Evaluation of standardized performance test methods for biomedical Raman spectroscopy

Fales, Andrew M.; Ilev, Ilko K.; Pfefer, T. Joshua

doi:10.1117/1.jbo.27.7.074705

Cited by 9 publications

(7 citation statements)

References 26 publications

(32 reference statements)

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“…Nevertheless, in general, chemometric models implemented in the industry for data analysis are often application-specific and lack transferability. The academia has made good efforts to understand, 96 with basic research and interlaboratory studies, and remove, 97,23 though RMs as well as chemometrics or protocols development, the setup-induced spectral variations, but manufacturers and standardization bodies engagement is key. Raman equipment and firmware offer different options for calibration and validation depending on the manufacturer/model/technique and on the target user, but they should provide complete calibration modules as well as open access to the real raw data and technical details of the applied corrections.…”

Section: Discussionmentioning

confidence: 99%

Review of Existing Standards, Guides, and Practices for Raman Spectroscopy

Ntziouni

Thomson²,

Xiarchos

et al. 2022

Appl Spectrosc

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Over the past decades Raman spectroscopy has been extensively used both on an industrial and academic level. This has resulted in the development of numerous specialised Raman techniques and Raman active products, which in turn has led to the adoption and development of standards and norms pertaining to Raman unit’s calibration, performance validation and interoperability. Purpose of the present review is to list, classify and engage in a comprehensive analysis of the different standards, guides and practices relating to Raman spectroscopy. Primary aim of the review is to consider the commonalities and conflicts between these standards and norms and to identify any missing aspects. Standardisation in the field of Raman spectroscopy is dominated by the work of American institutions, namely the American Society of Testing Materials (ASTM or ASTM International), with several active standards in place pertaining to terminology, calibration, multivariate analysis and specific applications, and the National Institute of Standards and Technology (NIST), providing numerous standard reference materials. The industrial application of Raman spectroscopy is dominated by the pharmaceutical industry. As such, pharmacopoeias provide not only important information in relation to pharmaceutical-related applications of Raman spectroscopy, but also invaluable insight, usually by referring to ASTM and NIST standards, into the basic principles of Raman spectroscopy and important aspects that include calibration, validation, measurement and chemometric analysis processes. Given the fact that Raman spectroscopy is a modern and innovative field, the standardisation processes are complex and constantly evolving. Despite the seemingly high number of existing standards, the standardisation landscape is incomplete and has not been modernised according to the developments in Raman spectroscopy techniques in recent years. This is evident by the lack of protocols for numerous areas as well as by the fact that some of the existing standards have not been updated to reflect the advances in the technique. Therefore, it is important for the Raman community to actively engage in and contribute to a modernisation process that will result in updating exiting and introducing new terms, protocols and guides. Indeed, the development of optimised common standards would be extremely beneficial and would further foster the development and application of Raman spectroscopy techniques, most notably those of surface enhanced Raman spectroscopy and low-resolution portable analysers.

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Section: Discussionmentioning

confidence: 99%

Review of Existing Standards, Guides, and Practices for Raman Spectroscopy

Ntziouni

Thomson²,

Xiarchos

et al. 2022

Appl Spectrosc

View full text Add to dashboard Cite

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“…However, this approach can be challenging to integrate in most Raman spectroscopy acquisition workflows due to the requirement of additional equipment and the difficulty of positioning the calibration lamp. 40 Instead, an alternate method for calibrating Raman instruments (e.g., correct for filter, detector etaloning, and quantum efficiency effects 41 ) is based on measurement on a standard reference material (SRM) calibrated and manufactured by NIST (SRM-2241 for 785-nm excitation). 35 Because this method does not involve the use of additional instruments (irradiance source), it is better suited for use in clinical environments where time and space are limited.…”

Section: Methodsmentioning

confidence: 99%

“…Typical methods involve measuring using a spectrum from a reference calibration light source of known emission profile to recover the instrument response function (IRF). However, this approach can be challenging to integrate in most Raman spectroscopy acquisition workflows due to the requirement of additional equipment and the difficulty of positioning the calibration lamp 40 . Instead, an alternate method for calibrating Raman instruments (e.g., correct for filter, detector etaloning, and quantum efficiency effects 41 ) is based on measurement on a standard reference material (SRM) calibrated and manufactured by NIST (SRM-2241 for 785-nm excitation) 35 .…”

Section: Methodsmentioning

confidence: 99%

Open-sourced Raman spectroscopy data processing package implementing a baseline removal algorithm validated from multiple datasets acquired in human tissue and biofluids

et al. 2023

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.SignificanceStandardized data processing approaches are required in the field of bio-Raman spectroscopy to ensure information associated with spectral data acquired by different research groups, and with different systems, can be compared on an equal footing.AimAn open-sourced data processing software package was developed, implementing algorithms associated with all steps required to isolate the inelastic scattering component from signals acquired using Raman spectroscopy devices. The package includes a novel morphological baseline removal technique (BubbleFill) that provides increased adaptability to complex baseline shapes compared to current gold standard techniques. Also incorporated in the package is a versatile tool simulating spectroscopic data with varying levels of Raman signal-to-background ratios, baselines with different morphologies, and varying levels of stochastic noise.ResultsApplication of the BubbleFill technique to simulated data demonstrated superior baseline removal performance compared to standard algorithms, including iModPoly and MorphBR. The data processing workflow of the open-sourced package was validated in four independent in-human datasets, demonstrating it leads to inter-systems data compatibility.ConclusionsA new open-sourced spectroscopic data pre-processing package was validated on simulated and real-world in-human data and is now available to researchers and clinicians for the development of new clinical applications using Raman spectroscopy.

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“…In 1928, C. V. Raman experimentally confirmed the existence of Raman scattering, for which he was awarded the Nobel Prize in Physics in 1930 138 . The ability of Raman spectroscopy to calculate the composition and structure of analytes based on vibrational information has led to its subsequent widespread use in biomedicine, analytical chemistry, and other fields 139–143 . The weak Raman scattering cross section leads to inefficient Raman scattering, which limits its application in analysis practice due to the lack of sufficient signal intensity.…”

Section: Sers‐based Biosensingmentioning

confidence: 99%

Optical biosensing systems for a biological living body

Yan

Guo

Wang

et al. 2023

VIEW

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With the development of technology, biosensors are increasingly used in the biomedical field. Due to the high sensitivity of optical signals, good stability, high signal‐to‐noise ratio, and different spectral characteristics of different analytes, optical biosensors can achieve direct and real‐time detection of analytes with high specificity compared with traditional analytical techniques. In view of the rapid development of optical biosensors for in vivo applications and their promising future, we have attempted to summarize the working principles and recent advances in application in humans, with the aim of helping readers to gain an in‐depth and detailed understanding of optical biosensors developed in recent years for applications in the biological living body. In this review, we focus on some of the current widely used and promising optical biosensors, including colorimetric biosensors, fluorescence biosensors, and surface‐enhanced‐Raman‐scattering‐based biosensors. The working principles for each type of optical biosensor are concisely described and the application of the biosensor in the living body is summarized by category. We conclude by looking at the prospects of in vivo applications of optical biosensors.

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Evaluation of standardized performance test methods for biomedical Raman spectroscopy

Cited by 9 publications

References 26 publications

Review of Existing Standards, Guides, and Practices for Raman Spectroscopy

Review of Existing Standards, Guides, and Practices for Raman Spectroscopy

Open-sourced Raman spectroscopy data processing package implementing a baseline removal algorithm validated from multiple datasets acquired in human tissue and biofluids

Optical biosensing systems for a biological living body

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