for quantification of anthocyanins of LAE according to previously published reports (Zhang et al ., ; Xu et al ., ; Luo et al ., ). The standard sample (cyanidin‐3‐O‐glucoside, C3G) was dissolved with ultrapure water with a stock solution of 1 mg mL −1 , and then diluted into six levels in succession.…”

Effect of ultrasonic and ball‐milling treatment on cell wall, nutrients, and antioxidant capacity of rose (Rosa rugosa) bee pollen, and identification of bioactive components

“…The DPPH-scavenging activity was determined using the method described in our previous work, with some modifications. 19 First, 200 μL of DPPH (10 mmol L −1 ) and 40 μL of sample were added to microplate. The mixture was placed in the dark for 30 min, followed by measurement of the absorbance at 517 nm.…”

“…The ABTS radical scavenging capacity assay was carried out in accordance with previous reports with some modifications. 19,20 Briefly, the ABTS ·+ solution was prepared as follows: the mixture of 7 mmol L −1 ABTS ·+ solution and phosphate buffered saline (pH 7.4) were reacted in the dark for 16 h. Then the ABTS ·+ solution was diluted with phosphate buffered saline to an absorbance at 0.70 ± 0.02 at 734 nm. Next, the microplate containing 200 μL of ABTS ·+ solution and 10 μL of sample was placed in the dark for 6 min.…”

“…The ethanol extract of rose bee pollen was identified according to the method reported previously, with some modifications. 19,21 The sample was filtered through a 220 nm membrane filter, followed by the analysis using UPLC-ESI-QTOF-MS/MS (Waters Corp.). The separation of compounds was conducted by a Hypersil…”

Isolation and identification of two major acylated anthocyanins from purple sweet potato (Ipomoea batatas L. cultivar Eshu No. 8) by UPLC‐QTOF‐MS/MS and NMR

“…8) have been identified as cyanidin or peonidin 3‐sophoroside‐5‐glucoside based and acylated with one or two phenolic acid, such as caffeic acid, p ‐hydroxybenzoic acid and ferulic acid on the 3‐sophorosy moiety (Terahara et al ., ). According to the previous report of our laboratory (Zhang et al ., , ; Wang et al ., ; Luo et al ., ), peonidin 3‐caffeoyl‐ p ‐hydroxybenzoyl sophoroside‐5‐glucoside and peonidin 3‐caffeoyl‐feruloyl sophoroside‐5‐glucoside are two major anthocyanins from purple sweet potato. The anthocyanins in purple sweet potato primarily occur as acylated compounds, and their content accounts for more than 93% (Truong et al ., ).…”