Evaluation of Some Inflammatory Cytokines Levels as A Marker for Diabetes

Abstract

From the different causes of death diabetes has an advanced rank in the first 10. So the aim of this study was to relate between some pro-inflammatory cytokines (TNF-α, IL-6, and TGF-β1) and infection with diabetes as biomarkers for infection. Forty male Wister albino Streptozotocin (STZ) induced diabetic rats were used as a model for estimation of the related cytokines. The animals were divided in two groups, the first group was given a placebo, whereas the second was given Streptozotocin at a single dose of 70 mg/kg body weight to induce diabetes mellitus. Each rat's blood glucose level was measured 72 hours later. Diabetes was successfully induced in rats with blood glucose levels greater than 300 mg/dl. After 7 days, all groups were sacrificed and immunological responses, tumor necrosis factor-alpha (TNF-α), interleukin (IL-6), and transforming growth factor (TGF-β1) were measured. Glucose levels were shown to be significantly higher in the group that was given Streptozotocin at a single dose of 70 mg/kg body weight, with a percent change of 217.5% when compared to the control group. While in the diabetic group, there was also a large increase in IL-6, TNF-α and TGF-β1 levels, with a percent change of 82.24%, 77.7% and 74.5% respectively. From these results we can recommend these cytokines as a biomarkers for diabetes mellitus disease.

Evaluation of the relationship of cytokines concentrations tumor necrosis factor‐alpha, interleukin‐6, and C‐reactive protein in obese diabetics and obese non‐diabetics: A comparative study

“…31 Cytokines levels increase in diabetics. 32 Our discovery of a significant association between CRP with BMI and body fat is consistent with earlier findings indicating that BMI was the adiposity factor with the strongest correlation to CRP levels. 33 Studies differed in determining the cause.…”

“…T2DM is currently regarded as a chronic inflammatory disease 31 . Cytokines levels increase in diabetics 32 …”