Evaluation of single and comparative intradermal tuberculin tests for tuberculosis eradication in caprine flocks in Castilla y León (Spain)

Bezos, Javier; Marques, Sérgio Corrêa; Álvarez, Julio; Casal, Carmen; Romero, Beatriz; Grau, Ana; Mínguez, Olga; Domı́nguez, Lucas; Juan, Lucía de

doi:10.1016/j.rvsc.2013.10.007

Cited by 21 publications

(25 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using the SCIT test, a bovine reaction greater than avian reaction more than 4 mm was considered as positive result. A more stringent interpretation considering inconclusive reactors to SIT (3 mm) or SCIT test (>1 and ≤4 mm) as positive reactors recommended in infected settings was also applied as described elsewhere (Bezos et al, 2014b).…”

Section: Methodsmentioning

confidence: 99%

Goats challenged with different members of the Mycobacterium tuberculosis complex display different clinical pictures

Bezos

Casal²,

Díez‐Delgado

et al. 2015

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Goats challenged with different members of the Mycobacterium tuberculosis complex display different clinical pictures

Bezos

Casal²,

Díez‐Delgado

et al. 2015

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

“…Published estimates of SICTT sensitivity range from 42.7 to 83.7%, while those of SIT range from 44.6 to 93.8% (2,3,5). However, a recent study adopting bacteriology as the gold standard showed the sensitivity of the SIT by the severe interpretation to be as low as 43.9% and to decrease to 38.8% by the standard interpretation, while the sensitivity of the SICTT ranged between 21.3 and 7%, depending of the interpretation criterion used (6).…”

mentioning

confidence: 99%

Serological Analysis of Tuberculosis in Goats by Use of the Enferplex Caprine TB Multiplex Test

O’Brien¹,

Whelan²,

Clarke³

et al. 2017

Clin Vaccine Immunol

View full text Add to dashboard Cite

Tuberculosis in goats is usually diagnosed clinically, at postmortem, or by a positive skin test. However, none of these approaches detects all infected animals. Serology offers an additional tool to identify infected animals missed by current tests. We describe the use of the Enferplex Caprine TB serology test to aid the management of a large dairy goat herd undergoing a tuberculosis breakdown. Initial skin and serology testing showed that IgG antibodies were present in both serum and milk from 100% of skin test-positive animals and in serum and milk from 77.8 and 95.4% of skin test-negative animals, respectively. A good correlation was observed between serum and milk antibody levels. The herd had been vaccinated against Mycobacterium avium subsp. paratuberculosis, but no direct serological crossreactions were found. Subsequent skin testing revealed 13.7% positive animals, 64.9% of which were antibody positive, while 42.1% of skin test-negative animals were seropositive. Antibody responses remained high 1 month later (57.1% positive), and the herd was slaughtered. Postmortem analysis of 20 skin test-negative goats revealed visible lesions in 6 animals, all of which had antibodies to six Mycobacterium bovis antigens. The results provide indirect evidence that serology testing with serum or milk could be a useful tool in the diagnosis and management of tuberculosis in goats.KEYWORDS tuberculosis, antibodies, goats T uberculosis (TB) due to infection by Mycobacterium bovis is a major problem in cattle in the United Kingdom, causing huge financial losses, as well as being a significant zoonosis risk. The presence of significant levels of TB in wildlife vectors such as the badger has led to trial culling or vaccination of these animals in high-risk areas such as southwestern England and Wales, respectively. Spillover hosts such as sheep, goats, deer, and alpacas also present a risk of spreading TB (1). Currently, control programs involving cattle are focused on detecting cell-mediated immunity (CMI) through the use of tuberculin skin testing and gamma interferon (IFN-␥) tests. In goats, the single intradermal comparative tuberculin test (SICTT) and the single intradermal test (SIT) are used to detect infection (2-5). Published estimates of SICTT sensitivity range from 42.7 to 83.7%, while those of SIT range from 44.6 to 93.8% (2, 3, 5). However, a recent study adopting bacteriology as the gold standard showed the sensitivity of the SIT by the severe interpretation to be as low as 43.9% and to decrease to 38.8% by the standard interpretation, while the sensitivity of the SICTT ranged between 21.3 and 7%, depending of the interpretation criterion used (6).Recent studies also showed that when goats are coinfected with Mycobacterium avium subsp. paratuberculosis, the sensitivity and specificity of the TB skin tests appear to be adversely affected, with a substantial proportion of M. bovis-infected animals going undetected (2-4, 7-11). Since skin and serology tests measure different arms of

show abstract

“…The TST is the internationally recognized standard method to identify infection with M. tuberculosis and M. bovis. Intradermal tuberculin tests based on eliciting a delayed-type hypersensitivity response, which is mediated by a population of sensitized T-cells and takes some weeks to develop after infection [52]. The TST use the purified protein derivative (PPD) tuberculins that have been derived from M. bovis and M. tuberculosis for detection of latent TB in cattle and in human, respectively.…”

Section: Tuberculin Skin Test (Tst)mentioning

confidence: 99%

“…Despite of the several major limitations, TST has been, until recently, a useful procedure for detecting LTBI [57] and most widely used test for control of Bovine TB due to the low-cost, low logistical demands, well-documented use, and its ability to screen the entire cattle populations [52]. Due to the limitations of the sensitivity and specificity of the skin tests, newer ancillary in vitro diagnostic assays for latent tuberculosis has been developed, which measures the production of interferons in whole blood upon stimulation with PPD [52].…”

Section: Tuberculin Skin Test (Tst)mentioning

confidence: 99%

Paralleling of Diagnostic Endeavor for Control of Mycobacterial Infections and Tuberculosis

Lupindu¹,

Mbugi²,

Nzalawahe³

et al. 2018

Basic Biology and Applications of Actinobacteria

View full text Add to dashboard Cite

Mycobacterial infections and tuberculosis pose global public health threats. High tuberculosis morbidities and mortalities are due to the diagnosis problems among other causes. This chapter describes and compares diverse mycobacterial infections and tuberculosis diagnostic efforts and point-out the direction so as to inform areas of and motivate research toward early, rapid, and accurate diagnosis for effective TB control. We have grouped diagnostic approaches according to the type of sample taken for or organ targeted during diagnosis. The sputum-based methods include smear microscopy, culture, and rat sniffing. Interferon-γ (INF-γ) release assays, transcriptional blood signatures, and proteomic profiling use blood samples while colorimetric sensor array (CSA) and mass spectrometry use urine samples. Patho-physiological methods include tuberculin skin tests (TSTs) and radiography. Chromatography and acoustic wave detection can also be used to diagnose TB from breath. Comparative description of these methods is based on a time frame to diagnosis, accuracy, cost, and convenience. The trend shows that there is a move from time-consuming, slow and narrow-spectrum to quick and broad-spectrum TB diagnostic procedures. The sputum-based and patho-physiological approaches remain conformist while blood-based procedures lead research developments. Absence of single best approach calls for synergistic research combinations that form accurate, rapid, cheap, and convenient package at point-of-care centers.

show abstract

Evaluation of single and comparative intradermal tuberculin tests for tuberculosis eradication in caprine flocks in Castilla y León (Spain)

Cited by 21 publications

References 27 publications

Goats challenged with different members of the Mycobacterium tuberculosis complex display different clinical pictures

Goats challenged with different members of the Mycobacterium tuberculosis complex display different clinical pictures

Serological Analysis of Tuberculosis in Goats by Use of the Enferplex Caprine TB Multiplex Test

Paralleling of Diagnostic Endeavor for Control of Mycobacterial Infections and Tuberculosis

Contact Info

Product

Resources

About