Evaluation of stress in laboratory rabbits used for teaching purposes

Urbanová, Marie; Kramárová, E; Chloupek, Jan; Najmanová, Martina

doi:10.2754/avb201988020249

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…A study model of the uterus duplex in the rabbit is, in our opinion, suitable for examining other preparations. Besides macroscopic observation, the monitoring of biochemical and haematological indices, and their subsequent inclusion in the results is also advisable (Pecin et al 2017;Urbanová et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the effect of two anti-adhesion materials on the formation of postoperative adhesions after myomectomy – an experimental study on rabbit does

et al. 2021

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This study compares the effect of Hyalobarrier Gel and 4DryField® PH on the formation of postoperative adhesions after myomectomy in an experimental study on rabbit does. Thirty experimental animals were divided into three groups: group A – control, group B – use of 4DryField® PH, and group C – use of the Hyalobarrier Gel. A simulated myomectomy was performed in each group. The animals were euthanised 14 days after the procedure and the antiadhesive effect of the above-mentioned preparations was evaluated according to the criteria. Statistical evaluation clearly showed differences in the antiadhesive effect in the scoring systems for groups B and C compared to control group A at a high level of significance. We demonstrated an obvious, statistically confirmed antiadhesive effect of the Hyalobarrier Gel and 4DryField® PH in an experimental model of the rabbit uterus. Comparing both preparations, the Hyalobarrier Gel shows a higher level of effectiveness. Based on the results of the experiment, both preparations can be unambiguously recommended for routine practice.

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Section: Discussionmentioning

confidence: 99%

Comparison of the effect of two anti-adhesion materials on the formation of postoperative adhesions after myomectomy – an experimental study on rabbit does

et al. 2021

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“…Stress has commonly been measured not only by cortisol levels in blood but also as cortisol metabolites in feces, hair, and urine [1]. Neutrophil-lymphocyte ratio has been employed as a stress measurement as well [2], because the duration of stress affects leucocyte populations [3]. However, a major issue for animal welfare is that these techniques involve invasive procedures which may themselves cause a stress response and therefore affect the measurement of interest [4].…”

Section: Introductionmentioning

confidence: 99%

Preliminary Study of Body Temperature Emissivity in Rabbits Selected for Litter Size Residual Variability

2021

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A divergent selection for litter size residual variability has been carried out in rabbits during 12 generations. Litter size residual variability was estimated as phenotypic variance of litter size within females after correcting for the year-season and the parity-lactation status effects. Stress causes an increase in core body temperature. Infrared thermography (IRT) has been shown to be a useful technique for identifying changes in body temperature emissivity. The aim of this work is to study the correlated response to selection for litter size residual variability in body temperature emissivity at natural mating. Natural mating can be considered a stressful stimulus for does. Temperature was measured in the eyeball by IRT before mating (basal temperature) and after 5 min, 30 min, and 60 min in does of the lines selected to decrease and to increase litter size residual variability (i.e., the Low and the High lines). Both lines showed similar basal temperature. Eyeball temperature was increased slightly in the Low line from basal state to 5 min after stressful stimulus (from 35.69 °C to 36.32 °C), and this increase remained up to 60 min after stress (36.55 °C). The High line showed a higher temperature than the Low line at 30 min (+0.96 °C, p = 0.99). At 60 min, temperature was similar between lines. The evolution of temperature was different between lines: the High line reached the peak of temperature later than the Low line (at 30 min vs. 5 min), and its peak was higher compared to the Low line (36.95 °C vs. 36.32 °C). In conclusion, the does selected for reducing litter size variability showed a lower increase in temperature after a stressful stimulus, therefore showing lower stress and consequently better welfare.

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“…In pigs, no study of this type has been published yet. Only one similar study has been carried out so far by Urbanova et al (2019), focusing on stress in rabbits which showed that animals can get used to repeated manipulation; with each subsequent manipulation there was a lower hormonal stress response.…”

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confidence: 99%

Sampling methods for determination of cortisol in pig saliva and their use in the assessment of pig welfare

2022

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This study focused on finding new information regarding the assessment of pig saliva cortisol samples in terms of practical effects of the sampling, sample storage conditions, and their laboratory analysis. The study was divided into two experiments. The first experiment was focused on finding the effect of sampling time on cortisol concentrations in pig saliva. The second experiment was focused on determining the effect of storage conditions on the value of salivary cortisol. Before the initiation of the study, we tested which one of the commercially available ELISA kits would be the most suitable for our experiments. Simultaneously, we carried out a pre-study to evaluate the effect of relocation and change in the housing type on the concentration of salivary cortisol in gestating sows. The samples were obtained by oral cavity swabbing, using a standard cotton swab. In the first study, piglets were examined at the age of 4 ± 1 days, and breeding management routine procedures were used as a stress factor. In the second study, the piglets were examined immediately after weaning (at 28 ± 2 days of age). The Cortisol EIA kit was found to be statistically more accurate and thus a more suitable ELISA kit for our experiment. Analysis of the relocation effect and the effect of change in the housing type showed that relocation does not seem to be a stress factor for gestating sows as no significant changes were observed in salivary cortisol concentration (P > 0.5); however, the change in the housing type lead to a significant increase in salivary cortisol (P < 0.001). In the first study, we determined using the ELISA method that the most significant difference occurred in 40 min (P < 0.01), which suggests that the best time for a sampling in order to assess salivary cortisol concentration is 40 min after stress induction by routine procedures. The conclusion of the second study was that in the monitored period of 60 h (P < 0.05), cortisol concentration decreased depending on the storage temperature. The decrease started between 48 and 60 h which showed that cortisol is stable in the saliva sample for at least 48 h. These findings will be further applied in our following studies focused on assessment of salivary cortisol concentration after stress induction.

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Evaluation of stress in laboratory rabbits used for teaching purposes

Cited by 3 publications

References 17 publications

Comparison of the effect of two anti-adhesion materials on the formation of postoperative adhesions after myomectomy – an experimental study on rabbit does

Comparison of the effect of two anti-adhesion materials on the formation of postoperative adhesions after myomectomy – an experimental study on rabbit does

Preliminary Study of Body Temperature Emissivity in Rabbits Selected for Litter Size Residual Variability

Sampling methods for determination of cortisol in pig saliva and their use in the assessment of pig welfare

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