Evaluation of soil gram-negative bacteria yielding polyhydroxyalkanoic acids from carbohydrates and propionic acid

Gomez, José Gregório Cabrera; Rodrigues, M. F. A.; Alli, R. C. P.; Torres, Bayardo Baptista; Netto, C L Bueno; Oliveira, M. S.; Silva, L. F. da

doi:10.1007/s002530050763

Cited by 107 publications

(100 citation statements)

References 23 publications

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“…The granular bodies within the intracellular bacteria are likely to be cytoplasmic accumulations of polyhydroxyalkanoates which are known to occur in Burkholderia spp. (17,29). Lysates of inoculated spores consistently yielded PCR products identical to those of the inoculated bacteria.…”

Section: Discussionmentioning

confidence: 82%

Invasion of Spores of the Arbuscular Mycorrhizal Fungus Gigaspora decipiens by Burkholderia spp

Levy

Chang

Abbott³

et al. 2003

Appl Environ Microbiol

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Burkholderia species are bacterial soil inhabitants that are capable of interacting with a variety of eukaryotes, in some cases occupying intracellular habitats. Pathogenic and nonpathogenic Burkholderia spp., including B. vietnamiensis, B. cepacia, and B. pseudomallei, were grown on germinating spores of the arbuscular mycorrhizal fungus Gigaspora decipiens. Spore lysis assays revealed that all Burkholderia spp. tested were able to colonize the interior of G. decipiens spores. Amplification of specific DNA sequences and transmission electron microscopy confirmed the intracellular presence of B. vietnamiensis. Twelve percent of all spores were invaded by B. vietnamiensis, with an average of 1.5 ؋ 10 6 CFU recovered from individual infected spores. Of those spores inoculated with B. pseudomallei, 7% were invaded, with an average of 5.5 ؋ 10 5 CFU recovered from individual infected spores. Scanning electron and fluorescence microscopy provided insights into the morphology of surfaces of spores and hyphae of G. decipiens and the attachment of bacteria. Burkholderia spp. colonized both hyphae and spores, attaching to surfaces in either an end-on or side-on fashion. Adherence of Burkholderia spp. to eukaryotic surfaces also involved the formation of numerous fibrillar structures.

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Section: Discussionmentioning

confidence: 82%

Invasion of Spores of the Arbuscular Mycorrhizal Fungus Gigaspora decipiens by Burkholderia spp

Levy

Chang

Abbott³

et al. 2003

Appl Environ Microbiol

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“…The Tukey test was used to determine differences between the variables' temperature and substrate, with a 95 % confidence interval. ) (Gomez et al, 1996). However, the highest PHA productivity (0.054 g L −1 h −1 ) was seen with glucose at 35 °C.…”

Section: Discussionmentioning

confidence: 97%

Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures

Nascimento

Silva

Gomez

et al. 2016

Sci. agric. (Piracicaba, Braz.)

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Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no differences in growth rates (μ max ) in sucrose and glucose. The growth conditions studied were not favorable to glycerol consumption due to limitations in the energy supply from glycerol.

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“…The strain which is used for PHA accumulation is an important factor in the production of poly(3HB-co-3HV). Many bacteria exhibit only a low 3HV yield with propionic acid (Y 3HV/Prop ); in most cases this yield is approximately 0.1 g ⅐ g Ϫ1 , whereas the theoretical value for the yield is 1.35 g ⅐ g Ϫ1 (17). The low yield may result from the predominance of oxidative pathways in propionic acid metabolism and the ability of the organisms to produce 3-hydroxybutyrate from this substrate.…”

mentioning

confidence: 93%

“…Wild-type strain B. sacchari IPT101 T (Suc ϩ Prp ϩ ) (7,17) and mutant IPT189 (Suc ϩ Prp Ϫ ) obtained from IPT101 T (41) were studied. E. coli S17-1 (42) and cosmids pVK100 (27) and pHC79 (20) were used in cloning experiments.…”

Section: Methodsmentioning

confidence: 99%

Identification of the 2-Methylcitrate Pathway Involved in the Catabolism of Propionate in the Polyhydroxyalkanoate-Producing Strain Burkholderia sacchari IPT101 ^T and Analysis of a Mutant Accumulating a Copolyester with Higher 3-Hydroxyvalerate Content

Brämer

Silva

Gomez

et al. 2002

Appl Environ Microbiol

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Burkholderia sacchari IPT101T induced the formation of 2-methylcitrate synthase and 2-methylisocitrate lyase when it was cultivated in the presence of propionic acid. The prp locus of B. sacchari IPT101 T is required for utilization of propionic acid as a sole carbon source and is relevant for incorporation of 3-hydroxyvalerate (3HV) into copolyesters, and it was cloned and sequenced. Five genes (prpR, prpB, prpC, acnM, and ORF5) exhibited identity to genes located in the prp loci of other gram-negative bacteria. prpC encodes a 2-methylcitrate synthase with a calculated molecular mass of 42,691 Da. prpB encodes a 2-methylisocitrate lyase. The levels of PrpC and PrpB activity were much lower in propionate-negative mutant IPT189 obtained from IPT101 T and were heterologously expressed in Escherichia coli. The acnM gene (ORF4) and ORF5, which are required for conversion of 2-methylcitric acid to 2-methylisocitric acid in Ralstonia eutropha HF39, are also located in the prp locus. The translational product of ORF1 (prpR) had a calculated molecular mass of 70,598 Da and is a putative regulator of the prp cluster. Three additional open reading frames (ORF6, ORF7, and ORF8) whose functions are not known were located adjacent to ORF5 in the prp locus of B. sacchari, and these open reading frames have not been found in any other prp operon yet. In summary, the organization of the prp genes of B. sacchari is similar but not identical to the organization of these genes in other bacteria investigated recently. In addition, this study provided a rationale for the previously shown increased molar contents of 3HV in copolyesters accumulated by a B. sacchari mutant since it was revealed in this study that the mutant is defective in prpC.Polyhydroxyalkanoates (PHAs) are natural thermoplastics that occur in a wide variety of bacteria which synthesize these polyesters as carbon and energy storage compounds from renewable carbon sources. The thermoplastic properties of the copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV); Biopol] and its biodegradability are influenced mainly by the content of 3-hydroxyvalerate (3HV), which improves the properties of the molecule and also extends the range of its applications (21). Therefore, since PHAs can be obtained from renewable resources, commercial interest in these polyesters has increased a great deal during the last decade. Wild-type strains of different bacteria and mutants which are able to produce 3HV from unrelated carbon sources have been studied in the last few years (44,45,53).The biotechnological processes now used for fermentative production of poly(3HB-co-3HV) by bacteria depend on a supply of propionic acid as a specific 3HV precursor substrate in addition to a carbohydrate or another main carbon source. This requirement allows workers to control the composition of the polyester by changing the ratio of propionic acid to glucose (carbohydrate) in the medium (12). The strain which is used for PHA accumulation is an important factor in the production of ...

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Evaluation of soil gram-negative bacteria yielding polyhydroxyalkanoic acids from carbohydrates and propionic acid

Cited by 107 publications

References 23 publications

Invasion of Spores of the Arbuscular Mycorrhizal Fungus Gigaspora decipiens by Burkholderia spp

Invasion of Spores of the Arbuscular Mycorrhizal Fungus Gigaspora decipiens by Burkholderia spp

Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures

Identification of the 2-Methylcitrate Pathway Involved in the Catabolism of Propionate in the Polyhydroxyalkanoate-Producing Strain Burkholderia sacchari IPT101 ^T and Analysis of a Mutant Accumulating a Copolyester with Higher 3-Hydroxyvalerate Content

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Evaluation of soil gram-negative bacteria yielding polyhydroxyalkanoic acids from carbohydrates and propionic acid

Cited by 107 publications

References 23 publications

Invasion of Spores of the Arbuscular Mycorrhizal Fungus Gigaspora decipiens by Burkholderia spp

Invasion of Spores of the Arbuscular Mycorrhizal Fungus Gigaspora decipiens by Burkholderia spp

Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures

Identification of the 2-Methylcitrate Pathway Involved in the Catabolism of Propionate in the Polyhydroxyalkanoate-Producing Strain Burkholderia sacchari IPT101 T and Analysis of a Mutant Accumulating a Copolyester with Higher 3-Hydroxyvalerate Content

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Identification of the 2-Methylcitrate Pathway Involved in the Catabolism of Propionate in the Polyhydroxyalkanoate-Producing Strain Burkholderia sacchari IPT101 ^T and Analysis of a Mutant Accumulating a Copolyester with Higher 3-Hydroxyvalerate Content