Evaluation of Solanum species for resistance to Tomato leaf curl New Delhi virus using chip grafting assay

Akhtar, Khalid Pervaiz; Akram, Afzal; Ullah, Najeeb; Saleem, Muhammad; Saeed, Muhammad

doi:10.1016/j.scienta.2019.108646

Cited by 8 publications

(4 citation statements)

References 26 publications

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“…Tomato cultivars carrying Ty1/Ty3 genes are described as displaying resistant behavior after ToLCNDV inoculation ( Fortes et al, 2016 ; Hussain et al, 2019 ). However, the genetic background effect might modulate the effectiveness of Ty1/Ty3 for resistance to ToLCNDV in tomato ( Rai et al, 2013 ; Akhtar et al, 2019 ). In this work, we identify two SNPs generating missense variations in MELO3C015406.2, but QTLs linked to ToLCNDV resistance have not been mapped covering this region in previous works.…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq Transcriptome Analysis Provides Candidate Genes for Resistance to Tomato Leaf Curl New Delhi Virus in Melon

et al. 2022

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Tomato leaf curl New Delhi virus (ToLCNDV) emerged in the Mediterranean Basin in 2012 as the first DNA bipartite begomovirus (Geminiviridae family), causing severe yield and economic losses in cucurbit crops. A major resistance locus was identified in the wild melon accession WM-7 (Cucumis melo kachri group), but the mechanisms involved in the resistant response remained unknown. In this work, we used RNA-sequencing to identify disease-associated genes that are differentially expressed in the course of ToLCNDV infection and could contribute to resistance. Transcriptomes of the resistant WM-7 genotype and the susceptible cultivar Piñonet Piel de Sapo (PS) (C. melo ibericus group) in ToLCNDV and mock inoculated plants were compared at four time points during infection (0, 3, 6, and 12 days post inoculation). Different gene expression patterns were observed over time in the resistant and susceptible genotypes in comparison to their respective controls. Differentially expressed genes (DEGs) in ToLCNDV-infected plants were classified using gene ontology (GO) terms, and genes of the categories transcription, DNA replication, and helicase activity were downregulated in WM-7 but upregulated in PS, suggesting that reduced activity of these functions reduces ToLCNDV replication and intercellular spread and thereby contributes to resistance. DEGs involved in the jasmonic acid signaling pathway, photosynthesis, RNA silencing, transmembrane, and sugar transporters entail adverse consequences for systemic infection in the resistant genotype, and lead to susceptibility in PS. The expression levels of selected candidate genes were validated by qRT-PCR to corroborate their differential expression upon ToLCNDV infection in resistant and susceptible melon. Furthermore, single nucleotide polymorphism (SNPs) with an effect on structural functionality of DEGs linked to the main QTLs for ToLCNDV resistance have been identified. The obtained results pinpoint cellular functions and candidate genes that are differentially expressed in a resistant and susceptible melon line in response to ToLCNDV, an information of great relevance for breeding ToLCNDV-resistant melon cultivars.

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Section: Discussionmentioning

confidence: 99%

RNA-Seq Transcriptome Analysis Provides Candidate Genes for Resistance to Tomato Leaf Curl New Delhi Virus in Melon

et al. 2022

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“…However, varieties offering at least some level of protection seem available for some species. In tomato, some lines with the Ty-2 and Ty-3 genes showed lower disease incidence to ToLCNDV (Prasanna et al, 2015;Akhtar et al, 2019). Some C. maxima varieties show delay in symptoms expression and resistance/tolerance (mild or no symptoms and low virus load) has also been identified in C. moschata accessions (S aez et al, 2016).…”

Section: Use Of Resistant and Tolerant Plant Species/ Varietiesmentioning

confidence: 99%

Pest categorisation of tomato leaf curl New Delhi virus

Bragard¹,

Dehnen‐Schmutz²,

Serio³

et al. 2020

EFS2

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Following a request from the European Commission, the Panel on Plant Health performed a pest categorisation on tomato leaf curl New Delhi virus (ToLCNDV). ToLCNDV is a well‐defined bipartite Begomovirus species, sometimes associated with satellite molecules. It is transmitted by Bemisia tabaci to a wide range of hosts. ToLCNDV is reported from Estonia, Greece, Italy, Portugal and Spain, with limited distribution. The prevalent strain (ToLCNDV‐ES) in these countries is particularly adapted to cucurbits and is different from isolates reported outside the EU, which are better adapted to solanaceous crops and could therefore pose additional risk for EU agriculture. The virus is regulated under Commission Implementing Regulation (EU) 2019/2072. The main pathway of entry identified is plants for planting of susceptible hosts, even if entry could also occur via commodities carrying viruliferous B. tabaci and possibly by seeds. While establishment and local spread rely on B. tabaci, the virus can also be dispersed over long distances by movement of infected plants for planting. Establishment and spread are limited to regions with ecoclimatic conditions suitable for the establishment of vector populations (southern regions of Europe) or can occur as outbreaks wherever crops are grown under protected cultivation. The main uncertainties associated with this pest categorisation are the distribution and prevalence of ToLCNDV in the EU, the magnitude of the virus impact particularly on hosts different from Cucurbitaceae, and seed transmission. ToLCNDV meets all the criteria evaluated by EFSA to qualify as potential Union Quarantine Pest (QP); conversely, ToLCNDV does not meet the criterion of being widespread in the EU to qualify as a Regulated Non‐Quarantine Pest (RNQP). Should new data show that ToLCNDV is widespread in the EU, the possibility would exist for non‐EU isolates to qualify as QP, while ToLCNDV EU isolates (ToLCNDV‐ES) could qualify as RNQP.

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“…Grafting might improve growth, fruit yield (Zhang et al 2021), fruit quality traits (Zhou et al 2022), resistances against soil-borne diseases (Manickam et al 2021;Shalaby et al 2022), foliar diseases like tomato mosaic virus (Akhtar et al 2019;Span o et al 2020), tolerance to abiotic stresses such as salinity (Sanwal et al 2022;Zeist et al 2023), drought (El-Mogy et al 2022Kazemi et al 2021), and waterlogging, or hot and wet seasons (Evy and Rima 2020;Ray et al 2023), and high temperatures (Lee et al 2023). Rootstocks are usually designated to improve the tolerance of tomato scions to abiotic stress such as heat stress (Lee et al 2023), which is achieved through translocation between rootstocks and scions of hormones, proteins, and nucleic acids, which can function as signaling molecules that alter the gene expression of scion genotypes (Balfag on et al 2021;Shalaby et al 2022).…”

mentioning

confidence: 99%

Interspecific Hybrid Rootstocks Improve Productivity of Tomato Grown under High-temperature Stress

Hashem,

Bayoumi,

El-Zawily

et al. 2024

horts

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Grafting can be a useful technology to improve productivity of vegetable crops, including tomato, particularly under the serious challenges of climate change for agricultural systems. This study aimed to evaluate the impact of some local tomato interspecific hybrid rootstocks along with Maxifort on the vegetative growth, productivity, and fruit quality of tomato under field production conditions. Heat-tolerant tomato hybrid 023 F1 was used as a scion over the two late summer seasons of 2021 and 2022. Grafting 023 F1 onto Maxifort or KFS-16 rootstocks resulted in the maximum plant growth. Similarly, Maxifort and KFS-16 rootstocks significantly increased the fruit setting percentage from 22.2% to 23.5% and 17.8% to 24.6%, total fruit yield from 33.5% to 53.7% and 29.6% to 51.6%, and marketable yields from 34.1% to 56.0% and 27.3% to 56.7%, respectively, during both seasons compared with nongrafted plants. These two rootstocks enhanced nutrient (nitrogen, phosphorus, potassium) uptake compared with nongrafted planted. However, grafting with the interspecific hybrid rootstocks (KFS-8 and KWS-9) significantly decreased the content of catalase, peroxidase, and proline, which might be associated with lower plant vigor and yield in these rootstocks. All rootstocks had an impact on fruit chemical composition; however, generally, Maxifort and KFS-16 had greater contents of vitamin C, β-carotene, and total antioxidants than nongrafted plants. KFS-16 had also greater lycopene content than nongrafted plants. These results demonstrate the potential use of Maxifort and local rootstock KFS-16 to boost the growth and yield of tomato plants under high-temperature stress in the late summer season.

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Evaluation of Solanum species for resistance to Tomato leaf curl New Delhi virus using chip grafting assay

Cited by 8 publications

References 26 publications

RNA-Seq Transcriptome Analysis Provides Candidate Genes for Resistance to Tomato Leaf Curl New Delhi Virus in Melon

RNA-Seq Transcriptome Analysis Provides Candidate Genes for Resistance to Tomato Leaf Curl New Delhi Virus in Melon

Pest categorisation of tomato leaf curl New Delhi virus

Interspecific Hybrid Rootstocks Improve Productivity of Tomato Grown under High-temperature Stress

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