Evaluation of Sludge Palm Oil as Feedstock and Development of Efficient Method for its Utilization to Produce Polyhydroxyalkanoate

Thinagaran, L.; Sudesh, Kumar

doi:10.1007/s12649-017-0078-8

Cited by 42 publications

(24 citation statements)

References 39 publications

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“…A recent study by Thinagaran and Sudesh has revealed that emulsified SPO, when supplemented at 10 g/L to the engineered C. necator strain Re2058/pCB113, produced as high as 9.7 g/L of dried cells mass in which 74 wt% contained P(3HB-co-21 mol% 3HHx). Further improvements in the yield were achieved by employing a fed-batch fermentation strategy, which resulted in biomass productivity of 1.9 g/L/h and PHA productivity of 1.1 g/L/h (Thinagaran and Sudesh, 2019). Table 1 summarizes all the bacterial strains and the respective waste plant oil feedstocks that were used for PHA production.…”

Section: Biosynthesis Of Phas Using Waste Plant Oilsmentioning

confidence: 99%

“…One of the major factors that determine efficient utilization of these waste oils is its assimilability by the microbes for PHA biosynthesis. Although feedstocks such as SPO are rich in FFAs, it was found to solidify instantaneously when added to the culture medium (Thinagaran and Sudesh, 2019) causing the oil to be not available homogenously in the bacterial culture. This has resulted in the conversion yield of SPO to biomass to be lower than conversion yield of CPKO to biomass (Thinagaran and Sudesh, 2019).…”

Section: Factors Affecting the Efficient Utilization Of Waste Plant Amentioning

confidence: 99%

“…Although feedstocks such as SPO are rich in FFAs, it was found to solidify instantaneously when added to the culture medium (Thinagaran and Sudesh, 2019) causing the oil to be not available homogenously in the bacterial culture. This has resulted in the conversion yield of SPO to biomass to be lower than conversion yield of CPKO to biomass (Thinagaran and Sudesh, 2019). SPO was made readily available for the bacterial strains when emulsified using surfactants.…”

Section: Factors Affecting the Efficient Utilization Of Waste Plant Amentioning

confidence: 99%

“…Based on the observations in shake flask experiments, the biosynthesis is usually scaled up in a bioreactor system and this is where many parameters have to be carefully studied and manipulated to generate a maximum conversion yield of carbon feedstock to biomass. Based on the reported studies using bioreactors for PHA production, it was found that fed-batch fermentation strategy and/or pulse-feeding of carbon feedstocks are the preferred methods for efficient PHA production using waste plant or animal oils Ciesielski, 2013, 2014;Riedel et al, 2015;Zainab-L et al, 2018;Thinagaran and Sudesh, 2019). Employing a suitable fermentation strategy also goes hand in hand with a proper control of some of the key bioreactor conditions such as dissolved oxygen (DO), impeller design, agitation speed of impeller, aeration rate, pH, and temperature control for an efficient utilization of waste oil feedstocks for PHA production.…”

Section: Factors Affecting the Efficient Utilization Of Waste Plant Amentioning

confidence: 99%

“…Among the waste streams identified, there is convincing scientific evidence in support of the higher carbon content in oilbased feedstocks (Akiyama et al, 2003). So far, PHA production using waste plant oils (e.g., palm kernel oil, crude palm oil, palm oil, jatropha oil, sludge palm oil, soybean oil) as inexpensive carbon sources has been taken into consideration owing to its widespread availability and renewability (Lee et al, 2008;Sudesh et al, 2011;Rodrigues et al, 2019;Thinagaran and Sudesh, 2019). Different agriculture sectors generate different types of waste.…”

Section: Introductionmentioning

confidence: 99%

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Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

Surendran

Lakshmanan

Chee

et al. 2020

Front. Bioeng. Biotechnol.

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111

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Polyhydroxyalkanoates (PHAs) are a potential replacement for some petrochemical-based plastics. PHAs are polyesters synthesized and stored by various bacteria and archaea in their cytoplasm as water-insoluble inclusions. PHAs are usually produced when the microbes are cultured with nutrient-limiting concentrations of nitrogen, phosphorus, sulfur, or oxygen and excess carbon sources. Such fermentation conditions have been optimized by industry to reduce the cost of PHAs produced commercially. Industrially, these biodegradable polyesters are derived from microbial fermentation processes utilizing various carbon sources. One of the major constraints in scaling-up PHA production is the cost of the carbon source metabolized by the microorganisms. Hence, cheap and renewable carbon substrates are currently being investigated around the globe. Plant and animal oils have been demonstrated to be excellent carbon sources for high yield production of PHAs. Waste streams from oil mills or the used oils, which are even cheaper, are also used. This approach not only reduces the production cost for PHAs, but also makes a significant contribution toward the reduction of environmental pollution caused by the used oil. Advancements in the genetic and metabolic engineering of bacterial strains have enabled a more efficient utilization of various carbon sources, in achieving high PHA yields with specified monomer compositions. This review discusses recent developments in the biosynthesis and classification of various forms of PHAs produced using crude and waste oils from the oil palm and fish industries. The biodegradability of the PHAs produced from these oils will also be discussed.

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Section: Biosynthesis Of Phas Using Waste Plant Oilsmentioning

confidence: 99%

Section: Factors Affecting the Efficient Utilization Of Waste Plant Amentioning

confidence: 99%