Evaluation of sodium valproate loaded nanoparticles in acute and chronic pentylenetetrazole induced seizure models

Meenu, Meenakshi; Reeta, K.H.; Dinda, Amit Kumar; Kottarath, Sarat Kumar; Gupta, Yogendra Kumar

doi:10.1016/j.eplepsyres.2019.106219

Cited by 14 publications

(8 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PTZ is a GABA A R antagonist that induces generalised tonic-clonic seizures after intraperitoneal injection. Seizures were induced using PTZ as previously described 37 , 59 – 61 . In the present study, adult mice were intraperitoneally injected with a single dose of PTZ (30 mg/kg) (#P6500; Sigma-Aldrich Inc.) dissolved in 0.9% saline, and recorded during the first 30 min after administration.…”

Section: Methodsmentioning

confidence: 99%

Neocortex- and hippocampus-specific deletion of Gabrg2 causes temperature-dependent seizures in mice

Guo

et al. 2021

Cell Death Dis

View full text Add to dashboard Cite

Mutations in the GABRG2 gene encoding the γ-aminobutyric acid (GABA) A receptor gamma 2 subunit are associated with genetic epilepsy with febrile seizures plus, febrile seizures plus, febrile seizures, and other symptoms of epilepsy. However, the mechanisms underlying Gabrg2-mediated febrile seizures are poorly understood. Here, we used the Cre/loxP system to generate conditional knockout (CKO) mice with deficient Gabrg2 in the hippocampus and neocortex. Heterozygous CKO mice (Gabrg2fl/wtCre+) exhibited temperature-dependent myoclonic jerks, generalised tonic-clonic seizures, increased anxiety-like symptoms, and a predisposition to induce seizures. Cortical electroencephalography showed the hyperexcitability in response to temperature elevation in Gabrg2fl/wtCre+ mice, but not in wild-type mice. Gabrg2fl/wtCre+ mice exhibited spontaneous seizures and susceptibility to temperature-induced seizures. Loss of neurons were observed in cortical layers V–VI and hippocampus of Gabrg2fl/wtCre+ mice. Furthermore, the latency of temperature- or pentylenetetrazol-induced seizures were significantly decreased in Gabrg2fl/wtCre+ mice compared with wild-type mice. In summary, Gabrg2fl/wtCre+ mice with Gabrg2 deletion in the neocortex and hippocampus reproduce many features of febrile seizures and therefore provide a novel model to further understand this syndrome at the cellular and molecular level.

show abstract

Section: Methodsmentioning

confidence: 99%

Neocortex- and hippocampus-specific deletion of Gabrg2 causes temperature-dependent seizures in mice

Guo

et al. 2021

Cell Death Dis

View full text Add to dashboard Cite

show abstract

“…Epilepsy is a chronic neurological disorder manifesting as spontaneous, recurrent and unpredictable seizures resulting from transient abnormal electrical activity in the brain 15 , 60 . The main causes of epilepsy include infection progression in the brain, reduced oxygen supply, head trauma, arteriovenous malformations, cerebrovascular diseases and perinatal injuries 50 .…”

Section: Normal Bbb and Diseased Bbbmentioning

confidence: 99%

Evolution of blood–brain barrier in brain diseases and related systemic nanoscale brain-targeting drug delivery strategies

Han

Jiang

2021

Acta Pharmaceutica Sinica B

173

105

View full text Add to dashboard Cite

Blood–brain barrier (BBB) strictly controls matter exchange between blood and brain, and severely limits brain penetration of systemically administered drugs, resulting in ineffective drug therapy of brain diseases. However, during the onset and progression of brain diseases, BBB alterations evolve inevitably. In this review, we focus on nanoscale brain-targeting drug delivery strategies designed based on BBB evolutions and related applications in various brain diseases including Alzheimer's disease, Parkinson's disease, epilepsy, stroke, traumatic brain injury and brain tumor. The advances on optimization of small molecules for BBB crossing and non-systemic administration routes ( e . g ., intranasal treatment) for BBB bypassing are not included in this review.

show abstract

“…Gabapentin was the antiepileptic agent of choice in the investigation conducted by Wilson et al [22], and lacosamide was employed in a study conducted by Temizyürek et al [23]. Sodium valproate was administered in the research undertaken by Meenu et al [24], and clonazepam was the selected antiepileptic medication in the study conducted by Leyva-Gómez et al [25]. Additionally, oxcarbazepine was employed in the investigation by Musumeci et al [26].…”

Section: Characteristics Of Included Studiesmentioning

confidence: 99%

Unveiling the effects of nanoparticles-based antiepileptic drugs: Systematic review of in vivo studies

Husnul Khotimah,

Fathina Zahrani Rahmaniar,

Fatimah Az Zahra

et al. 2024

GSC Biol. Pharm. Sci.

View full text Add to dashboard Cite

Background: Resistance and side effects of antiepileptic drugs (AEDs) pose a challenge in epilepsy therapy due to the limited drug bioavailability in penetrating the Blood-Brain Barrier (BBB). Nanoparticles can be one solution by encapsulating AEDs to enhance drug distribution to target cells. This study systematically assesses 1) the characteristics of nanoparticles, and 2) the potential of nanoparticle AEDs in managing seizures in experimental animal models. Methods: This systematic literature review is limited to studies published between 2013 and July 2023 in the PubMed, ScienceDirect, ProQuest, MEDLINE, and Scopus databases. Inclusion criteria encompass studies involving animal models of epilepsy, that exploring nanoparticle-based of AEDs. These studies compare the characteristics of nanoparticles and their antiepileptic efficacy with non-nanoparticle groups. Review articles, publications in non-English languages, and ongoing studies without published results are excluded. Result and Discussion: Fourteen studies met the inclusion criteria for this research. All studies utilized nanoparticles (n = 14). Lipid nanoparticles have a more compact size than any other nanoparticle, while the combination preparation method has an optimal nanoparticle formation in both lipid and polymeric nanoparticles. In animal model results indicated that nanoparticle-based drugs were beneficial in reducing seizure scores, improving seizure onset latency, and providing neuroprotective effects. Conclusion: The characteristics of nanoparticle drug delivery varied, influenced by formulation factors and preparation methods. Nanoparticle-based AEDs exhibit higher efficacy compared to conventional AEDs. All studies included present an opportunity for the development of epilepsy therapies, although future studies are needed to confirm these findings.

show abstract

Evaluation of sodium valproate loaded nanoparticles in acute and chronic pentylenetetrazole induced seizure models

Cited by 14 publications

References 68 publications

Neocortex- and hippocampus-specific deletion of Gabrg2 causes temperature-dependent seizures in mice

Neocortex- and hippocampus-specific deletion of Gabrg2 causes temperature-dependent seizures in mice

Evolution of blood–brain barrier in brain diseases and related systemic nanoscale brain-targeting drug delivery strategies

Unveiling the effects of nanoparticles-based antiepileptic drugs: Systematic review of in vivo studies

Contact Info

Product

Resources

About