Evaluation of specific marker CK13 and CK10/13 combined with APM staining for the diagnosis of amniotic fluid embolism and aspiration

Wang, Jinjie; Lai, Qian; Pan, Hongyang; Sun, Dongdong; Yu, Chun-feng; Zhang, Wei; Chen, Jian; Ma, Lu; Ling, Li; Ren, Zhimin

doi:10.1016/j.forsciint.2014.02.032

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…In previous reports, AFA was present in 8% to 20% of all deliveries, especially in term and postterm infants, while the mortality rate reaches 39.3% in MAS and 13.5% in pure AFA. [22] The risk factors of asphyxia include mother's disease associated with inadequate oxygenation of blood, reduction of blood flow from the placenta to the mother's body, inadequate blood flow from the placenta to the fetal body, failure of gas exchange in the placenta, and conditions in the fetus associated with increased demands for oxygen. About 90% of the causes of asphyxia occurs prenatally and during labor.…”

Section: Discussionmentioning

confidence: 99%

Fetal, neonatal, and infant death in central China (Hubei)

Deng

Wang

Zhou³

et al. 2019

Medicine

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Data based on forensic autopsy in neonates and infants in China are rare in the literature. The purpose of this study is to evaluate the characteristics of fetal, neonatal, and infant death and to determine the main cause of death among them. A retrospective analysis of fetal and infant forensic autopsies referred to the Tongji Forensic Medical Center (TFMC) in Hubei, central China, during a 16-year period between January 1999 and December 2014, was performed. In this period, there were 1111 males and 543 females; the total male-to-female ratio (MFR) was 2.05:1. There were 173 fetal and infant autopsies conducted, comprised of 43 fetal, 84 neonatal (<28 days) and 46 infant (4 weeks to 1 year) cases. The annual case number ranged from 5 in 2004 to 18 in 2014 (annual mean of 10.8). MFR was 1.75:1. About 94% of these deaths (163/173) resulted from natural causes, 6 cases (3.5%) were accidental deaths, and 4 (2.3%) resulted from homicide (4 abandoned babies). Among fetuses, the most common causes of death were placental and umbilical cord pathologies (28%, 12/43), followed by intrapartum asphyxia resulting from amniotic fluid aspiration (AFA) or meconium aspiration syndrome (MAS) (18.6%, 8/43), congenital malformation (14%, 6/43), and intrapartum infection (9.3%, 4/43). A majority of neonatal deaths (66.7%, 56/84) died within 24 hours of birth. The main causes of neonatal death were asphyxia resulting from AFA, MAS, or hyaline membrane disease, and congenital malformation. The main causes of infant (1–12 months) death were infectious diseases, including pneumonia, meningitis, and viral brainstem encephalitis. This study was the 1st retrospective analysis of autopsies of fetal, neonatal, and infant death in TFMC and central China. We delineate the common causes of early demise among cases referred for autopsy, and report a male preponderance in this population. Our data observed that placental and/or umbilical cord pathology, asphyxia due to AFA, and/or MAS, and pneumonia were the leading causes of fetal, neonatal, and infant death, respectively. And it can inform clinical practitioners about the underlying causes of some of the most distressing cases in their practices.

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Section: Discussionmentioning

confidence: 99%

Fetal, neonatal, and infant death in central China (Hubei)

Deng

Wang

Zhou³

et al. 2019

Medicine

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“…Single immunochemistry staining was applied in diagnosing specific trauma due to its sensitivity in identifying the target protein, such as the IHC staining of tubular myoglobin aiding in the verification of crush syndrome in judicial autopsy [37]. The positive expression of CD31 and cytokeratins in fetal lung tissue can help forensic pathologists definitely diagnose amniotic fluid embolism more easily compared with routine hematoxylin eosin detection [30]. It was known that morphological features are often absent in some negative autopsies.…”

Section: Immunohistochemistry (Ihc) Assaymentioning

confidence: 99%

The Role of Protein Degradation in Estimation Postmortem Interval and Confirmation of Cause of Death in Forensic Pathology: A Literature Review

Huang,

Zhao,

Liu

et al. 2024

IJMS

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It is well known that proteins are important bio-macromolecules in human organisms, and numerous proteins are widely used in the clinical practice, whereas their application in forensic science is currently limited. This limitation is mainly attributed to the postmortem degradation of targeted proteins, which can significantly impact final conclusions. In the last decade, numerous methods have been established to detect the protein from a forensic perspective, and some of the postmortem proteins have been applied in forensic practice. To better understand the emerging issues and challenges in postmortem proteins, we have reviewed the current application of protein technologies at postmortem in forensic practice. Meanwhile, we discuss the application of proteins in identifying the cause of death, and postmortem interval (PMI). Finally, we highlight the interpretability and limitations of postmortem protein challenges. We believe that utilizing the multi-omics method can enhance the comprehensiveness of applying proteins in forensic practice.

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“…Components of amniotic fluid can be determined with hematoxylin-and-eosin, alcian-phloxine-martius yellow (testing for mucin), cytokeratin AE1/AE3 (for squamous cells), ZnCp-1 (for meconium), and C5aR stain (for complement activation) [34][35][36]. It has been noted, however, that only about 50% of patients with AFE had detectable fetal debris present in maternal circulation [6,37].…”

Section: Diagnostic Considerationsmentioning

confidence: 99%

Amniotic fluid embolism

Balinger¹,

Lam

Hon³

et al. 2015

Current Opinion in Obstetrics &Amp; Gynecology

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Although AFE cannot be prevented, early diagnosis and intervention may lead to better outcomes for both the mother and the fetus. Clinical suspicion, traditional laboratory data, or intravascular cellular debris (demonstrated only in 50% of patients) are insufficient to make a definitive diagnosis of AFE. An evolving array of novel biomarkers may help differentiate AFE from other conditions, but none of them currently provide sufficient 'early warning' ability to make real-time impact on diagnosis and/or treatment of AFE.

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Evaluation of specific marker CK13 and CK10/13 combined with APM staining for the diagnosis of amniotic fluid embolism and aspiration

Cited by 4 publications

References 31 publications

Fetal, neonatal, and infant death in central China (Hubei)

Fetal, neonatal, and infant death in central China (Hubei)

The Role of Protein Degradation in Estimation Postmortem Interval and Confirmation of Cause of Death in Forensic Pathology: A Literature Review

Amniotic fluid embolism

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