Evaluation of some morphological characteristics of spores of two species of microsporida by scanning electron microscope and freeze‐etching techniques

Liu, T. P.; Liu, H. J.

doi:10.1002/jmor.1051430306

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…One can use the number of polar filament coils to distinguish the Nosema species [65]. Nosema apis contains 30 to 44 polar filament coils [66][67][68], while N. ceranae and N. neumanni include 20 to 23 polar filament coils [69] and 10 to 12 polar filament coils [7], respectively. Our polar filament coil data allow us to confirm N. ceranae identity, given that the analyzed spores had 17 to 20 polar filament coils [69].…”

Section: Discussionmentioning

confidence: 99%

The Optimal Concentrations of Ethanolic-Extracted Propolis for a Reduction of Nosema ceranae Infection in Western Honey Bees, Apis mellifera

Naree,

Ellis,

Suwannapong

2024

Preprint

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Ethanolic extracts of propolis collected from Apis mellifera hives have been shown to reduce Nosema ceranae infection in honey bee workers. This study aimed to determine the propolis concentration that optimizes the control of N. ceranae infection in honey bee workers. Newly emerged worker bees were individually fed 2 μL of 50% sucrose solution (w/v) containing 105 N. ceranae spores per bee, and treated with 0%, 25%, 50%, and 75% ethanolic extracted propolis in 50% sucrose solution (v/v). The treated bees were maintained in cages at 34 ± 2˚C and 55 ± 5% RH throughout the study. The number of dead bees was counted and removed from the cages daily for 30 days to generate survival curves. The number of N. ceranae spores per bee (infectivity) was quantified using a hemocytometer, and the number of infected bees per hundred bees (infection rate) was determined. The survival rates of N. ceranae infected bees fed 25%, 50%, and 75% propolis (NP1, NP2, NP3, respectively) were significantly higher than those of infected bees not fed propolis (NO). Furthermore, the infectivity and infection rates of NP2 and NP3 groups were significantly lower than those of the NO group. The infectivity and infection rates of the NP1 group were not different than those of the NO group. Our data suggest that 50% and 75% propolis extracts are appropriate concentrations for treating N. ceranae infection in honey bees. Ethanolic extracted propolis can be an alternative treatment for N. ceranae infection in Western honey bees.

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Section: Discussionmentioning

confidence: 99%

The Optimal Concentrations of Ethanolic-Extracted Propolis for a Reduction of Nosema ceranae Infection in Western Honey Bees, Apis mellifera

Naree,

Ellis,

Suwannapong

2024

Preprint

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Nosema bombi,a microsporidian pathogen of the bumble beeBombus terrestris(L.)

McIvor

Malone

1995

New Zealand Journal of Zoology

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The developmental cycle and morphology of a microsporidian pathogen Nosema bombi Fantham & Porter 1914, of the bumble bee, Bombus terrestris, are described. In experimentally infected bees, spherical meronts were the first stages observed. These varied in size from 2.66 to 7.35 µm, and had 1-4 nuclei. Uninucleate, binucleate, and occasionally tetranucleate sporonts were oval or fusiform in shape, and measured 5.32-8.05 µm × 2.80-4.41 µm. Oval binucleate sporoblasts, measuring 4.64-5.78 µm × 3.03-3.94 µm, appeared in large numbers prior to spore morphogenesis. Spores varied in size from 4.20-5.39 µm × 2.13-3.50 µm in unstained preparations, and were significantly smaller than spores of the honey bee pathogen, Nosema apis (P < 0.001, n = 50). Spores developed primarily in the Malpighian tubules. Electron microscopic examination of spores showed the polar filament to have 14-18 coils, compared with N. apis which has 18-44.

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Evaluation of some morphological characteristics of spores of two species of microsporida by scanning electron microscope and freeze‐etching techniques

Cited by 2 publications

References 7 publications

The Optimal Concentrations of Ethanolic-Extracted Propolis for a Reduction of <em>Nosema ceranae</em> Infection in Western Honey Bees, <em>Apis mellifera</em>

The Optimal Concentrations of Ethanolic-Extracted Propolis for a Reduction of <em>Nosema ceranae</em> Infection in Western Honey Bees, <em>Apis mellifera</em>

Nosema bombi,a microsporidian pathogen of the bumble beeBombus terrestris(L.)

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