Evaluation of structural changes and intracellular substance leakage of
            <i>Escherichia coli</i>
            O157:H7 induced by ohmic heating

Tian, Xiaojing; Shao, Longtan; Yu, Qunli; Liu, Y.; Li, X.; Dai, Ruitong

doi:10.1111/jam.14411

Cited by 15 publications

(4 citation statements)

References 32 publications

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“…Meanwhile, the protein concentration of the supernatant was measured by the BCA method. Absorbance values were determined at 450 nm using a microplate reader (Unico (Molecular Devices SpectraMax250, United States) ( Tian et al, 2019 ; Liao et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

The Changes of KCNQ5 Expression and Potassium Microenvironment in the Retina of Myopic Guinea Pigs

et al. 2021

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KCNQ5 is suggestively associated with myopia, but its specific role in the myopic process has not been studied further. The aim of this study was to investigate the expression of potassium channel gene KCNQ5 and the changes of K+ microenvironment within the retina of form deprivation myopia (FDM) guinea pigs. A total of 60 guinea pigs were randomly divided into the normal control (NC) group, the self-control (SC) group, and the form-deprivation (FD) group for different treatments. Molecular assays and immunohistochemistry (IHC) were conducted to measure the expression and distribution of KCNQ5-related gene and protein in the retina. We determined the K+ concentration in the retina. In addition, the possible effects of form deprivation on potassium ionic currents and the pharmacological sensitivity of KCNQ5 activator Retigabine and inhibitor XE991 to the M-current in RPE cells were investigated using the patch-clamp technique. As a result, FD eyes exhibited more myopic refraction and longer AL. The mRNA and protein levels of KCNQ5 significantly decreased in the FD eyes, but the K+ concentration increased. In addition, the M-type K+ current [IK(M)] density decreased in FD RPE cells, and were activated or inhibited in a concentration-dependent manner due to the addition of Retigabine or XE991. Overall, KCNQ5 was significantly downregulated in the retina of FD guinea pigs, which may be associated with the increasing K+ concentration, decreasing IK(M) density, and elongating ocular axis. It suggested that KCNQ5 may play a role in the process of myopia, and the intervention of potassium channels may contribute to the prevention and control of myopia.

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Section: Methodsmentioning

confidence: 99%

The Changes of KCNQ5 Expression and Potassium Microenvironment in the Retina of Myopic Guinea Pigs

et al. 2021

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“…Where CFU/mL CL was the counts on Compass Listeria Agar; CFU/ mL CLTSA was the counts on Compass Listeria Agar +TSA (22).…”

Section: Enumeration Of Viable and Injured Cellsmentioning

confidence: 99%

“…Many researchers have recently researched the use of ohmic heating to inactivate foodborne bacteria (13,18,22). Previous studies showed that OH is affected by extrinsic factors such as voltage and frequency and intrinsic factors such as features of lipids, proteins, carbohydrates and their quantities in food.…”

Section: Introductionmentioning

confidence: 99%

Determination of the effect of milk fat on the inactivation of Listeria monocytogenes by ohmic heating

ÖZKALE¹,

KAHRAMAN²

2023

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Research in recent years has focused on innovative technologies that provide pathogen inactivation without damaging the structural properties of foods. Ohmic heating (OH) is an innovative technology, that provides an effective microbial inactivation with massive and rapid heating. This study aims to determine the effects of milk fat on the inactivation of Listeria monocytogenes by OH with a low voltage gradient.L. monocytogenes (ATCC 13932) inoculated 3.1%, 1.5%, and 0.1% fat-milk samples were heated up to 62°C by OH and conventional heating (CH) process. OH treatment lead to the inactivation of L. monocytogenes in both 1.5% and 0.1% groups and led to approximately 5.30 log decrease, however, there was a 3.10 log decrease in the 3.1% group at 6 min. CH lead to a few reduction as 0.21, 0.29 and 0.39 log in 3.1%, 1.5% and 0.1% fat-milk respectively. In OH, the sublethal injury ratio was higher than CH in all milk groups. However, OH did not statistically change color and pH values at the 6 th min of the process, had a significant effect on hydroxymethylfurfural value only in 3.1% fatmilk. In conclusion, the increased fat content may have important inhibitory effects on pathogen inactivation in OH. Thus, the OH conditions should be chosen carefully to sufficient inactivation of pathogens in milk with high-fat content.

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“…The practicality and cost-effectiveness of the OH system compared to other thermal heating methods have made it a promising application in the processing of dairy products (Bosi et al 2013). Although there are many studies on the application of OH in different food matrices (Park and Kang 2013, Cho et al 2017, Tian et al 2019, Pires et al 2020, Özkale and Kahraman 2023, research on pathogen inactivation in proteinenriched milks is quite limited as far as we know. This study aimed to investigate the effects of the OH process applied with different voltage gradients on L. monocytogenes inactivation and some quality parameters in protein-enriched milks.…”

Section: Introductionmentioning

confidence: 99%

Ohmic heating application with different electric fields on inactivation of Listeria monocytogenes in protein-enriched cow milk

Ayyıldız,

Kahraman

2024

Polish Journal of Veterinary Sciences

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The aim of this study was to determine the effects of an ohmic heating (OH) process with different electric field intensities on Listeria monocytogenes inactivation in protein-enriched cow milk. Protein powder was added at rates of 2.5%, 5% and 7.5% in 1.5% fat content milk, and L. monocytogenes (ATCC 13932) strain was then inoculated into the samples. The OH process was carried out in a laboratory-type pilot unit created using stainless steel electrodes, a K-type thermocouple, a datalogger and power supply providing AC current at 0-250 V, 10 A. The inoculated milk samples were heated to 63°C by applying an electric field intensity of 10V/cm and 20V/cm. L. monocytogenes counts, pH, color measurement and hydroxymethylfurfurol levels were then determined. OH applied with an electric field intensity of 10 V/cm caused an average decrease of 5 logs in L. monocytogenes level in the samples containing 2.5% protein and decreased below the detection limit (<1 log) at the 9th minute (p<0.05). Similarly, application of an electric field intensity of 20 V/cm in milk containing 2.5% and 5% protein caused the L.monocytogenes level to decrease below the detection limit (<1 log) at 2 minutes 30 seconds (p<0.05). No change was observed in the L* (brightness) values of the samples but it was determined that there was a slight increase in pH, a* (redness) and b* (yellowness) values compared to the control group. It was observed that the inactivation of L. monocytogenes by OH depends on the duration of the OH process, protein concentration in the milk and the applied voltage gradient.

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Evaluation of structural changes and intracellular substance leakage of Escherichia coli O157:H7 induced by ohmic heating

Cited by 15 publications

References 32 publications

The Changes of KCNQ5 Expression and Potassium Microenvironment in the Retina of Myopic Guinea Pigs

The Changes of KCNQ5 Expression and Potassium Microenvironment in the Retina of Myopic Guinea Pigs

Determination of the effect of milk fat on the inactivation of Listeria monocytogenes by ohmic heating

Ohmic heating application with different electric fields on inactivation of Listeria monocytogenes in protein-enriched cow milk

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