Evaluation of sperm subpopulation structure in relation to in vitro sperm–oocyte interaction of frozen-thawed semen from Holstein bulls

Ferraz, Marcia de Almeida Monteiro Melo; Morató, Roser; Yeste, Marc; Arcarons, N.; Martínez, Ana Isabel Peña; Tamargo, Carolina; Hidalgo, C.O.; Muíño, R.; Mogas, T.

doi:10.1016/j.theriogenology.2014.01.033

Cited by 50 publications

(55 citation statements)

References 41 publications

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“…Individual differences on sperm quality may explain that variation over LIN, STR and WOB after PG selection in frozen semen of cat. In this context, semen samples containing high LIN, STR and WOB values are related to successful ZP binding (Ferraz et al, 2014), which may influence subsequent fertility. Further analyzes are needed to know the effect of sperm kinetic characteristics over fertilization in cats.…”

Section: Discussionmentioning

confidence: 99%

Effect of Sperm Selection Techniques in Frozen/Thawed Cat Spermatozoa on Sperm Motility Analyzed by CASA System

2017

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CHEUQUEMÁN, C.; SÁNCHEZ, R. & RISOPATRÓN, J.Effect of sperm selection techniques in frozen/thawed cat spermatozoa on sperm motility analyzed by CASA system. Int. J. Morphol., 35(4):1495Morphol., 35(4): -1501Morphol., 35(4): , 2017. SUMMARY:Freeze/thawing process reduces sperm survival and fertilizing ability of cat spermatozoa, with sperm motility being the most sensitive sperm parameter altered, due to cryo-damage. In this context, swim-up and density gradient processing methods can help to recover high motile and normal spermatozoa. Maximizing the use of frozen semen sample is essential, especially in endangered felids or high value cats in which sample size, number of samples or access to semen collection is reduced. To our knowledge, there is no previous report describing an in depth analysis of sperm motility improvement, after sperm selection techniques in frozen cat semen. Accordingly, we evaluated the effect of percoll gradient (PG) and swim up (SU) sperm selection techniques on sperm motility parameters and sperm recovery rate in frozen/thawed spermatozoa of domestic cat. Next, we evaluated the individual effect of the cat over sperm motility after PG sperm selection of frozen/thawed spermatozoa. SU and PG improved significantly all sperm motility parameters of frozen/thawed cat spermatozoa compared to simple washing. However, PG allows better sperm recovery from the original frozen sample and works mostly homogeneously among individual cats. This new information could help to maximize the use of frozen semen in endangered felids or high value domestic cats for its subsequent application on in vitro fertilization and artificial insemination.

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Section: Discussionmentioning

confidence: 99%

Effect of Sperm Selection Techniques in Frozen/Thawed Cat Spermatozoa on Sperm Motility Analyzed by CASA System

2017

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“…Sperm subpopulations, commonly devised either by principle component analysis or clustering, have been known to have a positive and significant correlation on the ejaculate quality and fertilizing ability in bulls both in vitro and in vivo [4][5][6]. The cryopreservation process had also been found out to significantly modify the distribution of spermatozoa within subpopulations, and the magnitude of the subpopulation in fresh ejaculates was positively correlated with their resistance to cryopreservation.…”

Section: Identification Of Sperm Subpopulations In Water Buffalo Ejacmentioning

confidence: 99%

“…The cryopreservation process does not only induce a loss of sperm viability, but also impair the functionality of the surviving spermatozoa, which accounts for the lower fertilizing capacity of the frozen-thawed semen [6]. Realizing the effects, the sperm subpopulations are now being used to identify the quality of the semen and its potential fertilizing ability [4].…”

Section: Identification Of Sperm Subpopulations In Water Buffalo Ejacmentioning

confidence: 99%

Sperm subpopulations, buffalo semen, sperm kinematics, cryopreservation, computer assisted sperm analyzer.

Maylem¹,

Leoveras²,

Venturina³

et al. 2017

JAST-A

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Abstract:This study aimed to identify and characterize the sperm subpopulations existing in water buffalo semen using a computer assisted sperm analyzer (CASA), as well as assess the effects of cryopreservation on the sperm subpopulation structure and evaluate bull variability. The semen of eight Bulgarian Murrah bulls was collected by four times in an interval of one week each. The semen was cryopreserved following a standard protocol and sperm kinematics was assessed. Clustering methods were applied to individual sperms, forming two significantly different (P < 0.05) subpopulations (P 1 and P 2 ). Subpopulation P 1 represents those spermatozoa that moved most rapidly and progressively (46.29%), and subpopulation P 2 includes spermatozoa with relatively low velocity or poorly motile but with high progressiveness (53.41%). There was a decline on the population of P 1 sperms from fresh (52.52%), pre-freeze (45.73%) to post-thaw (35.17%) stages and significant difference on the sperm kinematics between P 1 and P 2 . A significant decline in the values of distance, velocity and amplitude of lateral head (ALH) parameters were observed at post-thaw stage, while an increase was observed on trajectory and beat cross frequency (BCF) kinematics. Values of sperm kinematics were also significantly different (P < 0.05) among all bulls. The frequency distribution of spermatozoa on both subpopulations P 1 and P 2 was quite similar for all bulls in pre-freeze and post-thaw stages, but with significant (P < 0.05) variability on fresh stage. Bulls with the highest maintained frequency of P 1 sperms are denoted as good freezer bulls. In sum, kinematic characterization of water buffalo sperm and clustering into subpopulation enable to identify bulls that are more resistant to cryopreservation and production of quality semen for genetic propagation.

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“…Se ha reconocido que la capacidad fecundante de los espermatozoides puede ser evaluada mediante el ensayo de unión a zona pelúcida, que mide el número de espermatozoides que se unen a la zona pelúcida del ovocito; además esta prueba integra las diversas funciones espermáticas que son necesarias para la interacción con el gameto femenino (Meyers et al, 1995;Oehninger et al, 1997;Fazeli et al, 1997;Liu y Baker, 2000;Oehninger et al, 2000;Gonzales et al, 2001;Oehninger et al, 2003;Lamb, 2010;Franken y Oehninger, 2012;Ferraz et al, 2014;Oehninger et al, 2014). Por este motivo, en el presente trabajo se realizó el ensayo en zona pelúcida del efecto del fluido folicular de alpaca en la interacción gamética de esta especie.…”

Section: Introductcionunclassified

EFECTO DEL FLUIDO FOLICULAR SOBRE LA CAPACIDAD DE UNIÓN A ZONA PELÚCIDA DE ESPERMATOZOIDES EPIDIDIMARIOS DE ALPACA (Vicugna pacos)

Bravo¹,

Rubén²,

Valdivia³

2017

SPERMOVA

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Follicular fluid is the liquid fraction that surrounds the oocyte during its growth and maturation in the ovary. The components of the follicular fluid can favor the functionality of the sperm. In the present research, the binding to the zona pellucida of epididymal spermatozoa of alpaca incubated with follicular fluid was studied. In the experiment a control group and a treatment group were used. The sperm from the control group and the treatment were incubated with zona pellucidae. The incubation of the treatment group was performed in the presence of follicular fluid at a concentration of 30%; while the incubation of the control group occurred in the absence of follicular fluid. It was observed that a greater number of spermatozoa joined the zona pellucida in the group incubated in the presence of follicular fluid. It was found that there were statistical differences between treatment and control. It is concluded that alpaca follicular fluid enhances the in vitro binding of spermatozoa to the zona pellucida of this species.Keywords: Alpaca, follicular fluid, zona pellucida, epididymal sperm, gametic interaction. RESUMENEl fluido folicular es la fracción líquida que rodea al ovocito durante su crecimiento y maduración en el ovario. Los componentes del fluido folicular pueden favorecer la funcionalidad de los espermatozoides. En el presente trabajo se ha estudiado la unión a zona pelúcida de espermatozoides epididimarios de alpaca incubados con fluido folicular de esta especie. En el experimento se utilizó un grupo control y un grupo tratamiento. Los espermatozoides del grupo control y el tratamiento fueron incubados con zonas pelúcidas. La incubación del grupo tratamiento se realizó en presencia de fluido folicular a una concentración de 30%; mientras que la incubación del grupo control se dio en ausencia de fluido folicular. Se observó que un mayor número de espermatozoides se unían a la zona pelúcida en el grupo incubado en presencia de fluido folicular. Se encontró que había diferencias estadísticas entre el tratamiento y el control. Se concluye que el fluido folicular de alpaca favorece la unión in vitro de espermatozoides a la zona pelúcida de esta especie.Palabras clave: Alpaca, fluido folicular, zona pelúcida, espermatozoides epididimarios, interacción gamética.

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Evaluation of sperm subpopulation structure in relation to in vitro sperm–oocyte interaction of frozen-thawed semen from Holstein bulls

Cited by 50 publications

References 41 publications

Effect of Sperm Selection Techniques in Frozen/Thawed Cat Spermatozoa on Sperm Motility Analyzed by CASA System

Effect of Sperm Selection Techniques in Frozen/Thawed Cat Spermatozoa on Sperm Motility Analyzed by CASA System

Sperm subpopulations, buffalo semen, sperm kinematics, cryopreservation, computer assisted sperm analyzer.

EFECTO DEL FLUIDO FOLICULAR SOBRE LA CAPACIDAD DE UNIÓN A ZONA PELÚCIDA DE ESPERMATOZOIDES EPIDIDIMARIOS DE ALPACA (Vicugna pacos)

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