Evaluation of sperm functional attributes in relation to in vitro sperm-zona pellucida binding ability and cleavage rate in assessing frozen thawed buffalo (Bubalus bubalis) semen quality

Selvaraju, Sellappan; Ravindra, J.P.; Ghosh, Jyotirmoy; Gupta, P. S. P.; Suresh, K

doi:10.1016/j.anireprosci.2007.05.005

Cited by 77 publications

(72 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results obtained in the present study were higher than the value of 6.70% of sperm cells with high mitochondrial membrane potential reported by Selvaraju et al (2008) in buffalo semen.…”

Section: Mitochondrial Membrane Potential Of Sperm Cellscontrasting

confidence: 55%

“…The staining procedure was conducted according to the protocol recommended by the manufacturer. Mitochondrial membrane potential was assessed by using JC-1 (5, 5 ′ , 6, 6 ′ -tetrachloro-1, 1 ′ , 3, 3 ′ -tetraethylbenzimidazolylcarbocyanine iodide) and plasmalemma integrity was assessed by carboxy fluoresin diacetate (CFDA) and propidium iodide (PI) as described by Selvaraju et al (2008).…”

Section: Assessment Of In Vitro Sperm Charactersmentioning

confidence: 99%

“…In vitro sperm characters were evaluated at immediate post thaw, 60, 120 and 180min post-thaw incubation (Bollwein et al 2008). Motility analysis was conducted using a computer-assisted semen analyser (Sperm Class Analyzer, Microptic, Barcelona, Spain) as described by Selvaraju et al (2008). Functional membrane integrity was assessed using osmotic resistance test (hypo-osmotic swelling test) by incubating an aliquot (100 µl) of semen sample with 1 ml of 150 mOsm hypo-osmotic and 300 mOsm iso-osmotic (control) solutions at 37°C for 30 min (Jeyendran et al 1984).…”

Section: Assessment Of In Vitro Sperm Charactersmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of bull semen for fertility-associated protein, in vitro characters and fertility

Karunakaran

Devanathan

2016

Journal of Applied Animal Research

View full text Add to dashboard Cite

Proteins present in the seminal plasma and sperm influence sperm function and fertilization. The present study was carried out to screen breeding bull semen samples for the presence of fertilityassociated 28-30 kDa heparin-binding protein (HPB) and its effect on in vitro sperm characters and fertility. Semen samples were collected from 22 breeding bulls and the sperm proteins were extracted by Triton X detergent extraction method. HBPs were eluted, and the molecular weight of the proteins was assessed by discontinuous sodium dodecyl sulphate polyacrylamide gel elecrophoresis. Based on the presence/absence 28 kDa HBPs, bulls were categorized into group I and group II. Frozen semen samples were evaluated for in vitro sperm characters at immediate post thaw, 60, 120 and 180 min post-thaw incubation. To assess the field fertility of the bulls, 50 frozen semen straws/bull were used for insemination. Results indicated that only 50% of the bulls screened had 28-30 kDa HBPs in their sperm. Bulls positive for fertility-associated protein had better in vitro sperm characters, better protection against oxidative stress, readily underwent capacitation induction by heparin and had 13% higher conception than the bulls lacking the protein. So, it can be concluded that the bulls positive for of 28-30 kDa HBPs in sperm had higher chance of fertility and screening for its presence can be included in the regular breeding soundness examination for selection of bulls. ARTICLE HISTORY

show abstract

Section: Mitochondrial Membrane Potential Of Sperm Cellscontrasting

confidence: 55%

Section: Assessment Of In Vitro Sperm Charactersmentioning

confidence: 99%

Section: Assessment Of In Vitro Sperm Charactersmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of bull semen for fertility-associated protein, in vitro characters and fertility

Karunakaran

Devanathan

2016

Journal of Applied Animal Research

View full text Add to dashboard Cite

show abstract

“…Thus, an absence of these proteins might affect fertility55. Spermatozoal motility is correlated with mitochondrial function56, which regulates energy generation, apoptosis and calcium homeostasis. In this study, COX5 A and COXI1 transcripts, which are associated with mitochondrial function, were observed in the spermatozoa along with their respective proteins.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and functional significance of the transcriptome in bovine (Bos taurus) spermatozoa

Selvaraju

Parthipan

Somashekar

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Mammalian spermatozoa deliver various classes of RNAs to the oocyte during fertilization, and many of them may regulate fertility. The objective of the present study was to determine the composition and abundance of spermatozoal transcripts in fresh bull semen. The entire transcriptome of the spermatozoa from bulls (n = 3) was sequenced using two different platforms (Ion Proton and Illumina) to identify the maximum number of genes present in the spermatozoa. The bovine spermatozoa contained transcripts for 13,833 genes (transcripts per million, TPM > 10). Both intact and fragmented transcripts were found. These spermatozoal transcripts were associated with various stages of spermatogenesis, spermatozoal function, fertilization, and embryo development. The presence of intact transcripts of pregnancy-associated glycoproteins (PAGs) in the spermatozoa suggest a possible influence of sperm transcripts beyond early embryonic development. The specific regions (exon, intron, and exon-intron) of the particular spermatozoal transcripts might help regulate fertilization. This study demonstrates that the use of two different RNA-seq platforms provides a comprehensive profile of bovine spermatozoal RNA. Spermatozoal RNA profiling may be useful as a non-invasive method to delineate possible causes of male infertility and to predict fertility in a manner that is more effective than the conventional methods.

show abstract

“…The mean of the three estimations was used as the final motility score [17]. 6-carboxyfluorescein diacetate (CFDA) and propidium iodide (PI) staining was done to assess the sperm viability as described by Selvaraju et al [18]. Briefly, semen sample was incubated with phosphate buffered saline (PBS) based CFDA/PI staining solution at 37°C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Absolute copy number differences of Y chromosomal genes between crossbred (Bos taurus × Bos indicus) and Indicine bulls

Mukherjee

Dass

et al. 2013

J Animal Sci Biotechnol

View full text Add to dashboard Cite

BackgroundThe Y chromosome in mammal is paternally inherited and harbors genes related to male fertility and spermatogenesis. The unique intra-chromosomal recombination pattern of Y chromosome and morphological difference of this chromosome between Bos taurus and Bos indicus make it an ideal model for studying structural variation, especially in crossbred (Bos taurus × Bos indicus) bulls. Copy Number Variation (CNV) is a type of genomic structural variation that gives information complementary to SNP data. The purpose of this study was to find out copy number differences of four Y chromosomal spermatogenesis-related candidate genes in genomic DNA of crossbred and purebred Indicine bulls.ResultFour Y chromosomal candidate genes of spermatogenesis namely, sex determining gene on Y chromosome (SRY), DEAD box polypeptide 3-Y chromosome (DDX3Y), Ubiquitin specific peptidase 9, Y-linked (USP9Y), testis-specific protein on Y chromosome (TSPY) were evaluated. Absolute copy numbers of Y chromosomal genes were determined by standard curve-based quantitative real time PCR. Copy numbers of SRY and TSPY genes per unit amount of genomic DNA are higher in crossbred than Indicine bulls. However, no difference was observed in DDX3Y and USP9Y gene copy numbers between two groups.ConclusionThe present study demonstrates that the structural organization of Y chromosomes differs between crossbred and Indicine bulls which are reproductively healthy as observed from analysis of semen attributes. The absolute copy numbers of SRY and TSPY genes in unit mass of genomic DNA of crossbred bulls are significantly higher than Indicine bulls. No alteration in absolute copies of DDX3Y and USP9Y gene was found between the genome of crossbred and Indicine bulls. This study suggests that the DDX3Y and USP9Y are likely to be single copy genes in the genome of crossbred and Indicine bulls and variation in Y chromosome length between crossbred and Indicine bulls may be due to the copy number variation of SRY gene and TSPY array.

show abstract

Evaluation of sperm functional attributes in relation to in vitro sperm-zona pellucida binding ability and cleavage rate in assessing frozen thawed buffalo (Bubalus bubalis) semen quality

Cited by 77 publications

References 31 publications

Evaluation of bull semen for fertility-associated protein, in vitro characters and fertility

Evaluation of bull semen for fertility-associated protein, in vitro characters and fertility

Occurrence and functional significance of the transcriptome in bovine (Bos taurus) spermatozoa

Absolute copy number differences of Y chromosomal genes between crossbred (Bos taurus × Bos indicus) and Indicine bulls

Contact Info

Product

Resources

About