Evaluation of Some Therapies to Eliminate Potato Y Potyvirus from Potato Plants

Mahmoud, S. Y. M.; Hosseny, M. H.; Abdel-Ghaffar, Mamdouh H.

doi:10.3923/ijv.2009.64.76

Cited by 30 publications

(29 citation statements)

References 30 publications

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“…e results showed that the treatment with 15 mA for 10 min and combined with meristem 100 µm in length gave the highest number of PVY virus-free plantlets (93% in Binella and 87% in Burren), but e rate of virus-free plantlets decreased to 62% in Binella and 57% in Burren when the treatment was 15 mA for 5 min combined with meristem (300 µm) ( Table 4 and Table 5). Present results agreed with the ndings of (Mahmoud et al 2009) showing that high electric current (15 mA) for (10 min) has more a ectivity for virus elimination under in vitro condition. E ciency of 60 to 100% were reported for electrotherapy in the production of virus- Table 3 ) were added to the meristem culture media.…”

supporting

confidence: 92%

Evaluation of some therapies and meristem culture to eliminate Potato Y potyvirus from infected potato plants

Al-Maarri

Massa

Al-Biski

2012

Plant Biotechnology

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supporting

confidence: 92%

Evaluation of some therapies and meristem culture to eliminate Potato Y potyvirus from infected potato plants

Al-Maarri

Massa

Al-Biski

2012

Plant Biotechnology

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“…Thermotherapy, even in association with other techniques such as tissue culture, represents the most fre- Goussard & Wiid, 1992;Popescu et al, 2003Popescu et al, , 2010Gambino et al, 2006Gambino et al, , 2010Gribaudo et al, 2006;Borroto-Fernández et al, 2009;Youssef et al, 2009Ramos & Zamora, 1999D'Onghia et al, 2001;Golino et al, 2007;Quainoo et al, 2008Fitch et al, 2001Parmessur et al, 2002;Ramgareeb et al, 2010Ebi et al, 2000Ayabe & Sumi, 2001;Ramírez-Malagón et al, 2006Truskinov & Rogozina, 1997Mahmoud et al, 2009Wangai & Bock, 1996Morris et al, 1997;S edivá et al, 2006;Kumar et al, 2009;Kabir et al, 2010 quently used technique for sanitation over the last 20 years, while applications of methods such as chemotherapy and tissue culture represent the main topic in plant sanitation research.…”

Section: Comparison Of Techniquesmentioning

confidence: 99%

Review. Elimination of viruses in plants: twenty years of progress

Panattoni

Luvisi

Triolo

2013

Span. j. agric. res.

144

112

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To shed light on trends about elimination of viruses from plants, a bibliographic research was conducted to identify thermotherapy, chemotherapy and tissue culture trials published from 1991 through 2010. Among woody plants, grapevine, apple and peach are the most frequent targets of sanitation protocols because their health status is strictly regulated. Even if thermotherapy represents the preferred method for the host, grapevine viruses can also be eliminated with chemotherapy and tissue culture; apple viruses respond to chemotherapy as well. Although a similar trend was reported among herbaceous plants, chemotherapy was the most frequently used technique in potato. With regard to virus, thermotherapy was successfully applied against viruses belonging to 13 families and an unassigned genus. Instead, chemotherapy and tissue culture techniques eradicated viruses belonging to fewer families (nine). An interpretation of thermotherapy effects considers the new metabolic “pathways” triggered by the natural antiviral response emitted by the infected plant, with particular reference to virus-induced gene silencing. With regard to chemotherapy, several groups of antiviral drugs belong to inosine monophosphate dehydrogenase inhibitors, S-adenosylhomocysteine hydrolase inhibitors, neuraminidase inhibitors. Tissue culture, usually adopted to regenerate plantlets in biotechnological breeding programs, represents the less used tool for eliminate viruses from plants.

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“…T h e a p i c a l m e r i s t e m s i n v i t r o c u l t u r e + b [22] ++ a [23] + a [37] ++ [8] ++ [8] ++ [11,23,28,41]  а [11,23] ++ а [11,17]  а [11,23] ++ а [17] ++ а [23] ++++ а [11,38] ++ а [23] +++ а [11] Thermotherapy and meristems culture ++ c [22] ++++ а [23] ++++ а [8,42] ++++ а [8,42] ++ а [24] ++++ а [23,42] Chemotherapy and meristems culture ++++ а [25] ++++ а, c [26] ++ а, c [26] ++ а, c [26] +++ а [25] ++++ а, c [26] Thermo-, chemotherapy and meristems culture +++ а [27] +++ а [27] ++++ а [37] +++ а [27] +++ а [27] Thermo-, chemo-, electrotherapy and meristems culture +++ а [9] +++ а [10] ++++ а, c [26] ++++ c [12] As it was shown by ELISA for the original plants from the field collection (data not provided) and RT-PCR analysis of derived micro-plants of the same clones, all the samples were affected by virus...…”

Section: The Effectiveness Of Various Sanitation Methods For Potato Cmentioning

confidence: 99%

“…During the cryotherapy in liquid nitrogen (196 С) only meristem cells potentially free from viruses remain viable, and hydrated cells outside the meristem zone die [8]. The method of electrotherapy is based on plant tissues exposure to electrical current, resulting in the degradation of the viral nucleoprotein and the loss of virulence [9,10].…”

mentioning

confidence: 99%

ERADICATION OF VIRUSES IN MICROPLANTS OF THREE CULTIVATED POTATO SPECIES (Solanum tuberosum L., S. phureja Juz. & Buk., S. stenotomum Juz. & Buk.) USING COMBINED THERMO-CHEMOTHERAPY METHOD

Antonova¹,

Апаликова²,

Ukhatova³

et al. 2017

S-h. biol.

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A b s t r a c tA modified method of combined chemo-thermotherapy for eradication the most damaging viruses in potato plants is proposed. The materials for study included 91 clones of 85 accessions of three South-American cultivated species Solanum phureja (18 accessions), S. stenotomum (26 accessions), S. tuberosum (S. tuberosum ssp. andigenum and S. tuberosum ssp. tuberosum) (41 accessions) from the VIR field collection (N.I. Vavilov All-Russian Institute of Plant Genetic Resources). The results of ELISA for 91 clones from the field collection and RT-PCR analysis of the corresponding microplants, when introducing clones into in vitro culture, have shown that all material was infected by viruses (potato viruses Y, X, S, M and potato leafroll virus -PVY, PVX, PVS, PVM and PLRV) to varying degrees. Single infections were detected in 25.3 % of the 91 clones, and the remaining clones were multi-infected and contained several viruses in various combinations. Based on the termo-and chemotherapy protocols as well as complex therapy used in leading potato genebanks (Leibniz Institute of Plant Genetics and Crop Plant Research -IPK, Germany; International Potato Centre -CIP, Peru), we have developed a modified method of combined therapy comprising three successive cycles of in vitro plant cultivation on Murashige and Skoog basal medium (MS) supplemented with ribavirin (30 mg/l) at 37 С during 4 weeks. Between the cycles, the microplants were incubated for 2 days at 26 С on the same medium with ribavirin. Each clone was tested for the presence of viruses by RT (revers transcription) PCR before and after the combined thermo-chemotherapy. In RT-PCR, 42 clones (46 %) were completely free from tested viruses, 44 % were free from some viruses, and 10 % of the clones were unable to improve. On the whole of 91 clones, PLRV was eliminated from 72.7 %, PVY from 71.4 %, PVM from 63.9 % and PVS from 57.4 % of microplants. Differences in elimination frequency between the viruses were not significant (р > 0.05). Also, there were no significant differences in elimination of different viruses between the accessions of different cultivated species. The difference of this modification from IPK protocols (Germany) lays in simultaneous effect of high temperature (37 C) and ribavirin (instead of sequential application), and, unlike the CIP (Peru) scheme, it comprises less steps.

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Evaluation of Some Therapies to Eliminate Potato Y Potyvirus from Potato Plants

Cited by 30 publications

References 30 publications

Evaluation of some therapies and meristem culture to eliminate Potato Y potyvirus from infected potato plants

Evaluation of some therapies and meristem culture to eliminate Potato Y potyvirus from infected potato plants

Review. Elimination of viruses in plants: twenty years of progress

ERADICATION OF VIRUSES IN MICROPLANTS OF THREE CULTIVATED POTATO SPECIES (Solanum tuberosum L., S. phureja Juz. & Buk., S. stenotomum Juz. & Buk.) USING COMBINED THERMO-CHEMOTHERAPY METHOD

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