Evaluation of some antioxidant enzymes in lung carcinoma tissue

Güner, Gül; İşlekel, Hüray; Oto, Öxtekin; Hazan, E; Açıkel, Ünal

doi:10.1016/0304-3835(96)04226-7

Cited by 50 publications

(27 citation statements)

References 20 publications

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“…Thus, our results show that EA reduced the incidence of ACF in colonic epithelium. Increased oxy-radicals and loss of cellular homeostasis cause oxidative stress and lead to tumorigenesis (Guner et al, 1996). Oxidative stress, an imbalance in oxidant-antioxidant status, is characterized by an increase in lipid peroxidation and a decrease in antioxidant enzymes (Cerutti, 1994).…”

Section: Resultsmentioning

confidence: 99%

Chemomodulation of the antioxidative enzymes and peroxidative damage in the colon of 1,2‐dimethyl hydrazine‐induced rats by ellagicacid

Umesalma

Sudhandiran

2010

Phytotherapy Research

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Prevention of cancer remains a primary need and new chemopreventive agents must be developed for this purpose. Toward this goal, a chemopreventive study was conducted to evaluate the potential effect of ellagic acid (EA) against 1,2-dimethylhydrazine (DMH-) induced colon carcinogenesis in experimental rats. Rats were administered with DMH (20mg/kg body weight) subcutaneous injection once a week for 15 weeks and were supplemented with EA (60 mg/kg body weight/day orally). In the present study, the efficacy of EA on the formation of aberrant crypt foci (ACF), levels of lipid peroxidation (LPO) and activities of enzymic and non-enzymic antioxidants in DMH-induced colon-cancer-bearing rats were assessed. After the experimental period, frequency of ACF, levels of LPO were found to be increased, whereas a significant decrease in the activities of enzymic antioxidants (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase) and non-enzymic antioxidants (reduced glutathione, vitamin C and vitamin E) were observed in DMH-induced rats. Supplementation of EA attenuated all these alterations to near normal levels, which indicates the anti-carcinogenic efficacy of EA. This effect was further confirmed by histopathological studies. The results obtained in the present study suggest EA as an effective chemopreventive agent on colon carcinogenesis induced by DMH.

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Section: Resultsmentioning

confidence: 99%

Chemomodulation of the antioxidative enzymes and peroxidative damage in the colon of 1,2‐dimethyl hydrazine‐induced rats by ellagicacid

Umesalma

Sudhandiran

2010

Phytotherapy Research

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“…Compelling evidence indicates that HO-1 is implicated in the protection of lung tissue against oxidant exposure [20] and induction of HO-1 confers cytoprotection against oxidative stress both in vitro and in vivo [2]. Although few studies have found increased antioxidant activities both in tumour cells and in bronchoalveolar lavage of individuals with lung cancer [21,22], other studies have shown reduced antioxidant activities in lung tumours [23,24]. Particularly, Chung-Man Ho et al [9] described a decreased expression of the anti-oxidant enzyme catalase in lung tumour tissue as compared to tumour-free lung.…”

Section: Discussionmentioning

confidence: 96%

Decreased heme-oxygenase (HO)-1 in the macrophages of non-small cell lung cancer

Boschetto

Zeni

et al. 2008

Lung Cancer

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“…Out of all mesothelioma cell lines established from the tumours of our untreated patients, M14K cells, which were used here, have the lowest MnSOD activity. Previously, MnSOD has been reported to be low in most tumours and tumour cells (Sinha et al, 1990;Wong, 1995;Guner et al, 1996;Stammler et al, 1996), and it appears to have antiproliferative effects (Church et al, 1993;Li et al, 1995). However, the role of MnSOD in tumours is more complicated than originally assumed.…”

Section: Discussionmentioning

confidence: 99%

Endogenous antioxidant enzymes and glutathione S-transferase in protection of mesothelioma cells against hydrogen peroxide and epirubicin toxicity

Kinnula

Linnainmaa²,

Ko³

et al. 1998

Br J Cancer

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Summary We have previously shown that cultured malignant mesothelioma cells contain elevated manganese superoxide dismutase (MnSOD) mRNA levels and activities compared with non-malignant mesothelial cells. As many cytotoxic drugs generate both superoxide and hydrogen peroxide, we assessed the relative significance of catalase and the glutathione redox cycle, as well as glutathione S-transferase (GST), in protecting these cells against hydrogen peroxide and epirubicin toxicity. Mesothelioma cell lines containing high (M38K cells) and low (M14K cells) MnSOD, and non-malignant MeT-5A mesothelial cells were selected for the study. M38K cells were the most resistant of these three cell types to hydrogen peroxide (0.1-0.5 mm, 4 h) and epirubicin (0.1-0.5,g ml-', 48 h) as judged by lactate dehydrogenase (LDH) release and by high-energy nucleotide (ATP, ADP, AMP) depletion. Total glutathione was higher in M38K cells (63.8 ± 20.3 nnmol mg-1 protein) than in M14K (25.2 ± 8.2 nmol mg-') or MeT-5A cells (23.5 ± 4.5 nmol mg-'). Furthermore, GST specific activity was higher in M38K cells (111.3 ± 15.8 U mg-') than in M14K cells (77.4 ± 6.6 U mg-') or in MeT-5A cells (68.8 ± 7.6 U mg-'). Western blotting indicated the presence of GST-i in all these cells, the reactivity again being highest in M38K cells. Depletion of glutathione by buthionine sulphoximine and inhibition of catalase by aminotriazole enhanced hydrogen peroxide toxicity in all cell types, while only the depletion of glutathione increased epirubicin toxicity. We conclude that simultaneous induction of multiple antioxidant enzymes can occur in human mesothelioma cells. In addition to the high MnSOD activity, hydrogen peroxide scavenging antioxidant enzymes, glutathione and GST can partly explain the high hydrogen peroxide and epirubicin resistance of these cells in vitro.

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Evaluation of some antioxidant enzymes in lung carcinoma tissue

Cited by 50 publications

References 20 publications

Chemomodulation of the antioxidative enzymes and peroxidative damage in the colon of 1,2‐dimethyl hydrazine‐induced rats by ellagicacid

Chemomodulation of the antioxidative enzymes and peroxidative damage in the colon of 1,2‐dimethyl hydrazine‐induced rats by ellagicacid

Decreased heme-oxygenase (HO)-1 in the macrophages of non-small cell lung cancer

Endogenous antioxidant enzymes and glutathione S-transferase in protection of mesothelioma cells against hydrogen peroxide and epirubicin toxicity

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