Evaluation of Some Cereals, Plants and Tubers Through Protein Composition

Gorinstein, Shela; Jaramillo, Nicolas O.; Medina, Oscar J.; Rogriques, Walter A.; Tosello, Geraldo Antonio; Paredes‐López, Octavio

doi:10.1023/a:1020610424625

Cited by 24 publications

(3 citation statements)

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“…While A. hybridum 1004 and hypochondriacus v .1023 had main bands of Alb-2, most bands were observed in globulins for A. cruentus R104 ( Figure ). Soybean showed a major globulin band at 30 kDa and a low molecular weight band confirming findings of ( , ). Differences were observed between albumins (Alb-1 and Alb-2) and globulins of buckwheat and rice.…”

Section: Resultssupporting

confidence: 86%

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Identification and Differences of Total Proteins and Their Soluble Fractions in Some Pseudocereals Based on Electrophoretic Patterns

Drzewiecki

Delgado

Haruenkit

et al. 2003

J. Agric. Food Chem.

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Genetic diversity and relationships of 11 species and cultivars belonging to different Angiosperms families were examined using sodium dodecyl sulfate seed protein markers. The protein was resolved into 36 bands (for soybean), 41 (for quinoa), 35 (for buckwheat), and 28 to 39 bands of Amaranth species, respectively. All species and cultivars can be distinguished from each other. Soybean, quinoa, and buckwheat species had a characteristic protein pattern showing a high degree of polymorphism. The protein patterns of soybean were considerably different from other species. Amaranth species had similar seed protein electrophoretic profile. The similarity coefficients calculated on the basis of presence and absence of bands ranged from 0.08 to 0.97. Following the UPGMA algorithm of similarity coefficients, the examined species and varieties could be clustered into two similarity groups. Our results did not confirm the Tachtadzjan hypothesis that Polygonales (e.g., buckwheat) and Caryophyllales (e.g., quinoa and amaranth) are closely related. Our data rather indicate occurrence of significant genetic distance (similarity coefficients 0.05-0.10). Also, it is doubtful that amaranth and quinoa species are also closely related (similarity coefficients varied from 0.16 to 0.25). It seems that soybean, quinoa, buckwheat, and amaranth (as a genus) can be considered as phylogenetic distant taxa. Differences and similarities in the secondary structure were observed by circular dichroism spectra. Some similarity was found between these plants in their soluble protein fractions and amino acid composition. These plants can be a substitution of each other as well as for cereals.

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Section: Resultssupporting

confidence: 86%

“…Our results are not in full correspondence with others, who have shown that the amount of Alb-2 comprised about 30% of the total albumin and globulin content. Little or no measurable protein corresponding to the amaranth Alb-2 was extracted from seeds of buckwheat and quinoa ( , ).…”

Section: Resultsmentioning

confidence: 99%

Identification and Differences of Total Proteins and Their Soluble Fractions in Some Pseudocereals Based on Electrophoretic Patterns

Drzewiecki

Delgado

Haruenkit

et al. 2003

J. Agric. Food Chem.

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show abstract

“…Mezi nejmodernější varianty patří např. kapilární zónová elektroforéza s UV detekcí [28], chromatografie na reverzní fázi s fluorometrickou detekcí derivátů aminokyselin [29], kapilární zónová elektroforéza s elektrochemickou detekcí [30] a s následnou bezkontaktní vodivostní detekcí (contactless conductivity detection) [31], iontovýměnná chromatografie a elektroforéza s FT-IR [32,33], plynová chromatografie s chirální stacionární fází ke stanovení enantiomerů aminokyselin [34], insitu fotometrické fluorimetrické stanovení aminokyseliny tyrosinu [35], isokratické stanovení nederivatizovaných aminokyselin s ampérometrickou detekcí [36]. Dále sem patří metoda ke stanovení peptidů a aminokyselin s fluorescenčním činidlem 2-(9-karbazol)-Et chloroformat (CEOC) pomocí HPLC na reverzní fázi a hmotnostní spektrometrie [37], nová HPLC metoda k simultánnímu stanovení aminokyselin a aminů jako o-ftaldialdehyd derivátů [38], stanovení biogenních aminů metodou Heavy Atom Induced Phosphorescence (HAI-RTP) […”

Section: Přehled Metod Stanovení Aminokyselinunclassified