Abstract:Isolation of DNA from environmental samples is a crucial step in microbial community analyses through molecular methods. The present study was conducted to evaluate a DNA extraction protocol from paddy soil with a comparison on quality, quantity and integrity of the isolated DNA and to determine the suitability of extracted DNA for downstream applications in microbial community analyses. Three protocols (i.e. PEG/NaCl, Mannitol/CTAB and Sodium Phosphate Buffer) used for the extraction of DNA from different typ… Show more
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