Evaluation of single nucleotide polymorphisms in microRNAs (hsa-miR-196a2 rs11614913 C/T) from Brazilian women with breast cancer

Abstract

BackgroundEmerging evidence has shown that miRNAs are involved in human carcinogenesis as tumor suppressors or oncogenes. Single nucleotide polymorphisms (SNPs) located in pre-miRNAs may affect the processing and therefore, influence the expression of mature miRNAs. Previous studies generated conflicting results when reporting association between the hsa-miR-196a2 rs11614913 common polymorphism and breast cancer.MethodsThis study evaluated the hsa-miR-196a2 rs11614913 SNP in 388 breast cancer cases and 388 controls in Brazilian women. Polymorphism was determined by real-time PCR; control and experimental groups were compared through statistical analysis using the X2 or Fisher’s exact tests.ResultsThe analysis of the SNPs frequencies showed a significant difference between the groups (BC and CT) in regards to genotype distribution (χ2: p = 0.024); the homozygous variant (CC) was more frequent in the CT than in the BC group (p = 0.009). The presence of the hsa-miR-196a2 rs11614913 C/T polymorphism was not associated with histological grades (p = 0.522), axillary lymph node positive status (p = 0.805), or clinical stage (p = 0.670) among the breast cancer patients.ConclusionsThe results of this study indicated that the CC polymorphic genotype is associated with a decreased risk of BC and the presence of the T allele was significantly associated with an increased risk of BC.

The Associations of Single Nucleotide Polymorphisms in miR-146a, miR-196a and miR-499 with Breast Cancer Susceptibility

“…The article published by Alshatwi contained discrepancies between the data shown in the tables and the data described in the results section [14]; therefore, after consultation with the author, these data were excluded. In Catucci's [19] and Linhares's [28] studies, the genotype frequencies were presented according to the subjects' country or race, as in previous reports [29], [30]; thus in the present analysis each group was considered as an independent study. Moreover, in some included articles, if two or more miRNA SNPs were investigated in an article, each miRNA SNP was considered as an independent study.…”

Associations Between Three Common MicroRNA Polymorphisms and Hepatocellular Carcinoma Risk in Chinese

“…Previous studies indicated that miR-196a2C>T polymorphisms have been widely investigated in various cancers, such as non-small cell lung cancer, breast cancer, pancreatic cancer and esophageal cancer as well as gastric cancer (Zhan et al, 2012;Linhares et al, 2012;Pavlakis et al, 2013;Wei et al, 2013;Yuan et al, 2013). Three studies reported that miR-196a2C>T polymorphism are associated with development of HCC (Li et al, 2010;Qi et al, 2010).…”

Association Analysis of Single Nucleotide Polymorphisms in miR-146a and miR-196a2 on the Prevalence of Cancer in Elderly Japanese: A Case-Control Study

“…The nucleotide change of T to C may affect the miR-196a2 expression (Saunders et al, 2007;Chu et al, 2011). Recent studies show that rs11614913 may influence lung cancer (Tian et al, 2009;Hong et al, 2011;Yuan et al, 2013), breast cancer (Hoffman et al, 2009;Linhares et al, 2012), head and neck cancer (Christensen et al, 2010), hepatocellular carcinoma (Xu et al, 2008;Qi et al, 2010;Hao et al, 2013), gastric cancer (Peng et al, 2010), prostate cancer (George et al, 2011) and others . On the contrary, there are also several studies for both rs2910164 and rs11614913 polymorphisms indicate conflicting results asserting either lack of association or decreased cancer risk (Xu et al, 2011;Hezova et al, 2012;He et al, 2012;Xu et al, 2013;Zhang et al, 2013).…”